CAJ | 학술논문

目的探讨Runx3基因甲基化在儿童恶性淋巴瘤的发生、发展过程中的作用.方法选取确诊为恶性淋巴瘤的患儿48例作为试验组,非恶性肿瘤患儿20例作为对照组,采集患儿骨髓.采用甲基化特异性聚合酶链反应( MS-PCR)法检测骨髓细胞中Runx3基因甲基化状态,用反转录-聚合酶链反应( RT-PCR)法检测mRNA的表达情况.结果 MS-PCR法检测结果显示,试验组治疗前31例出现Runx3基因甲基化条带,阳性率为64.6％,对照组均未检测出,两组比较差异具有统计学意义(x2=15.7,P＜ 0.05).试验组动态观察的42例Runx3甲基化阳性率呈递减趋势.RT-PCR法检测结果显示,对照组20例均有Runx3 mRNA表达,试验组Runx3基因甲基化者均无表达.试验组临床缓解9例中均表达mRNA,复发1例与治疗前一样无表达.结论恶性淋巴瘤患儿骨髓细胞中Runx3基因呈高甲基化状态,使该基因表达受阻,这与儿童恶性淋巴瘤的发生、发展可能相关.
목적 탐토Runx3기인갑기화재인동악성림파류적발생、발전과정중적작용.방법 선취학진위악성림파류적환인48례작위시험조,비악성종류환인20례작위대조조,채집환인골수.채용갑기화특이성취합매련반응( MS-PCR)법검측골수세포중Runx3기인갑기화상태,용반전록-취합매련반응( RT-PCR)법검측mRNA적표체정황.결과 MS-PCR법검측결과현시,시험조치료전31례출현Runx3기인갑기화조대,양성솔위64.6％,대조조균미검측출,량조비교차이구유통계학의의(x2=15.7,P＜ 0.05).시험조동태관찰적42례Runx3갑기화양성솔정체감추세.RT-PCR법검측결과현시,대조조20례균유Runx3 mRNA표체,시험조Runx3기인갑기화자균무표체.시험조림상완해9례중균표체mRNA,복발1례여치료전일양무표체.결론 악성림파류환인골수세포중Runx3기인정고갑기화상태,사해기인표체수조,저여인동악성림파류적발생、발전가능상관.
Objective To study methylation status of the Runx3 gene in occurrence and development of children malignant lymphoma.Methods The bone marrow specimens of 48 children diagnosed as malignant lymphoma were included into experimental group.20 children with non-malignancy were included into control group.Methylation-specific polymerase chain reaction (MS-PCR) was used to detect methylation status of Runx3 gene in bone marrow cells.The reverse transcription-polymerase chain reaction (RT-PCR) was used to detect mRNA expression of Runx3 gene.Results MS-PCR assay results showed that 31 cases expressed Runx3 gene methylation in experimental group,the positive rate was 64.6 ％ (31/48),but no one was detected in control group (20 cases),the difference between two groups was significant (x2 =15.7,P ＜0.05).Dynamic observation of 42 cases in experimental group showed the declining of Runx3 methylation positive rate.RT-PCR assay results showed that all 20 cases in the control group expressed Runx3 gene mRNA,all cases with methylation status of the Runx3 gene in the experimental group didn’ t expressed Runx3 gene mRNA.In experimental group,9 cases of clinical remission expressed Runx3 gene mRNA,and 1 case of relapsed didn’ t expressed Runx3 gene mRNA.Conclusion Runx3 gene shows a high methylation status in bone marrow cells of malignant lymphoma children,so that blocked the expression of Runx3 gene,which is closely related to the occurrence and development of children malignant lymphoma.