肿瘤研究与临床
腫瘤研究與臨床
종류연구여림상
CANCER RESEARCH AND CLINIC
2012年
6期
373-375
,共3页
庞文广%巫国勇%叶敏%伍硕允
龐文廣%巫國勇%葉敏%伍碩允
방문엄%무국용%협민%오석윤
癌,非小细胞肺%血管内皮生长因子%nm23%免疫组织化学
癌,非小細胞肺%血管內皮生長因子%nm23%免疫組織化學
암,비소세포폐%혈관내피생장인자%nm23%면역조직화학
Carcinoma,non-small-cell lung%Vascular endothelial growth foctor%nm 23%Immunohistochemistry
目的 研究血管内皮生长因子(VEGF)和nm 23在非小细胞肺癌(NSCLC)组织中的表达及相互关系,探讨其在NSCLC诊治中的临床意义.方法 采用免疫组织化学链酶素抗生物素蛋白-过氧化物酶连结(SP)法检测60例NSCLC及癌旁正常肺组织中VEGF和nm 23的表达,结合临床病理因素进行分析.结果 VEGF在NSCLC组织中的阳性表达率(63.3％,38/60)显著高于癌旁肺组织(16.7％,10/60)(x2=27.22,P＜0.01).nm 23在NSCLC组织中的阳性表达率(53.3％,32/60)则显著低于癌旁肺组织中的表达(88.3％,53/60)(x2=17.79,P＜0.01).VEGF的阳性率与淋巴结转移及组织学分级呈正相关(x 2=15.23、x2=7.25,P＜ 0.05).nm 23与淋巴结转移呈负相关(x2=16.93,P＜ 0.01).VEGF阳性患者2年生存率明显低于阴性患者(x2=5.55,P＜0.05),nm 23则相反(x 2=12.86,P＜0.05).VEGF与nm 23在NSCLC中表达无相关性(x2=1.83,P＞ 0.05).结论 VEGF和nm 23可能与NSCLC的发生、发展有关,检测VEGF和nm 23可能有助于对NSCLC生物学行为的判断,对肺癌患者的诊治和预后评估有积极意义.
目的 研究血管內皮生長因子(VEGF)和nm 23在非小細胞肺癌(NSCLC)組織中的錶達及相互關繫,探討其在NSCLC診治中的臨床意義.方法 採用免疫組織化學鏈酶素抗生物素蛋白-過氧化物酶連結(SP)法檢測60例NSCLC及癌徬正常肺組織中VEGF和nm 23的錶達,結閤臨床病理因素進行分析.結果 VEGF在NSCLC組織中的暘性錶達率(63.3％,38/60)顯著高于癌徬肺組織(16.7％,10/60)(x2=27.22,P＜0.01).nm 23在NSCLC組織中的暘性錶達率(53.3％,32/60)則顯著低于癌徬肺組織中的錶達(88.3％,53/60)(x2=17.79,P＜0.01).VEGF的暘性率與淋巴結轉移及組織學分級呈正相關(x 2=15.23、x2=7.25,P＜ 0.05).nm 23與淋巴結轉移呈負相關(x2=16.93,P＜ 0.01).VEGF暘性患者2年生存率明顯低于陰性患者(x2=5.55,P＜0.05),nm 23則相反(x 2=12.86,P＜0.05).VEGF與nm 23在NSCLC中錶達無相關性(x2=1.83,P＞ 0.05).結論 VEGF和nm 23可能與NSCLC的髮生、髮展有關,檢測VEGF和nm 23可能有助于對NSCLC生物學行為的判斷,對肺癌患者的診治和預後評估有積極意義.
목적 연구혈관내피생장인자(VEGF)화nm 23재비소세포폐암(NSCLC)조직중적표체급상호관계,탐토기재NSCLC진치중적림상의의.방법 채용면역조직화학련매소항생물소단백-과양화물매련결(SP)법검측60례NSCLC급암방정상폐조직중VEGF화nm 23적표체,결합림상병리인소진행분석.결과 VEGF재NSCLC조직중적양성표체솔(63.3％,38/60)현저고우암방폐조직(16.7％,10/60)(x2=27.22,P＜0.01).nm 23재NSCLC조직중적양성표체솔(53.3％,32/60)칙현저저우암방폐조직중적표체(88.3％,53/60)(x2=17.79,P＜0.01).VEGF적양성솔여림파결전이급조직학분급정정상관(x 2=15.23、x2=7.25,P＜ 0.05).nm 23여림파결전이정부상관(x2=16.93,P＜ 0.01).VEGF양성환자2년생존솔명현저우음성환자(x2=5.55,P＜0.05),nm 23칙상반(x 2=12.86,P＜0.05).VEGF여nm 23재NSCLC중표체무상관성(x2=1.83,P＞ 0.05).결론 VEGF화nm 23가능여NSCLC적발생、발전유관,검측VEGF화nm 23가능유조우대NSCLC생물학행위적판단,대폐암환자적진치화예후평고유적겁의의.
Objective To detect the expressions of VEGF and nm23 in non-small cell lung cancer (NSCLC) tissues and their relationships,and explore their clinical value in the diagnosis and treatment.Methods lmmunohistochemistry(SP method) was used to detect the expressions of VEGF and nm 23 in NSCLC and peritumor tissues.Results The positive expression rate of VEGF in NSCLC(63.3 ％,38/60)was significantly higher than in the peritumor tissues(16.7 ％,10/60)(x2=27.22,P＜0.01).nm 23 is on the contrary (53.3 ％,32/60)(x2=17.79,P＜0.01).The positive expression of VEGF in NSCLC was positively correlated significantly with the lymph node metastasis and histological classification(P＜0.05).The positive expression of nm 23 in NSCLC was negative correlated significantly with lymph node metastasis,P＜0.01.2-year survival rate of the VEGF-positive patients was significantly lower than that of VEGF-negative patients,and nm 23 is on the contrary(x2=5.55,P＜0.05).No correlation was found between the expressions of VEGF and nm 23 in NSCLC (x2=1.83,P＞0.05) Conclusion The VEGF and nm 23 may be closely associated to carcinogenesis and progression of NSCLC.Detection of VEGF and nm 23 are beneficial to indicate the biologic behavior of NSCLC.It has positive effect on diagnosis and treatment of NSCLC.
