CAJ | 학술논문

目的通过对自发性2型糖尿病GK大鼠干预治疗,观察并探讨中药复方糖耐康对糖尿病大血管病变的保护作用.方法将51只GK大鼠随机分为模型组、吡格列酮组、中药糖耐康低、中、高剂量组共5组.另设10只Wistar大鼠为正常组.灌胃8周后,观察大鼠精神状态、体重、饮水量、活动情况,每周监测体重变化,每4周监测空腹血糖变化.HE染色观察胸丰动脉病理改变,免疫组化法测定胸主动脉血管内皮生长因子(VEGF)蛋白表达.结果模型组空腹血糖显著升高,24 h饮水量明显增加,体重增长变缓,各治疗组上述情况均有不同程度改善.HE染色高倍镜下,模型组动脉壁内膜增厚,内皮细胞肿胀脱落,内弹力板呈波浪状或交织状排列,有断裂,中膜浅层平滑肌增生、排列紊乱.各治疗组上述病理改变均有不同程度减轻.免疫组化图像分析结果显示,与模型组相比较,各治疗组主动脉VEGF蛋白表达均显著降低(P<0.01).结论中药复方糖耐康可能通过下调GK大鼠胸主动脉VEGF蛋白表达而发挥大血管保护作用.
목적 통과대자발성2형당뇨병GK대서간예치료,관찰병탐토중약복방당내강대당뇨병대혈관병변적보호작용.방법 장51지GK대서수궤분위모형조、필격렬동조、중약당내강저、중、고제량조공5조.령설10지Wistar대서위정상조.관위8주후,관찰대서정신상태、체중、음수량、활동정황,매주감측체중변화,매4주감측공복혈당변화.HE염색관찰흉봉동맥병리개변,면역조화법측정흉주동맥혈관내피생장인자(VEGF)단백표체.결과 모형조공복혈당현저승고,24 h음수량명현증가,체중증장변완,각치료조상술정황균유불동정도개선.HE염색고배경하,모형조동맥벽내막증후,내피세포종창탈락,내탄력판정파랑상혹교직상배렬,유단렬,중막천층평활기증생、배렬문란.각치료조상술병리개변균유불동정도감경.면역조화도상분석결과현시,여모형조상비교,각치료조주동맥VEGF단백표체균현저강저(P<0.01).결론 중약복방당내강가능통과하조GK대서흉주동맥VEGF단백표체이발휘대혈관보호작용.
Objective To investigated the effect of TangNaiKang (TNK) on VEGF protein expression of GK rats Thoracic aorta. Methods 51 male GK rats were divided into five groups randomly: model group, pioglitazone group, and TNK treatment group (low, immediate and high dose). Another 10 male Wistar rats were served as normal control group. GK rats were fed with high-grease forage, while normal control group was fed with a standard diet. Fasting blood glucose, general HE staining and VEGF protein expression were detected by immunohistochemistry. Results The fasting glucose had a significant decline in TNK treatment groups. HE staining showing TNK can ameliorate intima thickness, reduce hyperplasia of shallow vascular smooth muscle cell, and improve wavy and plexiform arrangement of elastic lamina. Immunohistochemistry also showed that TNK decreased VEGF protein expression of great vessels. Conclusion TangNaiKang can prevent and cure diabetic vascular complication of GK rats.