中华病理学杂志
中華病理學雜誌
중화병이학잡지
Chinese Journal of Pathology
2009年
7期
441-444
,共4页
吕新全%Zhenhe Suo%马长路%徐珂佳%刘羿杉%李惠翔
呂新全%Zhenhe Suo%馬長路%徐珂佳%劉羿杉%李惠翔
려신전%Zhenhe Suo%마장로%서가가%류예삼%리혜상
乳腺肿瘤%免疫表型分型%抗原,CD44%抗原,CD24%肿瘤细胞,培养的
乳腺腫瘤%免疫錶型分型%抗原,CD44%抗原,CD24%腫瘤細胞,培養的
유선종류%면역표형분형%항원,CD44%항원,CD24%종류세포,배양적
Breast neoplasms%Immunophenotyping%Antigens,CD44%Antigens,CD24%Tumor cells,cultured
目的 检测CD44+/CIY24-细胞在乳腺癌组织及细胞系中的分布及数量,探讨其与乳腺癌常用标志物表达和乳腺癌分子亚型的关系.方法 采用免疫组织化学SP双染及单染法,分别检测了60例乳腺浸润性导管癌中的CD44及C1724的共表达情况和ER、PR、HER2、人雌激素诱导蛋白PS2、bcl-2、nm23的单独表达情况,同时检测了三种乳腺痛细胞系(MCF-7、MDA-MB-468及MDA-MB-231)中CD44及CD24的表达情况.结果 不同病例标本中CD44+/C1724-细胞的数量差异较大,分布无明显规律,总阳性率为65.0%;CD44+/CD24-细胞数量与患者年龄、肿瘤大小、淋巴结转移情况及ER、PR、HER-2、人雌激素诱导蛋白PS2、bcl-2、nm23表达情况无关(P均>0.05);CD44+/CD24-细胞数量与乳腺癌分子亚型无关.CD44+/CIY24-细胞在MCF-7、MDA-MB-468及MDA-MB-231细胞系中的比例分别为<1%、5%及>80%.结论 CD44+/CD24-细胞存在于部分乳腺癌组织及细胞系中,其数量及分布与乳腺癌的分子亚型和临床病理参数无直接关系.
目的 檢測CD44+/CIY24-細胞在乳腺癌組織及細胞繫中的分佈及數量,探討其與乳腺癌常用標誌物錶達和乳腺癌分子亞型的關繫.方法 採用免疫組織化學SP雙染及單染法,分彆檢測瞭60例乳腺浸潤性導管癌中的CD44及C1724的共錶達情況和ER、PR、HER2、人雌激素誘導蛋白PS2、bcl-2、nm23的單獨錶達情況,同時檢測瞭三種乳腺痛細胞繫(MCF-7、MDA-MB-468及MDA-MB-231)中CD44及CD24的錶達情況.結果 不同病例標本中CD44+/C1724-細胞的數量差異較大,分佈無明顯規律,總暘性率為65.0%;CD44+/CD24-細胞數量與患者年齡、腫瘤大小、淋巴結轉移情況及ER、PR、HER-2、人雌激素誘導蛋白PS2、bcl-2、nm23錶達情況無關(P均>0.05);CD44+/CD24-細胞數量與乳腺癌分子亞型無關.CD44+/CIY24-細胞在MCF-7、MDA-MB-468及MDA-MB-231細胞繫中的比例分彆為<1%、5%及>80%.結論 CD44+/CD24-細胞存在于部分乳腺癌組織及細胞繫中,其數量及分佈與乳腺癌的分子亞型和臨床病理參數無直接關繫.
목적 검측CD44+/CIY24-세포재유선암조직급세포계중적분포급수량,탐토기여유선암상용표지물표체화유선암분자아형적관계.방법 채용면역조직화학SP쌍염급단염법,분별검측료60례유선침윤성도관암중적CD44급C1724적공표체정황화ER、PR、HER2、인자격소유도단백PS2、bcl-2、nm23적단독표체정황,동시검측료삼충유선통세포계(MCF-7、MDA-MB-468급MDA-MB-231)중CD44급CD24적표체정황.결과 불동병례표본중CD44+/C1724-세포적수량차이교대,분포무명현규률,총양성솔위65.0%;CD44+/CD24-세포수량여환자년령、종류대소、림파결전이정황급ER、PR、HER-2、인자격소유도단백PS2、bcl-2、nm23표체정황무관(P균>0.05);CD44+/CD24-세포수량여유선암분자아형무관.CD44+/CIY24-세포재MCF-7、MDA-MB-468급MDA-MB-231세포계중적비례분별위<1%、5%급>80%.결론 CD44+/CD24-세포존재우부분유선암조직급세포계중,기수량급분포여유선암적분자아형화림상병리삼수무직접관계.
Objective To study the distribution and quantity of CD44VCD24- cells in breast cancer tissue and the cell lines,and as well as its correlation with the expression of various breast cancer markers and molecular subtyping of breast carcinoma.Methods The expression of CD44 / CD24,estrogen receptor,progesterone receptor,HER2,human estrogen-induced protein PS2,bcl-2 and nm23 in 60 cases of invasive ductal carcinoma of breast were studied by either single or double immunohistochemical staining.The co-expression of CD44 and CD24 in 3 breast cancer cell lines (MCF-7,MDA-MB-468,and MDA-MB- 231) was also examined.Results The quantity and distribution of CD44 + /CD24- cells varied greatly and no specific patterns were identified.The percentage of CD44 + /CD24- in breast cancer was 65%.The amount of CD44+/CD24- cells did not correlate with the age of patients,lymph node metastasis,tumor size,molecular subtypes and expression of various breast cancer markers in breast carcinoma.The proportion of CD44+/CD24- cells in MCF-7,MDA-MB-468,and MDA-MB-231 cell lines was < 1%,5% and > 80% ,respectively.Conclusions CD44+ /CD24- cells are demonstrated in certain breast cancer tissues and cell lines.However,there is no relationship obtained between the quantity or the distribution of these cells and the molecular subtyping or the clinicopathologic parameters in breast cancer.
