CAJ | 학술논문

目的:研究不同处理方法对卵白蛋白(Ovum Albumin,OVA)变应性鼻炎小鼠模型腹腔肥大细胞脱颗粒的影响.方法:将60只雌性小鼠随机分为5组,每组12只,分别按不同方法处理后分离各组小鼠的腹腔肥大细胞,中性红染色后计算腹脏肥大细胞脱颗粒率.结果:正常对照组、OVA变应性鼻炎组、穴位敷贴组、激素对照组、磷酸盐缓冲液(Phosphate Buffer Saline,PBS)阴性对照组小鼠腹腔肥大细胞脱颗粒率分别为(15±6)%、(53±11)%、(37±13)%、(31±15)%、(47±14)%.OVA变应性鼻炎小鼠的腹腔肥大细胞有明显的脱颗粒现象;与OVA变应性鼻炎小鼠相比,穴位敷贴组及激素对照组小鼠的腹腔肥大细胞脱颗粒现象显著减轻;PBS对照组小鼠的腹腔肥大细胞脱颗粒现象没有明显变化.结论:推测穴位敷贴抗过敏机制为稳定肥大细胞膜,抑制肥大细胞脱颗粒,减少致炎介质产生.
목적:연구불동처리방법대란백단백(Ovum Albumin,OVA)변응성비염소서모형복강비대세포탈과립적영향.방법:장60지자성소서수궤분위5조,매조12지,분별안불동방법처리후분리각조소서적복강비대세포,중성홍염색후계산복장비대세포탈과립솔.결과:정상대조조、OVA변응성비염조、혈위부첩조、격소대조조、린산염완충액(Phosphate Buffer Saline,PBS)음성대조조소서복강비대세포탈과립솔분별위(15±6)%、(53±11)%、(37±13)%、(31±15)%、(47±14)%.OVA변응성비염소서적복강비대세포유명현적탈과립현상;여OVA변응성비염소서상비,혈위부첩조급격소대조조소서적복강비대세포탈과립현상현저감경;PBS대조조소서적복강비대세포탈과립현상몰유명현변화.결론:추측혈위부첩항과민궤제위은정비대세포막,억제비대세포탈과립,감소치염개질산생.
Objective:To investigate effects of different treatment on celiac mast cell degranulation in mice with allergic rhinitis induced with ovum albumin (OVA).Methods:60 female mice were randomly divided into five groups,12 mice in each,which were treated with different methods.Then,the celiac mast cells were separated and degranulation rate was calculated after stained with neutral red.Results:the rates of mast cell degranulation were (15±6)%,(53±11)%,(37±13)%,(31±15)%,and (47±14)% in normal group,OVA group,point application group,hormone group,and PBS group.There is obvious degranulation in the celiac mast cells of mice with OVA-induced allergic rhinitis.Point application and dexamethasone treatment could relieve the mast cell degranulation,whereas,PBS has no effect on the mast cell degranulation.Conclusion:the mechanism of antianaphylaxis of point application may lie on stabilizing the mast cell membrane,and inhibiting degranulation to reduce the inflammatory mediator.