CAJ | 학술논문

[目的]基因芯片作为高通量、高效率的DNA分析技术,已被广泛地用于植物抗逆性功能基因的分析之中.为探讨盐生植物和甜土植物NHX1基因调控盐应答过程和亲水性C端的功能研究提供借鉴,进一步揭示盐生植物和甜土植物耐盐机制的差异,为植物耐盐机理的实际应用提供依据.[方法]利用Affymetrix拟南芥表达谱芯片对过表达盐生植物灰绿藜和甜土植物水稻NHX1基因及缺失部分C端基因的转基因拟南芥进行基因表达差异的分析.[结果]在盐胁迫的转基因拟南芥组织中,筛选出在四组转基因型拟南芥中共同差异表达的基因有394条,占筛选基因总数的1.64%,其中上调表达的基因有177条,下调表达的基因有217条.将这些差异基因初步分为12类, 包括非生物性与生物性刺激的应答过程、胁迫应答过程、电子转移和能量途径、信号转导和转录等功能类别.[结论]表明植物耐盐过程是一个多基因参与的复杂的变化过程, 涉及到许多相关基因的变化.联系两两比较散点图和聚类分析结果从理论上说明,转基因拟南芥cgNHX1,cgNHX1dc,osNHX1和osNHX1dc耐盐性有依次减弱的趋势.
[목적]기인심편작위고통량、고효솔적DNA분석기술,이피엄범지용우식물항역성공능기인적분석지중.위탐토염생식물화첨토식물NHX1기인조공염응답과정화친수성C단적공능연구제공차감,진일보게시염생식물화첨토식물내염궤제적차이,위식물내염궤리적실제응용제공의거.[방법]이용Affymetrix의남개표체보심편대과표체염생식물회록려화첨토식물수도NHX1기인급결실부분C단기인적전기인의남개진행기인표체차이적분석.[결과]재염협박적전기인의남개조직중,사선출재사조전기인형의남개중공동차이표체적기인유394조,점사선기인총수적1.64%,기중상조표체적기인유177조,하조표체적기인유217조.장저사차이기인초보분위12류, 포괄비생물성여생물성자격적응답과정、협박응답과정、전자전이화능량도경、신호전도화전록등공능유별.[결론]표명식물내염과정시일개다기인삼여적복잡적변화과정, 섭급도허다상관기인적변화.련계량량비교산점도화취류분석결과종이론상설명,전기인의남개cgNHX1,cgNHX1dc,osNHX1화osNHX1dc내염성유의차감약적추세.
[Objective and Method]The gene chip is a DNA analysis technology of high throughput and high efficiency which has been widely used in analysis of resistant function.In this study, using Affymetrix Arabidopsis whole-genome expression gene chip, the differential expression analysis of gene was carried out with the transgenic A. thaliana .[Result]In the transgenic Arabidopsis tissues under salt stress, 394 co-expression genes were selected from the four groups, they accounted for 1.64% of the total number of genes, in which there were 177 up-regulated expression genes and 217 down-regulated expression genes. These genes were initially divided into 12 gene categories, including response to abiotic or biotic stimuli, response to stress , electron transport or energy pathways, signal transduction and transcription, and so on. Our results show that the process of plant salt-tolerant is a multi-gene changed complex process that involves many changes of variety related genes.[Conclusion]The results of comparison scatters and cluster analysis illuminate that the salt tolerance of transgenic Arabidopsis cgNHX1, cgNHX1dc, osNHX1 and osNHX1dc have successively weakened.Our study will contribute to find the differences between halophytic and glycophytic species in the mechanism of plant salt tolerance and the function analysis of hydrophilic C-terminal, provide a favorable evidence for the study of the mechanism of plant salt-tolerance between halophytic and glycophytic species, and offer new methodology in study the mechanism for salt-tolerant plants, provide a basis for practical applications of salt-tolerant mechanism in plants.