CAJ | 학술논문

目的研究慢性乙型肝炎患者细胞毒T细胞内穿孔素(Perforin)、干扰素(IFN-γ)和白细胞介素10(IL-10)表达与病毒消除的关系.方法对50例慢性乙型肝炎患者外周血进行短期培养后,利用流式细胞术测定CD8~+细胞内Perforin、IFN-γ和IL-10表达,并与30例正常对照进行比较;利用荧光定量PCR检测慢性乙型肝炎患者HBV DNA的载量,分析其与CD8~+细胞内Perforin、IFN-γ和IL-10表达的关系.结果慢性乙型肝炎组CD8~+T细胞Perforin、IFN-γ和IL-10的表达分别为(5.30 ±2.62)%、(4.05±2.25)%和(0.77 ±0.50)%,明显低于正常对照组(t分别为4.50、4.56、4.20,P均<0.01);26例HBeAg阳性的慢性乙型肝炎患者CD8~+T细胞Pefforin和IFN-γ的表达分别为(4.54 ±1.93)%和(3.32 ±1.59)%,明显低于24例HBeAg阴性的慢性乙型肝炎患者(t分别为2.22、2.54,P均<0.05);慢性乙型肝炎患者CD8~+T细胞内Perforin和IFN-γ表达率与乙肝病毒DNA载量成负相关(r分别为-0.539、-0.340,P<0.01、P<0.05).结论慢性乙型肝炎患者细胞毒T细胞内Perforin、IFN-γ和IL-10表达减少,可能与慢性乙型肝炎患者病毒长期存在,病程迁延不愈有关.
목적 연구만성을형간염환자세포독T세포내천공소(Perforin)、간우소(IFN-γ)화백세포개소10(IL-10)표체여병독소제적관계.방법 대50례만성을형간염환자외주혈진행단기배양후,이용류식세포술측정CD8~+세포내Perforin、IFN-γ화IL-10표체,병여30례정상대조진행비교;이용형광정량PCR검측만성을형간염환자HBV DNA적재량,분석기여CD8~+세포내Perforin、IFN-γ화IL-10표체적관계.결과 만성을형간염조CD8~+T세포Perforin、IFN-γ화IL-10적표체분별위(5.30 ±2.62)%、(4.05±2.25)%화(0.77 ±0.50)%,명현저우정상대조조(t분별위4.50、4.56、4.20,P균<0.01);26례HBeAg양성적만성을형간염환자CD8~+T세포Pefforin화IFN-γ적표체분별위(4.54 ±1.93)%화(3.32 ±1.59)%,명현저우24례HBeAg음성적만성을형간염환자(t분별위2.22、2.54,P균<0.05);만성을형간염환자CD8~+T세포내Perforin화IFN-γ표체솔여을간병독DNA재량성부상관(r분별위-0.539、-0.340,P<0.01、P<0.05).결론 만성을형간염환자세포독T세포내Perforin、IFN-γ화IL-10표체감소,가능여만성을형간염환자병독장기존재,병정천연불유유관.
Objective To analyze the relationship between cytotoxic T cells Perforin level, IFN-γand IL-10 in patients with chronic hepatitis B. Methods After a short-term cultivation of peripheral blood collected from 50 patients with chronic hepatitis B, a flow cytometry was employed to detect the levels of Per-forin, IFN-γ and IL-10 in CD8~+ cells to compare with those of the health donors the HBV DNA copies in their blood were also measured by RT-PCR to analyze the relationship with Perforin, IFN-γ, and IL-10 in CD8~+ cells. Results In patients with chronic hepatitis B, the levels of Perforin, IFN-γ and IL-10 in CD8~+ ceils were (5.30 ± 2.62)%, (4.05 ± 2.25) % and (0.77 ± 0.50) %, respectively, which were statistical-ly lower than health donors(the t values were 4.50, 4.56 and 4.20 respectively; P < 0.01) ; Of 26 cases of chronic hepatitis B patients with HBeAg-positive Pefforin and IFN-γ in CD8~+ T cells were (4.54 ± 1.93) % and (3.32 ± 1.59)%, respectively, significantly lower than those of the 24 chronic hepatitis B patients with HBeAg-negative (the t values were 2.22 and 2.54, respectively; P <0.05) ; And the levels of Perforin, IFN-γ had a negative relation with the HBV DNA copies (the coefficient correlations-0. 539 and-0. 340; P < 0.01 and P < 0.05). Conclusion In patients with chronic hepatitis B, the reduced levels of Pefforin, IFN-γ and IL-10 may be related with the long-term existence of HBV, and the protracted course of disease.