中华肾脏病杂志
中華腎髒病雜誌
중화신장병잡지
2011年
4期
288-292
,共5页
郑云%郝丽%潘梦舒%丁楠
鄭雲%郝麗%潘夢舒%丁楠
정운%학려%반몽서%정남
糖尿病肾病%足细胞%雷公藤多苷
糖尿病腎病%足細胞%雷公籐多苷
당뇨병신병%족세포%뢰공등다감
Diabetic nephropathies%Podocytes%Triperygium wilfordii multiglucoside
目的 探讨雷公藤多苷(TWP)对糖尿病肾病大鼠足细胞病变的影响.方法 SD大鼠100只,按随机数字表法分为正常对照组(A组)、糖尿病组(B组)与TWP治疗组(C 组).并将C组按4、8、16 mg·kg-1·d-1不同给药剂量分为3组(Ca、Cb、Cc).糖尿病组与TWP 治疗组大鼠分别给予链脲菌素(STZ)一次性腹腔注射建立糖尿病大鼠模型.12周后检测24 h 尿蛋白量、血肌酐(Scr)、尿素氮(BUN)、外周血白细胞(WBC)、血糖(Glu)、肾质量/体质量(KW/BW)的变化;HE染色检测肾组织病理变化;透射电镜测量足细胞超微结构变化;免疫荧光法检测肾皮质nephrin和podocin蛋白表达.结果 (1)与A组比较,B组及C组Scr、BUN 增高(P＜0.05);Glu、KW/BW和24 h尿蛋白量显著增高(P＜0.01),除Cc组(3/20)出现肝酶(ALT、AST)增高及WBC下降外,各组ALT、AST、WBC差异无统计学意义;肾且庠皮质nephrin 和podocin蛋白表达减少,肾小球、小管间质及足细胞病变明显.(2)与B组比较,C组KW/BW 和24 h尿蛋白量降低(P＜0.01),肾脏皮质nephrin和podocin蛋白表达增高(均P＜0.01),肾小球、小管间质及足细胞病变明显减轻,并随雷公藤多苷剂量的增加而越加明显.结论 TWP对糖尿病大鼠足细胞病变具有保护作用,并与剂量相关.其部分机制可能与上调nephrin 和podocin蛋白表达有关.
目的 探討雷公籐多苷(TWP)對糖尿病腎病大鼠足細胞病變的影響.方法 SD大鼠100隻,按隨機數字錶法分為正常對照組(A組)、糖尿病組(B組)與TWP治療組(C 組).併將C組按4、8、16 mg·kg-1·d-1不同給藥劑量分為3組(Ca、Cb、Cc).糖尿病組與TWP 治療組大鼠分彆給予鏈脲菌素(STZ)一次性腹腔註射建立糖尿病大鼠模型.12週後檢測24 h 尿蛋白量、血肌酐(Scr)、尿素氮(BUN)、外週血白細胞(WBC)、血糖(Glu)、腎質量/體質量(KW/BW)的變化;HE染色檢測腎組織病理變化;透射電鏡測量足細胞超微結構變化;免疫熒光法檢測腎皮質nephrin和podocin蛋白錶達.結果 (1)與A組比較,B組及C組Scr、BUN 增高(P＜0.05);Glu、KW/BW和24 h尿蛋白量顯著增高(P＜0.01),除Cc組(3/20)齣現肝酶(ALT、AST)增高及WBC下降外,各組ALT、AST、WBC差異無統計學意義;腎且庠皮質nephrin 和podocin蛋白錶達減少,腎小毬、小管間質及足細胞病變明顯.(2)與B組比較,C組KW/BW 和24 h尿蛋白量降低(P＜0.01),腎髒皮質nephrin和podocin蛋白錶達增高(均P＜0.01),腎小毬、小管間質及足細胞病變明顯減輕,併隨雷公籐多苷劑量的增加而越加明顯.結論 TWP對糖尿病大鼠足細胞病變具有保護作用,併與劑量相關.其部分機製可能與上調nephrin 和podocin蛋白錶達有關.
목적 탐토뢰공등다감(TWP)대당뇨병신병대서족세포병변적영향.방법 SD대서100지,안수궤수자표법분위정상대조조(A조)、당뇨병조(B조)여TWP치료조(C 조).병장C조안4、8、16 mg·kg-1·d-1불동급약제량분위3조(Ca、Cb、Cc).당뇨병조여TWP 치료조대서분별급여련뇨균소(STZ)일차성복강주사건립당뇨병대서모형.12주후검측24 h 뇨단백량、혈기항(Scr)、뇨소담(BUN)、외주혈백세포(WBC)、혈당(Glu)、신질량/체질량(KW/BW)적변화;HE염색검측신조직병리변화;투사전경측량족세포초미결구변화;면역형광법검측신피질nephrin화podocin단백표체.결과 (1)여A조비교,B조급C조Scr、BUN 증고(P＜0.05);Glu、KW/BW화24 h뇨단백량현저증고(P＜0.01),제Cc조(3/20)출현간매(ALT、AST)증고급WBC하강외,각조ALT、AST、WBC차이무통계학의의;신차상피질nephrin 화podocin단백표체감소,신소구、소관간질급족세포병변명현.(2)여B조비교,C조KW/BW 화24 h뇨단백량강저(P＜0.01),신장피질nephrin화podocin단백표체증고(균P＜0.01),신소구、소관간질급족세포병변명현감경,병수뢰공등다감제량적증가이월가명현.결론 TWP대당뇨병대서족세포병변구유보호작용,병여제량상관.기부분궤제가능여상조nephrin 화podocin단백표체유관.
Objective To explore the effect of triperygium wilfordii polyglucoside(TWP) on the podocytes of rats with diabetes nephropathy(DN). Methods One hundred SD rats were randomly divided into normal control group (Group A),DN group (Group B),TWP group(Group C).TWP group was divided into 3 subgroups(Ca,Cb,Cc)according to the different doses 4,8,16mg·kg-1·d-1,respectively.Rats in DN group and TWp group were given streptozocin(STZ)by intraperitoneal injection to establish animal model of diabetes.After 12 weeks,24 h urinary protein excretion rate(UAER),BUN,Scr,white blood cell(WBC),blood glucose(Glu),and kidney weight (KW)/body weight(BW)were determined.The renal pathological changes were evaluated by HE staining.The structural change of podocytes was observed by transmission electron microscope.The expressions of nephrin and podocin were evaluated by immunofluorescence staining. Results (1)Compared to group A,Scr,BUN,Glu,KW/BW,and UAER were significantly higher(P＜0.05)in group B and group C.Whereas the elevated liver enzymes and the decreased WBC were presented in group Cc (3/20). Compared to group A, the protein expressions of nephrin and podocin in nephridial tissue were lower, and the significant differences of pathomorphology in glomerulus,tubules and podocytes were observed in group B and group C. (2) Compared to group B, KW/BW and UAER were lower in group C (P＜0.01); the protein expressions of nephrin and podocin were higher in nephridial tissue; the pathomorphological improvements were exhibited in glomerulus,tubules and podocytes in group C, paralleled with the increase of TWP dose (P＜0.05 or P＜0.01).Conclusions TWP may exert the protective effect on podocytes in diabetic nephropathy rats,dependent on the dose of TWP. The mechanism may be associated with the up-regulatied expressions of nephrin and podocin.
