CAJ | 학술논문

目的动态检测小鼠胆道闭锁(biliary atresia,BA)模型肝脏组织中干扰素-γ(IFN-γ)及其受体IFN-γ R mRNA的表达情况,探索其与BA发生的关系.方法采用腹腔注射恒河猴轮状病毒建立BALB/c新生小鼠BA模型,其巾BA组45只,对照组24只(腹腔注射病毒培养液).应用RT-PCR方法检测小鼠模型肝脏中IFN-γ及其受体mRNA的表达.结果与对照组小鼠相比,不同鼠龄BA组肝内IFN-γ mRNA的表达水平均明显高于对照组,并出现先升高后降低趋势,第九天时达到峰值,约为对照组30倍.IFN-γ受体mRNA表达水平同样出现先升高后下降趋势,第九天达到最高水平,明显高于对照组.IFN-γ受体mRNA在第3、6、9、14天时明显高于对照组,到第21天时恢复到基线水平.结论小鼠BA模型肝组织中IFN-γ及其受体mRNA表达水平基本同步上调,IFN-γ通过受体特异性功能在BA炎症的发生及发展过程中发挥重要作用.
목적 동태검측소서담도폐쇄(biliary atresia,BA)모형간장조직중간우소-γ(IFN-γ)급기수체IFN-γ R mRNA적표체정황,탐색기여BA발생적관계.방법 채용복강주사항하후륜상병독건립BALB/c신생소서BA모형,기건BA조45지,대조조24지(복강주사병독배양액).응용RT-PCR방법검측소서모형간장중IFN-γ급기수체mRNA적표체.결과 여대조조소서상비,불동서령BA조간내IFN-γ mRNA적표체수평균명현고우대조조,병출현선승고후강저추세,제구천시체도봉치,약위대조조30배.IFN-γ수체mRNA표체수평동양출현선승고후하강추세,제구천체도최고수평,명현고우대조조.IFN-γ수체mRNA재제3、6、9、14천시명현고우대조조,도제21천시회복도기선수평.결론 소서BA모형간조직중IFN-γ급기수체mRNA표체수평기본동보상조,IFN-γ통과수체특이성공능재BA염증적발생급발전과정중발휘중요작용.
Objective To study the expression pattern of IFN-γ and IFN-γ receptor in the liver of biliary atresia(BA)mice.Methods Biliary atresia were induced in mice by intra-peritoneal RRV injection.Forty-five mice were of BA group and 24 mice which received 2%FCS-MEM intraperitoneal-ly were used as control The expression of IFN-γ7 and IFN-γ mRNA in livers of mice were detected with RT-PER.Results Compared to the control group,the expression of IFN-γ in the liver of BA mice at different ages was much higher,It increased first and then deereased,peaking on the 9th days after inoculating with RRv.The level was aboUt thirty times that of the control group.Meanwhile the IFN-γ R mRNA expression also increased first and then decreased,and it peaked on the 9th day.It was significantly higher than control group on the 3,6,9,14th day.The level dropped back to that of the control on the 21th day.Conclusions The expression of IFN-γ and its receptor increased almost synchronously in the livers of BA mice,a possible explanation could be receptor specific effects of in-terferon-γ on the signal transduction levels.