CAJ | 학술논문

　　目的：通过等长收缩法检测小鼠肠系膜动脉的舒缩效应，为研究小鼠阻力血管张力变化及其调控机制提供直观、可靠的实验方法。方法：取C57BL/6成年雄鼠肠系膜动脉制备1.0 mm长血管环，在苯肾上腺素预收缩的基础上，观察乙酰胆碱、花生四烯酸对小鼠肠系膜动脉张力变化的影响。结果：未加入一氧化氮合酶抑制剂，乙酰胆碱能很好地诱导小鼠肠系膜动脉血管环的血管舒张效应，该效应随浓度的增加而增大；而在加入一氧化氮合酶抑制剂的情况下，花生四烯酸亦能很好地诱导小鼠肠系膜动脉血管环浓度依赖性的血管舒张效应。结论：成功建立用等长收缩法测定以肠系膜动脉为代表的小鼠阻力血管舒缩反应的方法。
　　목적：통과등장수축법검측소서장계막동맥적서축효응，위연구소서조력혈관장력변화급기조공궤제제공직관、가고적실험방법。방법：취C57BL/6성년웅서장계막동맥제비1.0 mm장혈관배，재분신상선소예수축적기출상，관찰을선담감、화생사희산대소서장계막동맥장력변화적영향。결과：미가입일양화담합매억제제，을선담감능흔호지유도소서장계막동맥혈관배적혈관서장효응，해효응수농도적증가이증대；이재가입일양화담합매억제제적정황하，화생사희산역능흔호지유도소서장계막동맥혈관배농도의뢰성적혈관서장효응。결론：성공건립용등장수축법측정이장계막동맥위대표적소서조력혈관서축반응적방법。
Objective:To detect vasomotor reaction of mouse mesenteric arteries with isometric force measurement in order to establish a direct and reliable method to study the regulatory mechanisms of vessel function in mouse resistance arteries.Method:Vascular rings(1.0 mm)were prepared using the mesenteric artery of C57BL/6 adult male mice to observe the effects of acetylcholine and arachidonic acid on vasomotor reaction of vessels pre-contracted by phenylephrine.Result:Acetylcholine induced dose-dependent vasodilation of mesenteric arterial rings in the absence of nitric oxide synthase inhibitor.Arachidonic acid also evoked vasodilation mesenteric artery in the presence of nitric oxide synthase inhibitor.Conclusion:A method of isometric force measurement is successfully established to detect vasomotor reaction in mouse resistance vessels such as mesenteric artery.