中华老年医学杂志
中華老年醫學雜誌
중화노년의학잡지
Chinese Journal of Geriatrics
2012年
9期
778-781
,共4页
阿迪拉·多力坤%吐尔逊·买买提%佐热古力·热依木%哈木拉提·吾甫尔%多力坤·买买提玉素甫%郑秀芬
阿迪拉·多力坤%吐爾遜·買買提%佐熱古力·熱依木%哈木拉提·吾甫爾%多力坤·買買提玉素甫%鄭秀芬
아적랍·다력곤%토이손·매매제%좌열고력·열의목%합목랍제·오보이%다력곤·매매제옥소보%정수분
糖尿病,2型%脂蛋白脂酶%多态性,单核苷酸%汉族
糖尿病,2型%脂蛋白脂酶%多態性,單覈苷痠%漢族
당뇨병,2형%지단백지매%다태성,단핵감산%한족
Diabetes mellitus,type 2%Lipoprotein lipase%Polymorphism,single nucleotide%Han nationality
目的 探讨脂蛋白脂酶(LPL)基因HindⅢ和PvuⅡ位点多态性与2型糖尿病(T2DM)的相关性. 方法 采用限制性片段长度多态性聚合酶链反应(PCR RFLP)法分析110例T2DM患者和157例正常人LPL基因HindⅢ和PvuⅡ多态位点的基因型. 结果 LPL基因HindⅢ位点3种基因型H—H-、H+H-、H+H+和2种等位基因H-、H+分布频率在两组间比较差异无统计学意义(x2=1.998,1.262,P=0.368,0.261). LPL基因PvuⅡ位点3种基因型P-P(20.9％比10.8％)、P+P-(40.0％比58.6％)、P+P+(39.1％比30.6％)分布频率在病例组与对照组比较差异有统计学意义(x2=10.157,P=0.006).在PvuⅡ位点携带P+P-基因型的个体较携带p-p-基因型的个体患2型糖尿病的危险性增加(OR=2.833,95％CI=1.374～5.814). 结论 LPL基因HindⅢ多态性与新疆汉族人群T2DM的危险性无关,PvuⅡ多态性与新疆汉族人群T2DM的危险性相关,其中P+P-基因型可能是T2DM的易感因素.
目的 探討脂蛋白脂酶(LPL)基因HindⅢ和PvuⅡ位點多態性與2型糖尿病(T2DM)的相關性. 方法 採用限製性片段長度多態性聚閤酶鏈反應(PCR RFLP)法分析110例T2DM患者和157例正常人LPL基因HindⅢ和PvuⅡ多態位點的基因型. 結果 LPL基因HindⅢ位點3種基因型H—H-、H+H-、H+H+和2種等位基因H-、H+分佈頻率在兩組間比較差異無統計學意義(x2=1.998,1.262,P=0.368,0.261). LPL基因PvuⅡ位點3種基因型P-P(20.9％比10.8％)、P+P-(40.0％比58.6％)、P+P+(39.1％比30.6％)分佈頻率在病例組與對照組比較差異有統計學意義(x2=10.157,P=0.006).在PvuⅡ位點攜帶P+P-基因型的箇體較攜帶p-p-基因型的箇體患2型糖尿病的危險性增加(OR=2.833,95％CI=1.374～5.814). 結論 LPL基因HindⅢ多態性與新疆漢族人群T2DM的危險性無關,PvuⅡ多態性與新疆漢族人群T2DM的危險性相關,其中P+P-基因型可能是T2DM的易感因素.
목적 탐토지단백지매(LPL)기인HindⅢ화PvuⅡ위점다태성여2형당뇨병(T2DM)적상관성. 방법 채용한제성편단장도다태성취합매련반응(PCR RFLP)법분석110례T2DM환자화157례정상인LPL기인HindⅢ화PvuⅡ다태위점적기인형. 결과 LPL기인HindⅢ위점3충기인형H—H-、H+H-、H+H+화2충등위기인H-、H+분포빈솔재량조간비교차이무통계학의의(x2=1.998,1.262,P=0.368,0.261). LPL기인PvuⅡ위점3충기인형P-P(20.9％비10.8％)、P+P-(40.0％비58.6％)、P+P+(39.1％비30.6％)분포빈솔재병례조여대조조비교차이유통계학의의(x2=10.157,P=0.006).재PvuⅡ위점휴대P+P-기인형적개체교휴대p-p-기인형적개체환2형당뇨병적위험성증가(OR=2.833,95％CI=1.374～5.814). 결론 LPL기인HindⅢ다태성여신강한족인군T2DM적위험성무관,PvuⅡ다태성여신강한족인군T2DM적위험성상관,기중P+P-기인형가능시T2DM적역감인소.
Objective To study the relationship between lipoprotein lipase (LPL) gene Hind Ⅲ and Pvu Ⅱ polymorphisms and type 2 diabetes mellitus (T2DM).Methods Polymerase chain reaction-restriction fragment length polymorphism-(PCR RFLP) technique was used to detect the genotypes of Hind Ⅲ and Pvu Ⅱ polymorphisms in the LPL gene from 110 T2DM patients and 157 healthy controls.Results The frequencies ofgenotype (H-H-,H+H-,H+H+)and allele(H,H+) of Hind Ⅲ between T2DM cases and controls showed no differences (x2 =1.998,1.262,P 0.368,0.261).There were differences in the frequency of genotype P-P-(20.9％ vs.10.8％),P+P-(40.0％ vs.58.6％),P+P+(39.1％ vs.30.6％) of PvuⅡ between T2DM and controls (x2 =10.157,P=0.006).The individuals who carried P+P genotype had an higher morbidity of T2DM than those who carried P P genotype (OR 2.833,95％CI:1.374 5.814).Conclusions The Hind Ⅲ polymorphisms in the LPL gene is not associated with T2DM risk,but the Pvu Ⅱ polymorphisms in the LPL gene is related with T2DM risk in Chinese Han population in Xinjiang,and the P+P- genotype might be a susceptible factor of T2DM.
