CAJ | 학술논문

目的探讨基因芯片技术在心血管外科临床及科研中的应用价值,应用表达谱基因芯片筛选体外循环(CPB)前后心肌组织的差异表达基因,为CPB炎性反应的研究提供线索.方法取CPB开始及CPB结束即刻的右房组织,用BD AtlasTM cDNA Expression Arrays表达谱基因芯片对比细胞因子的差异表达,应用RT-PCR对结果进行验证.结果将基因芯片技术成功应用于CPB研究,并得到CPB前后心肌细胞因子基因表达谱.CPB后心肌白细胞介素-6(IL-6)、γ-干扰素(IFN-γ)、Wnt5a、肿瘤坏死因子受体超家族成员1B(TNFRSF1B)、胎盘生长因子(PIGF)和MFNG mRNA表达增强.结论基因芯片技术在CPB后细胞因子变化的研究中有一定应用价值.表达谱基因芯片初步筛选出了CPB后的差异表达基因,这些基因可能参与了CPB引起的炎性反应及其他病理生理反应.
목적 탐토기인심편기술재심혈관외과림상급과연중적응용개치,응용표체보기인심편사선체외순배(CPB)전후심기조직적차이표체기인,위CPB염성반응적연구제공선색.방법 취CPB개시급CPB결속즉각적우방조직,용BD AtlasTM cDNA Expression Arrays표체보기인심편대비세포인자적차이표체,응용RT-PCR대결과진행험증.결과 장기인심편기술성공응용우CPB연구,병득도CPB전후심기세포인자기인표체보.CPB후심기백세포개소-6(IL-6)、γ-간우소(IFN-γ)、Wnt5a、종류배사인자수체초가족성원1B(TNFRSF1B)、태반생장인자(PIGF)화MFNG mRNA표체증강.결론 기인심편기술재CPB후세포인자변화적연구중유일정응용개치.표체보기인심편초보사선출료CPB후적차이표체기인,저사기인가능삼여료CPB인기적염성반응급기타병리생리반응.
Objestive Systemic inflarmmation may be triggered by injury, hypothermia, ischemia-reperfusion and the contact of the blood with foreign body during cardiopulmonary bypass (CPB). To determine the application values of gene chip technique in the clinical practice and the study of cardiovascular stagery, as well as to provide clues to the study of inflammatory responess during CPB, microarry for gene expression profiles was used to identify the differences in the gene expression of myocardium between pre-and post- CPB. Methods Six adult patients who underwent CPB from March to May in 2003 were involved. Samples of right atrium were col- lected before and at immediate end of CPB. BD AtlasTM cDNA Expression Arrays was used to identify the differences in the gene ex- pression of cytokines. The results were compared with that of semi-quantative RT-PCR. Resellts The mean age of 6 patients (5 males and 1 female) was (32.67± 11.72) years. The baseline heart function was gradeⅡin 3 cases and grade Ⅲ in 3 other cases. The baseline left ventricular ejection fraction(LVEF)was (58.17±7.91)%. The mere duration was (91.67±43.88) minutes for CPB and was (58.67±43.46) minutes for aorta blocking. The minimum nasopharynx/rectal temperture was (29.37±1.90)℃/ (32.15±1.52)℃. Gene expression profiles of cytokines in the myocardium pre- and post-CPB were analysed successfully. The ex- pression of IL-6, IFN-γ,Wnt5a, TNFRSF1B, a member of tumor necrosis factor receptor superfamily, PIGF and MFNG in the myo- cardium were unpregulated after CPB. Conclusion Microarray technique is applicable in the study of cytokines changes dying CPB. cDNA microarray identified pleliminarily the differences in the gene expression between pre- and post-CPB. These genes may be in- valved in inflammation and other psthophysiological responses incuced by CPB. The myocardiym is probably one of the major sources of cytokines during CPB. Further study may be helpful in understanding the llngthe development of inflammation during CPB, and eventually, reducing the post-operative complications.