CAJ | 학술논문

目的观察多壁纳米碳圈(MWCNOs)对大鼠血小板黏附及对实验性血栓的影响.方法采用倒置荧光显微镜和流动小室,观察在不同剪切力(500 s-1、1000 s-1)作用下,20 g/ml MWCNOs对大鼠血小板黏附的影响,每组重复至少3次以上.用化学刺激法,分假手术组、溶剂组和MWCNOs组,每组6～9只大鼠,观察2 mg/kg MWCNOs对25％三氯化铁诱导大鼠颈动脉血栓形成中的血流变化及其单位长度血栓湿重的影响.结果 (1)剪切力为500s-1,MWCNOs组的血小板黏附数量(58.3±16.1 platelets/0.01 mm2)较溶剂组血小板黏附数量(190.1±36.0 platelets/0.01 mm2),差异有统计学意义.当剪切力升高至1000s-1时,MWCNOs对血小板黏附的抑制作用消失.(2)血栓形成试验中,尾静脉注射溶剂及2 mg/kgMWCNOs 0和12h后,0时间点的单位长度血栓湿重为:溶剂组0.83 0.12 mg/mm,MWCNOs组0.970.11 mg/mm,12 h时间点的单位长度血栓湿重为:溶剂组0.89 0.12 mg/mm,MWCNOs组1.01 0.15mg/mm,差异均无统计学意义(P＞0.05).同时,两个时间点溶剂组的血流变化与相应MWCNOs组相比差异无统计学意义(P＞0.05).结论 MWCNOs对离体血小板黏附存在抑制作用,而尾静脉注射MWCNOs对大鼠实验性血栓中的血流及单位长度血栓湿重无影响.
목적 관찰다벽납미탄권(MWCNOs)대대서혈소판점부급대실험성혈전적영향.방법 채용도치형광현미경화류동소실,관찰재불동전절력(500 s-1、1000 s-1)작용하,20 g/ml MWCNOs대대서혈소판점부적영향,매조중복지소3차이상.용화학자격법,분가수술조、용제조화MWCNOs조,매조6～9지대서,관찰2 mg/kg MWCNOs대25％삼록화철유도대서경동맥혈전형성중적혈류변화급기단위장도혈전습중적영향.결과 (1)전절력위500s-1,MWCNOs조적혈소판점부수량(58.3±16.1 platelets/0.01 mm2)교용제조혈소판점부수량(190.1±36.0 platelets/0.01 mm2),차이유통계학의의.당전절력승고지1000s-1시,MWCNOs대혈소판점부적억제작용소실.(2)혈전형성시험중,미정맥주사용제급2 mg/kgMWCNOs 0화12h후,0시간점적단위장도혈전습중위:용제조0.83 0.12 mg/mm,MWCNOs조0.970.11 mg/mm,12 h시간점적단위장도혈전습중위:용제조0.89 0.12 mg/mm,MWCNOs조1.01 0.15mg/mm,차이균무통계학의의(P＞0.05).동시,량개시간점용제조적혈류변화여상응MWCNOs조상비차이무통계학의의(P＞0.05).결론 MWCNOs대리체혈소판점부존재억제작용,이미정맥주사MWCNOs대대서실험성혈전중적혈류급단위장도혈전습중무영향.
Objective To observe the effects of multi-walled carbon nanoonions (MWCNOs) on platelet adhesion and experimental thrombosis in rats.Methods Experimental rats were randomly divided into sham operation group,solvent group,and MWCNO group,each including 6～9 rats.An inverted fluorescence microscope and a flow chamber were used to observe the effects of 20 g/ml MWCNOs on platelet adhesion at shear rates of 500 s-1 and 1000 s-1; the experiment was repeated at least three times in each group.A rat model of carotid artery thrombosis was induced by 25％ FeCl3,and the effects of 2 mg/kg MWCNOs on the blood flow and wet weight of thrombus per millimeter in the model were observed.Results When the shear rate was 500 s-1,the MWCNO group showed a significantly smaller number of adhering platelets than the solvent group (58.3± 16.1 platelets/0.01 mm2 vs 190.1±36.0 platelets/0.01 mm2),but the inhibitory effect of MWCNOs on platelet adhesion disappeared as the shear rate increased to 1000 s-1.The wet weights of thrombus per millimeter at 0 h after injection of a solvent or MWCNOs via the caudal vein were 0.83 ±0.12 mg/mm in the solvent group and 0.97±0.11 mg/mm in the MWCNO group,and the wet weights of thrombus per millimeter at 12 h after injection were 0.89±0.12 mg/mm in the solvent group and 1.01±0.15 mg/mm in the MWCNO group,exhibiting no significant differences between the two groups (P＞0.05).There were also no significant differences between the two groups in terms of blood flow at 0 h and 12 h after injection (P＞0.05).Conclusion MWCNOs have inhibitory effect on platelet adhesion in vitro,but the injection of MWCNOs via the caudal vein has no effects on the blood flow and wet weight of thrombus per millimeter in experimental thrombosis in rats.