CAJ | 학술논문

采用双向电泳技术,对浙江乐清无花纹和有花纹文蛤外套膜的全蛋白进行了研究,并利用质谱和软件对差异蛋白进行了分析.经PDQuest软件分析,在pH 4-7,分子量14.3-200kD之间,无花纹文蛤样品中检测到682±24个蛋白质点,有花纹样品检测到600±35个蛋白质点,平均匹配率为84.5%.研究结果显示,两种文蛤共有256个点存在差异表达,这些差异点可能与贝壳的颜色和花纹形成有关.其中98个点为无花纹文蛤特有,57个点为有花纹文蛤特有,另有101个点在表达量上存在两倍以上差异.在无花纹贝壳中发现分子量为42kD左右,等电点为6.4左右,与库蚊的肌动蛋白(分子量为41.7kD,等电点为5.3)匹配率较高;分子量为29kD,等电点为5.6,与飞鱿的肌动蛋白(分子量为29KD,等电点为5.25)匹配率较高的两个蛋白质.在有花纹文蛤中发现了分子量为27kD左右,等电点为4.8,与丽文蛤属的肌浆钙结合蛋白(分子量为20.8kD,等电点为4.98)匹配率较高.
채용쌍향전영기술,대절강악청무화문화유화문문합외투막적전단백진행료연구,병이용질보화연건대차이단백진행료분석.경PDQuest연건분석,재pH 4-7,분자량14.3-200kD지간,무화문문합양품중검측도682±24개단백질점,유화문양품검측도600±35개단백질점,평균필배솔위84.5%.연구결과현시,량충문합공유256개점존재차이표체,저사차이점가능여패각적안색화화문형성유관.기중98개점위무화문문합특유,57개점위유화문문합특유,령유101개점재표체량상존재량배이상차이.재무화문패각중발현분자량위42kD좌우,등전점위6.4좌우,여고문적기동단백(분자량위41.7kD,등전점위5.3)필배솔교고;분자량위29kD,등전점위5.6,여비우적기동단백(분자량위29KD,등전점위5.25)필배솔교고적량개단백질.재유화문문합중발현료분자량위27kD좌우,등전점위4.8,여려문합속적기장개결합단백(분자량위20.8kD,등전점위4.98)필배솔교고.
Using two-dimensional gel electrophoresis, the protein of whole mantle of patterned and non-patterned Meretrix meretrix, from Yueqing, Zhejiang, East China, was studied in mass spectrometry. With software PDQuest, in pH 4-7 and molecular weight between 14.3-200kD, 682±24 protein spots were detected in the non-pattern clams, and for patterns ones, being 600± 35.The average matching rate was 84.5%. Different expressions were shown in 256 spots, being probably related to the shell pattern formation, among them 98 were from non-patterned clams, and 57 from the patterned. In addition, expression of over twice as much difference was revealed in 101 spots. Two proteins of high rate were found. One was about 42kD in the patterned clams with isoelectric point at about 6.4, matching Culex actin (molecular weight 41.7kD, isoelectric point of 5.3); another was 29kD with isoelectric point 5.6 matching flying squid actin protein (molecu-lar weight of 29kD, isoelectric point of 5.25). For the non-patterned clams, a 27kD molecular weighted one was found with isoelectric point of 4.8, matching well sarcoplasmic calcium-binding protein (molecular weight 20.8kD, isoelectric point of 4.98) in genus Meretrix lusoria.