CAJ | 학술논문

目的探讨RGD多肽接枝聚/β-TCP/PLA复合神经导管桥接周围神经缺损的治疗效果.方法 45只雄性成年Wister大鼠,随机分为3组,每组15只.切断右侧坐骨神经形成10 mm缺损,A组采用单纯PLA神经导管桥接缺损,B组采用RGD多肽接枝聚/β-TCP/PLA复合神经导管桥接缺损,C组采用自体神经移植.术后12周进行大体观察、电生理、小腿三头肌恢复率、组织学、超微结构等测定.结果 B组运动神经传导速度和肌肉湿重恢复率明显优于A组,差异有统计学意义(P<0.05);B组与C组相近,差异无统计学意义(P>0.05).组织学、超微结构测定发现B、C组神经再生情况明显优于A组.结论在坐骨神经损伤修复中,RGD多肽接枝聚/β-TCP/PLA复合神经导管桥接修复效果与自体神经移植相近,可作为一种较理想的神经缺损修复材料.
목적 탐토RGD다태접지취/β-TCP/PLA복합신경도관교접주위신경결손적치료효과.방법 45지웅성성년Wister대서,수궤분위3조,매조15지.절단우측좌골신경형성10 mm결손,A조채용단순PLA신경도관교접결손,B조채용RGD다태접지취/β-TCP/PLA복합신경도관교접결손,C조채용자체신경이식.술후12주진행대체관찰、전생리、소퇴삼두기회복솔、조직학、초미결구등측정.결과 B조운동신경전도속도화기육습중회복솔명현우우A조,차이유통계학의의(P<0.05);B조여C조상근,차이무통계학의의(P>0.05).조직학、초미결구측정발현B、C조신경재생정황명현우우A조.결론 재좌골신경손상수복중,RGD다태접지취/β-TCP/PLA복합신경도관교접수복효과여자체신경이식상근,가작위일충교이상적신경결손수복재료.
Objective To investigate the effect of RGD peptide conjugated poly[ LA-(Glc-Lys) ]/βTCP/PLA nerve conduit for bridging peripheral nerve regeneration defect. Methods Forty-five male Wister rots were randomly divided into 3 groups, with 15 rats each. A 10 mm defect was created in the right sciatic nerve. In group A the gap was bridged by PLA tube. In group B RGD peptide conjugated poly[ LA-(Glc-Lys) ]/β-TCP/PLA nerve conduit was used to repair the defect. Autologous nerve graft was done in group C which served as control. Twelve weeks postoperatively nerve regeneration was evaluated by gross observation,electrophysiology, muscle weight and muscle morphometry of triceps surae, and ultrastructure of the regenerating nerve. Results Twelve weeks after the operation, nerve conduction velocity and muscle weight recovery of group B were better than those of group A. The differences were statistically significant( P < 0.05). There was no significant difference between group B and group C ( P > 0. 05). The results of histology and ultrastructure showed that nerve regeneration in group B and group C was significantly superior to that in group A.Conclusion RGD peptide conjugated poly[LA-(Glc-Lys)]/β-TCP/PLA conduit can achieve similar results in repairing sciatic nerve defect to that of autogentic nerve graft. It may be an ideal material to repair nerve defect.