中华消化外科杂志
中華消化外科雜誌
중화소화외과잡지
CHINESE JOURNAL OF DIGESTIVE SURGERY
2009年
4期
294-297
,共4页
何渝军%刘宝华%向德兵%张菊馨
何渝軍%劉寶華%嚮德兵%張菊馨
하투군%류보화%향덕병%장국형
大肠肿瘤%咖啡酸苯乙酯%β-连环蛋白
大腸腫瘤%咖啡痠苯乙酯%β-連環蛋白
대장종류%가배산분을지%β-련배단백
Colorectal neoplasms%Caffeic acid phenethyl ester%β-catenin
目的 观察咖啡酸苯乙酯(CAPE)对大肠癌SW480细胞β-连环蛋白相关通路中β-连环蛋白、c-myc和细胞周期蛋白D1基因表达的影响.方法 采用RT-PCR和Western blot方法 检测不同浓度CAPE作用24、48 h后,细胞β-连环蛋白、c-myc及细胞周期蛋白D1 Mrna和蛋白表达的变化.结果 经不同浓度的CAPE作用后,SW480细胞β-连环蛋白、c-myc、细胞周期蛋白D1 Mrna和蛋白表达均有不同程度的下降,分别从1.05±0.26下降到0.67±0.10、0.87±0.09下降到0.51±0.14、0.63±0.09下降到0.32±0.14和204±52下降到52±16、111±11下降到52±16、87±7下降到32±12,与对照组(CAPE浓度为0 mg/L)比较差异有统计学意义(F=5.724,6.793,7.026,15.936,14.889,14.162,31.147,28.881,6.322,17.647,9.584,P<0.05),并呈剂量和时间依赖性.结论 CAPE可干扰β-连环蛋白相关通路,下调β-连环蛋白及通路靶基因c-myc和细胞周期蛋白D1的转录和表达.CAPE抗肿瘤作用可能与下调β-连环蛋白通路相关基因表达有关.
目的 觀察咖啡痠苯乙酯(CAPE)對大腸癌SW480細胞β-連環蛋白相關通路中β-連環蛋白、c-myc和細胞週期蛋白D1基因錶達的影響.方法 採用RT-PCR和Western blot方法 檢測不同濃度CAPE作用24、48 h後,細胞β-連環蛋白、c-myc及細胞週期蛋白D1 Mrna和蛋白錶達的變化.結果 經不同濃度的CAPE作用後,SW480細胞β-連環蛋白、c-myc、細胞週期蛋白D1 Mrna和蛋白錶達均有不同程度的下降,分彆從1.05±0.26下降到0.67±0.10、0.87±0.09下降到0.51±0.14、0.63±0.09下降到0.32±0.14和204±52下降到52±16、111±11下降到52±16、87±7下降到32±12,與對照組(CAPE濃度為0 mg/L)比較差異有統計學意義(F=5.724,6.793,7.026,15.936,14.889,14.162,31.147,28.881,6.322,17.647,9.584,P<0.05),併呈劑量和時間依賴性.結論 CAPE可榦擾β-連環蛋白相關通路,下調β-連環蛋白及通路靶基因c-myc和細胞週期蛋白D1的轉錄和錶達.CAPE抗腫瘤作用可能與下調β-連環蛋白通路相關基因錶達有關.
목적 관찰가배산분을지(CAPE)대대장암SW480세포β-련배단백상관통로중β-련배단백、c-myc화세포주기단백D1기인표체적영향.방법 채용RT-PCR화Western blot방법 검측불동농도CAPE작용24、48 h후,세포β-련배단백、c-myc급세포주기단백D1 Mrna화단백표체적변화.결과 경불동농도적CAPE작용후,SW480세포β-련배단백、c-myc、세포주기단백D1 Mrna화단백표체균유불동정도적하강,분별종1.05±0.26하강도0.67±0.10、0.87±0.09하강도0.51±0.14、0.63±0.09하강도0.32±0.14화204±52하강도52±16、111±11하강도52±16、87±7하강도32±12,여대조조(CAPE농도위0 mg/L)비교차이유통계학의의(F=5.724,6.793,7.026,15.936,14.889,14.162,31.147,28.881,6.322,17.647,9.584,P<0.05),병정제량화시간의뢰성.결론 CAPE가간우β-련배단백상관통로,하조β-련배단백급통로파기인c-myc화세포주기단백D1적전록화표체.CAPE항종류작용가능여하조β-련배단백통로상관기인표체유관.
Objective To study the effects of caffeic acid phenethyl ester (CAPE) on the expression of β-catenin, c-myc and cyclin D1 in colorectal cancer cell line SW480. Methods The changes of mRNA and protein expression of β-catenin, cyclin DI and c-myc were detected by RT-PCR and Western blot after culturing the colorectal cancer cell line SW400 with different concentrations of CAPE (2.5, 5.0, 10.0 mg/L) for 24 hours and 48 hours. Results After the treatment of CAPE, the mRNA expression of β-catenin, cyclin D1 and c-myc were decreased from 1.05±0. 26, 0.87±0.09, 0.63 ± 0. 09 to 0.67 ±0. 10, 0.51±0.14, 0.32±0.14, respectively, and the protein expression of β-catenin, cyclin D1 and c-myc were decreased from 204±52, 111±11, 87±7 to 52±16, 52±16, 32±12, respectively. There was a significant difference in the decrease of mRNA and protein expression of β-catenin, cyclin D1 and c-myc in colorectal cancer cell line SW480 with and without treatment of CAPE (F=5.724, 6.793, 7.026, 15.936, 14.889, 14.162, 31.147, 28.881, 6.322, 17.647, 9.584, P<0.05 ). The inhibition effect of CAPE was displayed in a dose- and time-dependent manner. Conclusions CAPE can obstruct the β-catenin pathway, and down-regulate the transcription and expression of β-catenin, cyclin D1 and c-myc. The anti-tumor effect of CAPE may be related to the decreased expression of β-catenin, cyclin DI and c-myc.
