中国人兽共患病学报
中國人獸共患病學報
중국인수공환병학보
CHINESE JOURNAL OF ZOONOSES
2010年
2期
158-162
,共5页
修文琼%刘光华%康玉兰%沈晓娜%谢剑锋%王美爱%张文清%郑奎城
脩文瓊%劉光華%康玉蘭%瀋曉娜%謝劍鋒%王美愛%張文清%鄭奎城
수문경%류광화%강옥란%침효나%사검봉%왕미애%장문청%정규성
人博卡病毒%全基因组%序列测定%种系分析
人博卡病毒%全基因組%序列測定%種繫分析
인박잡병독%전기인조%서렬측정%충계분석
human bocavirus%complete genome%sequencing%phylogenetic analysis
目的 了解福州地区人博卡病毒(HBoV)在儿童呼吸道感染中的检出情况,并对其进行全基因组序列测定和种系分析.方法 收集2007年11月至2008年10月在福建省妇幼保健院因下呼吸道感染住院的重症监护病房的57例小儿鼻咽抽取物标本,用一对特异引物通过PCR扩增法对HBoV基因片段进行检测,对检测出的2例HBoV(FZ1和FZ40)用7对全序列引物进行扩增和拼接,获得这两株病毒全基因组序列,上传GenBank并与基因库中国内外其它10株HBoV的全基因组序列和各氨基酸序列进行比对,并做种系分析.结果 FZ1株基因组序列全长为5 299bp,与HBoV参考株st2株序列长度相同;而FZ40株的基因组序列全长少2bp.病毒全基因组编码4种蛋白,分别是非结构蛋白NS1、核蛋白NP-1和衣壳蛋白VP1、VP2.结论 种系分析显示福州的FZ40株与浙江温岭的WLL-1株关系较近,而FZ1株与北京的两株及泰国的CU6株关系较近.
目的 瞭解福州地區人博卡病毒(HBoV)在兒童呼吸道感染中的檢齣情況,併對其進行全基因組序列測定和種繫分析.方法 收集2007年11月至2008年10月在福建省婦幼保健院因下呼吸道感染住院的重癥鑑護病房的57例小兒鼻嚥抽取物標本,用一對特異引物通過PCR擴增法對HBoV基因片段進行檢測,對檢測齣的2例HBoV(FZ1和FZ40)用7對全序列引物進行擴增和拼接,穫得這兩株病毒全基因組序列,上傳GenBank併與基因庫中國內外其它10株HBoV的全基因組序列和各氨基痠序列進行比對,併做種繫分析.結果 FZ1株基因組序列全長為5 299bp,與HBoV參攷株st2株序列長度相同;而FZ40株的基因組序列全長少2bp.病毒全基因組編碼4種蛋白,分彆是非結構蛋白NS1、覈蛋白NP-1和衣殼蛋白VP1、VP2.結論 種繫分析顯示福州的FZ40株與浙江溫嶺的WLL-1株關繫較近,而FZ1株與北京的兩株及泰國的CU6株關繫較近.
목적 료해복주지구인박잡병독(HBoV)재인동호흡도감염중적검출정황,병대기진행전기인조서렬측정화충계분석.방법 수집2007년11월지2008년10월재복건성부유보건원인하호흡도감염주원적중증감호병방적57례소인비인추취물표본,용일대특이인물통과PCR확증법대HBoV기인편단진행검측,대검측출적2례HBoV(FZ1화FZ40)용7대전서렬인물진행확증화병접,획득저량주병독전기인조서렬,상전GenBank병여기인고중국내외기타10주HBoV적전기인조서렬화각안기산서렬진행비대,병주충계분석.결과 FZ1주기인조서렬전장위5 299bp,여HBoV삼고주st2주서렬장도상동;이FZ40주적기인조서렬전장소2bp.병독전기인조편마4충단백,분별시비결구단백NS1、핵단백NP-1화의각단백VP1、VP2.결론 충계분석현시복주적FZ40주여절강온령적WLL-1주관계교근,이FZ1주여북경적량주급태국적CU6주관계교근.
The human bocavirus (HBoV) most likely the second known parvovirus species pathogenic to humans was investigated. In this study, the presence of HBoV in children with respiratory tract disease in Fuzhou, Fujian, China was detected. Nasopharyngeal aspirates were collected from children with respiratory tract disease from Nov. 2007 to Oct. 2008.-A total of 57 clinical samples were tested for HBoV using PCR assay. The complete genome of the two HBoV isolates (FZ1, FZ40) were sequenced and deposited in GenBank (Acession Nos. GQ455987, GQ455988).-It was demonstrated that the length of two sequences of the HBoV isolates in this study varied each other, and the sequence length of strain FZ1 in nucleotides was 5 299bp, which was 2bp longer than that of FZ40. The deleted base pair located at site 5 286 in the end of the non-coding region. The complete genome of the HBoV encoding 4 proteins and they were non-structural protein NS1, nuclear protein NP1 and two capsid proteins VP1, VP2.-We had compared the complete genome sequence of the two HBoV isolates in Fuzhou, China to other HBoV isolates and other virus of Parvovirinae documented in the GenBank database by using PHYLIP software version 3.65 and the neighbor-joining method.-The result of phylogenetic analysis showed that the 2 HBoV strains in our study were not clustered together and strain FZ40 was more closed to the WLL-1 strain of China than strain FZ1. Meanwhile, the strain FZ1 was more closed to the two strains of Beijing and CU6 strain of Thailand.