CAJ | 학술논문

采用沸水回流法从赤灵芝子实体中提取多糖,经Sevage法除蛋白,乙醇沉淀,离心、流水透析、浓缩、冻干后得灵芝多糖,单糖经乙酰化处理进行外标法定量,并利用苯酚-硫酸法、紫外、红外及X衍射光谱法、凝胶分子排阻色谱-蒸发散射检测器法、气相和气质谱色谱法进行多糖组分、含量、结构和分子最分析研究,结果表明:灵芝多糖为米黄色,得率为2％左右,其含量≥43％,红外光谱显示灵芝多糖结构主要为甘键连接的吡喃型葡聚糖.其多糖的主要单糖组分为葡萄糖,含量为89％左右,并含有其他少量的单糖组分D-阿拉伯糖、D-木糖、D-甘露糖、D-半乳糖.其多糖主要为同均糖,多糖为非晶型结构,分子量主要分布在8×10~4～2×10~5之间,分子质量主要为2×10~5的生物大分子.
채용비수회류법종적령지자실체중제취다당,경Sevage법제단백,을순침정,리심、류수투석、농축、동간후득령지다당,단당경을선화처리진행외표법정량,병이용분분-류산법、자외、홍외급X연사광보법、응효분자배조색보-증발산사검측기법、기상화기질보색보법진행다당조분、함량、결구화분자최분석연구,결과표명:령지다당위미황색,득솔위2％좌우,기함량≥43％,홍외광보현시령지다당결구주요위감건련접적필남형포취당.기다당적주요단당조분위포도당,함량위89％좌우,병함유기타소량적단당조분D-아랍백당、D-목당、D-감로당、D-반유당.기다당주요위동균당,다당위비정형결구,분자량주요분포재8×10~4～2×10~5지간,분자질량주요위2×10~5적생물대분자.
Ganoderma lucidum polysaccharides were extracted by boiling water from the fruiting bodies of red ganoderma lucidum, then protein was removed by Sevage reagent, with ethanol precipitation, run water dialysis, centrifugation and freeing-dr-ying. Monosaccharides were acetylated and quantified by external standard, and the polysaccharides the amount of total carbohydrates was determined by phenol-sulfuric acid method. The monosaccharide constituents, structure and Mw distribution were analyzed by UV-Vis, IR, GC, GC-MS and HPSEC with ELSD. The results demonstrated that ganoderma lucidum polysaccharides were beige, the yield was about 2%, and the content ≥43%. IR spectrometry showed that the structure of ganoderma lucidum polysaccharide was pyranoid glucan withIt was a homogeneous polysaccharide with the amorphous structure, attributed to biological macromolecules, its Mw distribution was at 80-2 000 Kda, and a primary Mw at 2 000 Kda.