CAJ | 학술논문

目的观察电针对豚鼠庆大霉素(gentamicin,GE)耳中毒引起的耳蜗琥珀酸脱氢酶(succinate de-hydrogenase,SDH)变化的影响.方法将动物随机分成3组:GE组动物肌肉注射GE80 mg@kg-1.d-1,连续注射20天:针刺组肌肉注射GE同GE组,每天电针双侧听宫、翳风、肾俞穴1次,持续刺激15 min;对照组动物不做任何处理.以脑干听觉诱发电位(BAEP)、SDH组织化学检测为观察指标.结果对照组BAEP和SDH两项指标无明显变化;GE组BAEP反应阈明显升高,SDH显色变淡或消失,毛细胞受损显著;针刺组BAEP反应阈升高幅度和SDH变化及毛细胞损害程度均明显低于GE组.结论针刺组能减轻GE耳毒性,对SDH有保护作用.
목적관찰전침대돈서경대매소(gentamicin,GE)이중독인기적이와호박산탈경매(succinate de-hydrogenase,SDH)변화적영향.방법장동물수궤분성3조:GE조동물기육주사GE80 mg@kg-1.d-1,련속주사20천:침자조기육주사GE동GE조,매천전침쌍측은궁、예풍、신유혈1차,지속자격15 min;대조조동물불주임하처리.이뇌간은각유발전위(BAEP)、SDH조직화학검측위관찰지표.결과대조조BAEP화SDH량항지표무명현변화;GE조BAEP반응역명현승고,SDH현색변담혹소실,모세포수손현저;침자조BAEP반응역승고폭도화SDH변화급모세포손해정도균명현저우GE조.결론침자조능감경GE이독성,대SDH유보호작용.
Objective: To observe the effect of dectroacupuncture (EA) on succinate dehydrogenase (SDH) ofgentamycin (GE) induced ototoxic cochlear hair cells. Methods: Preyer's reflex normal guinea pigs were seldectedand randomized into three groups: GE group, EA group and control group. Brainstem auditory evoked potential(BAEP) and SDH in the cochlear hair cells were taken as indexes. In the animals of GE group GE was alone inject-ed intramuscularly 80 mg kg- 1. d- 1 for successive 20 days, while in the animals of EA group GE and additional EAwere applied once a day for 15 minutes each time on Tinggong (SI 19), Yifeng(SJ17) and Shenshu (LJB23)points.Results: In GE group BAEP reaction threshold rose markedly, while in EA group it rose slightly. The differencewas significant between the two groups ( P＜ 0.05 ). The change of SDH within cochlear hair cells and the degreeof hair cell lesion in the EA group were lower than those in GE group. Conclusions: EA therapy could relieve GEototoxicity, maintain SDH in cochlear hair cells, which might be a possible mechanism of action of EA.