国际呼吸杂志
國際呼吸雜誌
국제호흡잡지
INTERNATIONAL JOURNAL OF RESPIRATION
2012年
10期
745-748
,共4页
机械牵拉%A549%p38%信号转导
機械牽拉%A549%p38%信號轉導
궤계견랍%A549%p38%신호전도
Mechanical stretch%A549%p38%Signal transduction
目的 探讨p38蛋白激酶(p38 MAPK)是否参与周期性机械牵拉刺激在人肺泡Ⅱ型上皮A549细胞的信号转导过程.方法 实验分对照组(刺激前)、机械牵拉(刺激后)5、15、30、60 min共5组,每组3皿细胞.建立离体的A549细胞机械牵拉模型:以大小为1000 μstrain的牵拉力刺激细胞,在刺激前和刺激后5、15、30、60 min时间点进行观察.结果 A549细胞经机械牵拉刺激后,磷酸化p38蛋白水平增加,以30 min时间点最为明显.随着机械牵拉时间的延长,在60 min时间点磷酸化p38蛋白水平下降至约基线水平.结论 机械牵拉刺激信号可激活A549细胞p38 MAPK,从而引起继发细胞信号转导及功能改变.
目的 探討p38蛋白激酶(p38 MAPK)是否參與週期性機械牽拉刺激在人肺泡Ⅱ型上皮A549細胞的信號轉導過程.方法 實驗分對照組(刺激前)、機械牽拉(刺激後)5、15、30、60 min共5組,每組3皿細胞.建立離體的A549細胞機械牽拉模型:以大小為1000 μstrain的牽拉力刺激細胞,在刺激前和刺激後5、15、30、60 min時間點進行觀察.結果 A549細胞經機械牽拉刺激後,燐痠化p38蛋白水平增加,以30 min時間點最為明顯.隨著機械牽拉時間的延長,在60 min時間點燐痠化p38蛋白水平下降至約基線水平.結論 機械牽拉刺激信號可激活A549細胞p38 MAPK,從而引起繼髮細胞信號轉導及功能改變.
목적 탐토p38단백격매(p38 MAPK)시부삼여주기성궤계견랍자격재인폐포Ⅱ형상피A549세포적신호전도과정.방법 실험분대조조(자격전)、궤계견랍(자격후)5、15、30、60 min공5조,매조3명세포.건립리체적A549세포궤계견랍모형:이대소위1000 μstrain적견랍력자격세포,재자격전화자격후5、15、30、60 min시간점진행관찰.결과 A549세포경궤계견랍자격후,린산화p38단백수평증가,이30 min시간점최위명현.수착궤계견랍시간적연장,재60 min시간점린산화p38단백수평하강지약기선수평.결론 궤계견랍자격신호가격활A549세포p38 MAPK,종이인기계발세포신호전도급공능개변.
Objective To explore whether p38 MAPK is involved in the mechanotransduction of A549 cells induced by cyclic mechanical stretch.Methods Cells were divided into five groups:stretch 5,15,30,60 min groups and control group.Three replicate wells were used in each group.Mechanical stretch model in vitro cultured A549 cells was established for the study.Cells were stimulated by stretch at the magnitude of 1000 μstrain for 5,15,30,60 min.Then the protein level of p38 was tested by Western blot.Results After mechanical stimulation,the protein level of phosphorylated p38 MAPK increased,which was most obviously at 30 min time point.However,the protein level of phosphorylated p38 MAPK returned to the original level at 60 min time point.Conclusions Mechanical stretch activates the expression of p38 in A549 cells,leading to secondary signal transduction and function change.
