CAJ | 학술논문

目的从兰州腹泻和正常儿童粪便标本中检测 Aichi virus,同时探讨 Aichi virus 与婴幼儿腹泻之间的联系.方法根据文献发表资料,采用RT-PCR方法扩增 Aichi virus 3CD 片段,阳性产物经测序确定,并与已发表的该病毒序列进行比对分析.结果在46份腹泻住院患儿粪便标本和299份腹泻门诊就诊患儿标本中各检出1例 Aichi virus,总检出率为0.06%,正常对照儿童中未检测到 Aichi virus.2株病毒3CD区基因与已知参考株核苷酸序列同源性均为97%,系统进化分析显示这2株病毒属于B基因型.结论我国存在B基因型的 Aichi virus,但要明确我国 Aichi virus 的病原学及流行病学特点需要更多研究.
목적 종란주복사화정상인동분편표본중검측 Aichi virus,동시탐토 Aichi virus 여영유인복사지간적련계.방법 근거문헌발표자료,채용RT-PCR방법확증 Aichi virus 3CD 편단,양성산물경측서학정,병여이발표적해병독서렬진행비대분석.결과 재46빈복사주원환인분편표본화299빈복사문진취진환인표본중각검출1례 Aichi virus,총검출솔위0.06%,정상대조인동중미검측도 Aichi virus.2주병독3CD구기인여이지삼고주핵감산서렬동원성균위97%,계통진화분석현시저2주병독속우B기인형.결론 아국존재B기인형적 Aichi virus,단요명학아국 Aichi virus 적병원학급류행병학특점수요경다연구.
Objective To identification and analysis Aichi virus from diarrhea and normal children in Lanzhou, and discuss the relationship between Aichi virus and Infant Diarrhea. Methods According to the literature published data, Using RT-PCR method to amplified Aichi virus 3CD fragment and the positive products were sequenced and determined, and made the alignment analysis between the nucleotide sequences of the amplified fragment with the known sequence of this virus. Results There was 1 case detection of Aichi virus in the 46 hospitalized children with diarrhea and 299 children with diarrhea out-patients specifically, Overall detection rate was 0.06% , and there was no Aichi virus was detected in normal control children. 2 viral 3CD gene and the known reference strains of nucleotide sequences were 97% , while phylogenetic analysis showed that genotype of 2 viral belongs to the B. Conclusions There existed B Genotype of Aichi virus in China, and more research is needed to clarified the etiology and epidemiology of Aichi virus characteristics.