CAJ | 학술논문

目的观察自行研制合成的骨形成蛋白2(BMP2)活性多肽与Ⅰ型胶原复合煅烧骨复合材料修复骨缺损的作用.方法实验分3组.A组:单纯煅烧骨组;B组:含Ⅰ型胶原的煅烧骨组;C组:BMP2活性多肽与Ⅰ型胶原复合煅烧骨组.3组材料植入前分别电镜扫描观察.取大白兔36只,随机分成3组,建立桡骨中段10 mm骨缺损模型,分别将材料植入兔桡骨缺损处,术后4、8、12周行X线评分,8、12周行组织形态学观察与评分,比较各组植入材料的成骨作用以及取材后抗折强度.结果 36只大白兔均进入结果分析,(1)复合材料电镜扫描结果:孔隙率高,复合结构紧密;(2)术后不同时间X线评分示C组与A组和B组差异有统计学意义(P＜0.05);(3)组织学观察,8周时评分为:A组4.67±0.52、B组5.33±0.52、C组6.83±0.41.12周评分为:A组评分为6.17±0.41、B组为7.33±0.52、C组为8.67±0.52;(4)各组植入材料抗折强度的测定:术后12周,C组材料抗折强度明显高于A组和B组.结论单纯煅烧骨及复合材料组均有修复骨缺损的能力,但以复合BMP2活性多肽的含I型胶原的煅烧骨较为理想.
목적 관찰자행연제합성적골형성단백2(BMP2)활성다태여Ⅰ형효원복합단소골복합재료수복골결손적작용.방법 실험분3조.A조:단순단소골조;B조:함Ⅰ형효원적단소골조;C조:BMP2활성다태여Ⅰ형효원복합단소골조.3조재료식입전분별전경소묘관찰.취대백토36지,수궤분성3조,건립뇨골중단10 mm골결손모형,분별장재료식입토뇨골결손처,술후4、8、12주행X선평분,8、12주행조직형태학관찰여평분,비교각조식입재료적성골작용이급취재후항절강도.결과 36지대백토균진입결과분석,(1)복합재료전경소묘결과:공극솔고,복합결구긴밀;(2)술후불동시간X선평분시C조여A조화B조차이유통계학의의(P＜0.05);(3)조직학관찰,8주시평분위:A조4.67±0.52、B조5.33±0.52、C조6.83±0.41.12주평분위:A조평분위6.17±0.41、B조위7.33±0.52、C조위8.67±0.52;(4)각조식입재료항절강도적측정:술후12주,C조재료항절강도명현고우A조화B조.결론 단순단소골급복합재료조균유수복골결손적능력,단이복합BMP2활성다태적함I형효원적단소골교위이상.
Objective To observe the repair of bone defect with sintered bovine cancellous bone complexed with synthetized BMP2-derived peptide and collagen Ⅰ.Methods Rabbits were divided into 3 groups.Sintered bovine bone was implanted in group A.Sintered bovine bone complexed with collagen 1 was,implanted in group B.Sintered bovine bone complexed with BMP2-derived peptide/eollagen 1 was implanted in group C.Before implantation,the materials were observed under the environmental scanning electron microscopy(ESEM).Thirty-six rabbits were gained to establish models of 10-mm middle radius bone defects and divided into 3 groups with 12 in each group.Postoperatively.the formed new bone was compared among the three implants at the 4th,8th,12th week by X ray score,and at the 8th,12th week by histological score.The bend intensity of implanted materials at the 12th week were tested.respectively.Resuits Thirty-six rabbits were all involved in the resuh analysis.(1)ESEM revealed that the porosity of compound materials was high with close compound.(2)At different postoperative time points,the X ray scores on the group C were significantly greater than in groups A and B(P＜0.05).(3)The histological scores at the 4th and 8th week on the group C were 6.83±0.41 abnd 8.67±0.52 respectively.significanfly higher than in both group A(4.67±0.52,6.17±0.41)and group B(5.33±0.52,7.33±0.52)respectively(P＜0.05).(4)The bend intensity of implanted materials in group C at the 12th week after operation was higher significantly than that in groups A and B.Conclusion Sintered bovine cancellous bone and its compound materials can repair the bone defect,but the sintered bovine bone complexed with BMP2-derived peptide/collagen I is the most ideal.