中华显微外科杂志
中華顯微外科雜誌
중화현미외과잡지
Chinese Journal of Microsurgery
2009年
1期
43-46,封3
,共5页
T细胞疫苗%FK506%异种移植%外周神经
T細胞疫苗%FK506%異種移植%外週神經
T세포역묘%FK506%이충이식%외주신경
T cell vaccination%FK506%Heterogenic transplantation%Peripheral nerve
目的 研究T细胞疫苗接种联合应用FK506对大鼠异种神经移植排斥反应的抑制作用. 方法 利用BABL/c小鼠的脾细胞免疫SD大鼠,取后者脾细胞活化所制备的T细胞疫苗对异种神经移植受体的SD大鼠进行免疫,移植后联合应用FK506.以异种神经移植后混合淋巴细胞培养、微量细胞毒测定、移植神经的组织学观察和胫前肌湿重测定等,对该方法抗大鼠异种神经移植排斥反应的作用加以分析. 结果 和对照组相比较,T细胞疫苗接种联合FK506使神经移植大鼠胫前肌湿重明显增加(P＜0.05),淋巴细胞转化率等排斥反应指标差异也具有统计学意义(P＜0.05). 结论 T细胞疫苗接种联合应用FK506能显著抑制大鼠异种神经移植的免疫排斥反应.
目的 研究T細胞疫苗接種聯閤應用FK506對大鼠異種神經移植排斥反應的抑製作用. 方法 利用BABL/c小鼠的脾細胞免疫SD大鼠,取後者脾細胞活化所製備的T細胞疫苗對異種神經移植受體的SD大鼠進行免疫,移植後聯閤應用FK506.以異種神經移植後混閤淋巴細胞培養、微量細胞毒測定、移植神經的組織學觀察和脛前肌濕重測定等,對該方法抗大鼠異種神經移植排斥反應的作用加以分析. 結果 和對照組相比較,T細胞疫苗接種聯閤FK506使神經移植大鼠脛前肌濕重明顯增加(P＜0.05),淋巴細胞轉化率等排斥反應指標差異也具有統計學意義(P＜0.05). 結論 T細胞疫苗接種聯閤應用FK506能顯著抑製大鼠異種神經移植的免疫排斥反應.
목적 연구T세포역묘접충연합응용FK506대대서이충신경이식배척반응적억제작용. 방법 이용BABL/c소서적비세포면역SD대서,취후자비세포활화소제비적T세포역묘대이충신경이식수체적SD대서진행면역,이식후연합응용FK506.이이충신경이식후혼합림파세포배양、미량세포독측정、이식신경적조직학관찰화경전기습중측정등,대해방법항대서이충신경이식배척반응적작용가이분석. 결과 화대조조상비교,T세포역묘접충연합FK506사신경이식대서경전기습중명현증가(P＜0.05),림파세포전화솔등배척반응지표차이야구유통계학의의(P＜0.05). 결론 T세포역묘접충연합응용FK506능현저억제대서이충신경이식적면역배척반응.
Objective To investigate the anti-rejection effect of T cell vaccination combined with FK506 of heterogenic transplantation on rat nerve. Methods SD rats were vaccinated by the spleen cells of BABL/c mice; T cell vaccines were activated and vaccinated in the spleen cells of these SD rats. The SD rats of nerve transplantation recipient were vaccinated with T cells combined with FKS06. The mixture lymphocytes culture, microcytotoxicity assay, histological alteration of nerves, humid weights of tibial anterior muscle were observed to confirm the anti-rejection effect of T cell vaccination combined with FK506 treatment. Results The humid weights of tibial anterior muscle of the vaccinated rats combined with FKS06 treatment were statistically different from those in control group (P ＜ 0.05), and so as the index of rejection (e.g. lymphocytes transformation efficiency) (P ＜ 0.05). Conclusion T cell vaccination combined with FK506 treatment can inhibit the rejection of heterogenic transplantation on rat peripheral nerve.
