中华糖尿病杂志
中華糖尿病雜誌
중화당뇨병잡지
CHINES JOURNAL OF DLABETES MELLITUS
2012年
8期
479-482
,共4页
王大光%杨晓枫%马立清%靳迪%李传宝%张传宝%周绮%郭健
王大光%楊曉楓%馬立清%靳迪%李傳寶%張傳寶%週綺%郭健
왕대광%양효풍%마립청%근적%리전보%장전보%주기%곽건
糖尿病%血红蛋白A,糖基化%临床实验室技术
糖尿病%血紅蛋白A,糖基化%臨床實驗室技術
당뇨병%혈홍단백A,당기화%림상실험실기술
Diabetes mellitus%Hemoglobin A,glycated%Clinical laboratory techniques
目的 研究不同方法检测静脉血与末梢血糖化血红蛋白(HbA1c)的差异性.方法 选取2011年1月到10月卫生部北京医院门诊糖尿病患者91例,其中男38例,女53例,空腹采集静脉血置入4管2 ml的乙二胺四乙酸二钾抗凝管中,同时采集手指血32μl、耳血32μl肝素抗凝备用.血样立即分别送解放军总医院、卫生部北京医院、卫生部临床检验中心采用G7检测及社区门诊部用DCA2000+检测样本HbA1c水平.分析比较不同年龄组间HbA1c的水平差异、DCA2000+检测静脉血、手指血和耳血HbA1c的水平差异、G7检测静脉血及手指预稀释血HbA1c的水平差异以及不同检测单位以及检测方法间其HbA1c水平的差异.两组间比较采用Mann-Whitney检验,多组间比较采用Kruskal-Wallis检验.结果 DCA2000+ HbA1c分析仪的批内、批间差异均＜4％;3家医院的G7HbAl c分析仪批内、批间差异均＜2％,均符合美国糖尿病学会(ADA)的HbA1c分析仪差异＜5％的质量标准.卫生部临床检验中心采用G7法检测不同年龄组间其HbA1c水平差异无统计学意义(x2=3.969,P＞0.05).社区门诊部采用DCA2000+检测静脉血、手指血、耳血的HbA1c水平差异亦无统计学意义(x2=0.474,P＞0.05);北京医院采用G7检测静脉血、预稀释手指血HbA1c间差异无统计学意义(Z=-0.092,P＞0.05).四家医疗机构采用不同检测方法及不同标本间检测的HbA1c水平间差异无统计学意义(x2 =5.513,P＞0.05).结论 四家医疗机构的两款HbA1c检测仪器一致性和重复性好,结果呈正相关,可以互认;静脉血和末梢血检测HbA1c结果无差异.
目的 研究不同方法檢測靜脈血與末梢血糖化血紅蛋白(HbA1c)的差異性.方法 選取2011年1月到10月衛生部北京醫院門診糖尿病患者91例,其中男38例,女53例,空腹採集靜脈血置入4管2 ml的乙二胺四乙痠二鉀抗凝管中,同時採集手指血32μl、耳血32μl肝素抗凝備用.血樣立即分彆送解放軍總醫院、衛生部北京醫院、衛生部臨床檢驗中心採用G7檢測及社區門診部用DCA2000+檢測樣本HbA1c水平.分析比較不同年齡組間HbA1c的水平差異、DCA2000+檢測靜脈血、手指血和耳血HbA1c的水平差異、G7檢測靜脈血及手指預稀釋血HbA1c的水平差異以及不同檢測單位以及檢測方法間其HbA1c水平的差異.兩組間比較採用Mann-Whitney檢驗,多組間比較採用Kruskal-Wallis檢驗.結果 DCA2000+ HbA1c分析儀的批內、批間差異均＜4％;3傢醫院的G7HbAl c分析儀批內、批間差異均＜2％,均符閤美國糖尿病學會(ADA)的HbA1c分析儀差異＜5％的質量標準.衛生部臨床檢驗中心採用G7法檢測不同年齡組間其HbA1c水平差異無統計學意義(x2=3.969,P＞0.05).社區門診部採用DCA2000+檢測靜脈血、手指血、耳血的HbA1c水平差異亦無統計學意義(x2=0.474,P＞0.05);北京醫院採用G7檢測靜脈血、預稀釋手指血HbA1c間差異無統計學意義(Z=-0.092,P＞0.05).四傢醫療機構採用不同檢測方法及不同標本間檢測的HbA1c水平間差異無統計學意義(x2 =5.513,P＞0.05).結論 四傢醫療機構的兩款HbA1c檢測儀器一緻性和重複性好,結果呈正相關,可以互認;靜脈血和末梢血檢測HbA1c結果無差異.
목적 연구불동방법검측정맥혈여말소혈당화혈홍단백(HbA1c)적차이성.방법 선취2011년1월도10월위생부북경의원문진당뇨병환자91례,기중남38례,녀53례,공복채집정맥혈치입4관2 ml적을이알사을산이갑항응관중,동시채집수지혈32μl、이혈32μl간소항응비용.혈양립즉분별송해방군총의원、위생부북경의원、위생부림상검험중심채용G7검측급사구문진부용DCA2000+검측양본HbA1c수평.분석비교불동년령조간HbA1c적수평차이、DCA2000+검측정맥혈、수지혈화이혈HbA1c적수평차이、G7검측정맥혈급수지예희석혈HbA1c적수평차이이급불동검측단위이급검측방법간기HbA1c수평적차이.량조간비교채용Mann-Whitney검험,다조간비교채용Kruskal-Wallis검험.결과 DCA2000+ HbA1c분석의적비내、비간차이균＜4％;3가의원적G7HbAl c분석의비내、비간차이균＜2％,균부합미국당뇨병학회(ADA)적HbA1c분석의차이＜5％적질량표준.위생부림상검험중심채용G7법검측불동년령조간기HbA1c수평차이무통계학의의(x2=3.969,P＞0.05).사구문진부채용DCA2000+검측정맥혈、수지혈、이혈적HbA1c수평차이역무통계학의의(x2=0.474,P＞0.05);북경의원채용G7검측정맥혈、예희석수지혈HbA1c간차이무통계학의의(Z=-0.092,P＞0.05).사가의료궤구채용불동검측방법급불동표본간검측적HbA1c수평간차이무통계학의의(x2 =5.513,P＞0.05).결론 사가의료궤구적량관HbA1c검측의기일치성화중복성호,결과정정상관,가이호인;정맥혈화말소혈검측HbA1c결과무차이.
Objective To compare the differences of different methods for glycated hemoglobin ( HbAl c) measurement with venous and capillary blood samples.Methods Total of 91 diabetes outpatients (38 males and 53 females) were selected in Beijing Hospital from January to October 2011.Four fasting venous blood samples were collected in 2 ml EDTA-K2 anti-coagulation vacuum tubes,meanwhile,32 μl heparin anti-coagulated capillary blood samples were obtained from the ear lobe and the fingertip.These blood samples were immediately measured by G7 in PLA General Hospital,Beijing Hospital and National Center for Clinical Laboratories,or measured by DCA2000 + in community clinics.The blood HbA1c levels were compared among different age groups,different samples on DCA2000 + (from venous,ear lobes,fingertip) or on G7 (from venous and fingertip) and different laboratories and methods.Mann-Whitney test was applied in data comparison between to groups and Kruskal-Wallis test among muhiple groups.Results The intra-and inter-assay coefficients of variation (CV) of the DCA2000 + analyzer were all less than 4％,and the intra- and inter-assay CV of the G7 analyzer from 3 hospitals were all ＜ 2％,the results were all accorded with the American Diabetes Association requirement of a CV ＜ 5％ for HbA1c analyzers. No significant statistical differences in HbA1c level was observed among different age groups with the G7measurement in National Center for Clinical Laboratories( x2 =3.969,P ＞ 0.05 ).There was no significant differences in HbA1c levels measured by DCA2000 + with venous,fingertip and ear lobe blood samples in community clinics ( x2 =0.474,P ＞ 0.05 ).The results of G7 analysis in Beijing Hospital did not differ significantly with venous blood sample and prediluted fingertip blood( Z =-0.092,P ＞ 0.05 ).There was no statistical differences in HbA1c levels measured in 4 medical institutions using different methods( x2 =5.513,P ＞0.05).Conclusions Two detecting methods of HbA1c used in the 4 medical facilities have good coherency and repeatability,the results are correlated and can be mutually accredited. The HbA1c levels in venous and capillary blood samples are the same.
