CAJ | 학술논문

目的用蛋白质二维凝胶电泳图谱,结合质谱技术鉴定、分析化学低氧模拟剂氯化钴( CoCl2)作用人脐带间充质干细胞(MSC)前后蛋白质组的差异表达.方法 CoCl2作用人脐带MSC,应用双向凝胶电泳技术(2-DE)分离细胞总蛋白.ImageMaster 2D Platinum软件分析差异蛋白质点,用基质辅助激光解析串联飞行时间质谱对差异表达蛋白做质谱鉴定,对差异蛋白进行功能分类.结果建立了CoCl2作用人脐带MSC的蛋白质组图谱,鉴定出26个差异表达蛋白,其中11个表达上调,15个表达下调.其生物学功能涉及糖代谢、蛋白质代谢和修饰、脂类代谢、辅酶与辅基、细胞周期、免疫和防御、细胞结构和运动、信号转导、蛋白靶向和定位、神经细胞的活动、肌肉收缩等.其中过氧化物还原酶-1(Prdx1)表达下调,α-烯醇化酶(ENO1)、单胺囊泡转运蛋白1(VAT1)表达上调.结论低氧对人脐带MSC的影响涉及多蛋白及多个功能通路.
목적 용단백질이유응효전영도보,결합질보기술감정、분석화학저양모의제록화고( CoCl2)작용인제대간충질간세포(MSC)전후단백질조적차이표체.방법 CoCl2작용인제대MSC,응용쌍향응효전영기술(2-DE)분리세포총단백.ImageMaster 2D Platinum연건분석차이단백질점,용기질보조격광해석천련비행시간질보대차이표체단백주질보감정,대차이단백진행공능분류.결과건립료CoCl2작용인제대MSC적단백질조도보,감정출26개차이표체단백,기중11개표체상조,15개표체하조.기생물학공능섭급당대사、단백질대사화수식、지류대사、보매여보기、세포주기、면역화방어、세포결구화운동、신호전도、단백파향화정위、신경세포적활동、기육수축등.기중과양화물환원매-1(Prdx1)표체하조,α-희순화매(ENO1)、단알낭포전운단백1(VAT1)표체상조.결론 저양대인제대MSC적영향섭급다단백급다개공능통로.
Objective To analyze the differential proteomics in human umbilical cord mesenchymal stem cells (MSC) induced by chemical hypoxia-mimetic agent cobalt chloride ( CoCl2 ) by two-dimensional gel electrophoresis (2-DE) and mass-spectrometry.Methods 2-DE was performed to separate proteins from treated and untreated human umbilical cord MSC with CoCl2.2-DE images were analyzed by ImageMaster 2D Platinum software 6.0.The differential expressed proteins was identified by MALDI-TOF-MS.The differential proteins were classified based on their functions.Results 2-DE reference patterns of CoCl2 treated human umbilical cord MSC were established.A total of twenty-six differential proteins were identified,of them eleven proteins were up-regulated and fifteen down-regulated.Their biological functions involved in carbohydrate metabolism,protein metabolism and modification,lipid metabolism,coenzyme and prosthetic group metabolism,cell cycle,immunity and defense,cell structure and motility,signal transduction,protein targeting and localization,neuronal activities,muscle contraction,etc.Peroxiredoxinl (Prdx) was down-regulated,whereas alpha-enolase (ENO1) and vesicle amine transport protein 1 homolog (VAT1) up-regulated.ConclusionThe effects of hypoxia on human umbilical cord MSC were participated by multiple proteins and involved in multiple functional pathways.