CAJ | 학술논문

目的研究垂体瘤转化基因在实验大鼠垂体腺瘤组织中的表达规律,探讨垂体瘤转化基因在垂体腺瘤形成过程中的作用.方法实验用雌性Wistar大鼠40只,摘除双侧卵巢,将大鼠完全随机分为实验组和对照组.通过腹腔注射苯甲酸雌二醇建立大鼠垂体腺瘤模型,分别对实验组和对照组大鼠垂体组织中垂体瘤转化基因进行定性和半定量分析,应用SPSS 11.5统计软件对资料处理分析.结果实验组大鼠垂体质量为(52.35 ±4.97)mg,大约为对照组垂体质量(12.02±0.77)mg的4倍,差异有统计学意义(t=29.98,P=0.000).实验组大鼠血清泌乳素浓度[(198.23±93.07)ng/L]大约是对照组血清泌乳素浓度[(50.12 ±14.24)ng/L]的4倍,差异有统计学意义(t=7.035,P=0.000).对垂体瘤转化基因表达进行半定量分析,结果对照组为(0.24±0.08),实验组为(1.41±0.13).结论雌激素诱导大鼠可以形成垂体泌乳素腺瘤;垂体瘤转化基因参与调节雌激素诱导的大鼠垂体腺瘤形成.
목적 연구수체류전화기인재실험대서수체선류조직중적표체규률,탐토수체류전화기인재수체선류형성과정중적작용.방법 실험용자성Wistar대서40지,적제쌍측란소,장대서완전수궤분위실험조화대조조.통과복강주사분갑산자이순건립대서수체선류모형,분별대실험조화대조조대서수체조직중수체류전화기인진행정성화반정량분석,응용SPSS 11.5통계연건대자료처리분석.결과 실험조대서수체질량위(52.35 ±4.97)mg,대약위대조조수체질량(12.02±0.77)mg적4배,차이유통계학의의(t=29.98,P=0.000).실험조대서혈청비유소농도[(198.23±93.07)ng/L]대약시대조조혈청비유소농도[(50.12 ±14.24)ng/L]적4배,차이유통계학의의(t=7.035,P=0.000).대수체류전화기인표체진행반정량분석,결과대조조위(0.24±0.08),실험조위(1.41±0.13).결론 자격소유도대서가이형성수체비유소선류;수체류전화기인삼여조절자격소유도적대서수체선류형성.
Objective The aim of this study was to explore expression of pituitary tumor transforming gene (PTTG) in estrogen-induced pituitary tumorigenesis in rats, to investigate its role in this process. Methods Forty female Wistar rats of 8 weeks old were used in our study. All animals were surgically ovariectomized before experi-ment. The female Wistar rats were induced with benzoic acid estradiol by peritoneal injection. Expression of PTTG mRNA levels in the pituitary glands were detected by semi-quantitative reverse transcription polymerase chain reac-tion ( RT-PCR). The data for PTTG mRNA levels were analyzed by the soft ware of SPSS 11.5 for windows.Results Significant increases in pituitary weight were noted three months after peritoneal injection of estradiol. The serum prolactin concentration also increased sharply in the rats of estrogenized groups. The histopathological exami-nation for pituitary tissues in experimental groups was also consistent with the diagnosis of pituitary adenoma. The expression level of PTTG mRNA in pituitary tumors was significantly higher than that of control groups. Conclusion It can be inferred from our study that chronic treatment with estradiol can result in pituitary adenoma in female Wist-ar rats. PTTG mRNA in the pituitary glands is regulated by estradiol with possible involvement in estradiol induced tumorigenesis in rats.