CAJ | 학술논문

目的观察伊马替尼(STI571)体外对急性早幼粒细胞白血病细胞株NB4凋亡的作用.方法不同浓度STI571处理NB4细胞后,分别于不同时相:形态学观察凋亡小体的形成;MTT法检测NB4生长抑制率;用流式细胞仪检测在抗凋亡基因survivin和凋亡基因Smac的表达水平.结果形态学观察到有典型的凋亡小体形成.MTF法检测结果示:NB4生长抑制率上升.流式细胞仪检测结果示:抗凋亡基因survivin的表达水平下降,凋亡基因Smac的表达水平上升,呈时间和剂量依赖性.结论 STI571有潜力诱导NB4细胞发生凋亡.
목적 관찰이마체니(STI571)체외대급성조유립세포백혈병세포주NB4조망적작용.방법 불동농도STI571처리NB4세포후,분별우불동시상:형태학관찰조망소체적형성;MTT법검측NB4생장억제솔;용류식세포의검측재항조망기인survivin화조망기인Smac적표체수평.결과 형태학관찰도유전형적조망소체형성.MTF법검측결과 시:NB4생장억제솔상승.류식세포의검측결과 시:항조망기인survivin적표체수평하강,조망기인Smac적표체수평상승,정시간화제량의뢰성.결론 STI571유잠력유도NB4세포발생조망.
Objective This study was designed to explore the influence about apoptosis of STI571 on NB4.Methods After NB4 cells were treated with STI571 in different concentration(0.5,1.0,5.0umol/L)at indicated time(24h,48h,72h)(1)morphological observation:To determine cell morphology by light microscope.(2)MTT assay:Inhibition of proliferation was measured with a colorimetric 3-(4,5-dimethylthiazol-2-yl)-2,5-dipheny tetrazolium bromide(MYa3 assay.(3)Flow cytometric analysis:The expression of survivin and smac were measured on NB4 by FCM.Results Morphological observation showed characteristic apoptosis changes.MTT assay showed that STI at (0.5,1.0,5.0 μmol/L) range for 24 h,48 h and 72 h can markedly inhibit the proliferation of NB4 cells in a dose-dependent manner as well as a time-dependent manner(P＜0.05).FCM showed the expression of survivin decrease and smac increase.Conclusion STI571 In Vitro inhibits proliferation of NB4.