CAJ | 학술논문

目的研究Ⅰ型胶原对人胚骨膜来源成骨细胞生物学特性的影响，为Ⅰ型胶原在组织工程人工骨中的应用提供依据。方法 [HTSS在不同浓度Ⅰ型胶原涂层上培养成骨细胞，用细胞计数法研究成骨细胞粘附情况，3H-TdR掺入实验观察成骨细胞增殖能力，通过检测成骨细胞胶原、骨钙素和碱性磷酸酶的合成情况，以不涂层Ⅰ型胶原为对照组，比较骨膜来源成骨细胞成骨能力的改变。结果 Ⅰ型胶原对成骨细胞发挥以下作用：①增加粘附细胞数量，且在25μg/ml浓度达最大效应；②减弱成骨细胞增殖能力，且以12.5μg/ml以上浓度作用显著（P<0.05）;③轻度减弱成骨细胞Ⅰ型胶原合成能力，以25μg/ml以上浓度作用显著（P<0.05）；④增加骨钙素合成，以6.25μg/ml以上浓度作用显著（P<0.05），25μg/ml浓度达最大效应；⑤增加成骨细胞碱性磷酸酶活性，以12.5μg/ml以上浓度作用显著（P<0.05）。结论 Ⅰ型胶原能促进成骨细胞的粘附与分化；支架材料上复合Ⅰ型胶原涂层可增强成骨细胞的成骨能力；Ⅰ型胶原最佳复合浓度为25μg/ml。
목적 연구Ⅰ형효원대인배골막래원성골세포생물학특성적영향，위Ⅰ형효원재조직공정인공골중적응용제공의거。방법 [HTSS재불동농도Ⅰ형효원도층상배양성골세포，용세포계수법연구성골세포점부정황，3H-TdR참입실험관찰성골세포증식능력，통과검측성골세포효원、골개소화감성린산매적합성정황，이불도층Ⅰ형효원위대조조，비교골막래원성골세포성골능력적개변。결과 Ⅰ형효원대성골세포발휘이하작용：①증가점부세포수량，차재25μg/ml농도체최대효응；②감약성골세포증식능력，차이12.5μg/ml이상농도작용현저（P<0.05）;③경도감약성골세포Ⅰ형효원합성능력，이25μg/ml이상농도작용현저（P<0.05）；④증가골개소합성，이6.25μg/ml이상농도작용현저（P<0.05），25μg/ml농도체최대효응；⑤증가성골세포감성린산매활성，이12.5μg/ml이상농도작용현저（P<0.05）。결론 Ⅰ형효원능촉진성골세포적점부여분화；지가재료상복합Ⅰ형효원도층가증강성골세포적성골능력；Ⅰ형효원최가복합농도위25μg/ml。
Objective To study the influence of type Ⅰ coll agen(COL Ⅰ) onthe cell behavior of human periosteous osteoblasts(OB) and the application of type Ⅰ collagen in constructing bioactive artifical bone.Methods OB were cultured on dishes coated with bovine type Ⅰ collage n in different final concentrations. The cell adhesion was examined by the meth o ds of cell count, the proliferation of OB was studied by 3H-TdR, and the ost eoblastic ability was assessed by the synthesis of collagen, osteocalcin and alk aline phosphatase (ALP). Results OB cultured on type Ⅰ collage n layer had the following characteristicis: ① The amounts of adhesive cells we re maximal top in 25 μg/ml final concentration; ② The proliferation of OB was decreased above 12.5 μg/ml final concentration (P<0.05); ③ The synthesis of type Ⅰ collagen was reduced slightly (above 25 μg/ml,P<0.05); ④ The secretion of osteocalcin was increased markedly (above 6.25 μg/ml,P<0.05, w hich reached maximally in 25μg/ml); ⑤ The ALP activity was also increased (ab ove 12.5μg/ml, P<0.05). Conclusion Type Ⅰ collagen promot es the expression of osteoblastic phenotype and cell adhesion. When the scaffold materials for bone tissue engineering are coated with type Ⅰ collagen, the ost eogenesis of OB is enhanced to accelerate the tranformation course from artifici al bone to biological bone, the best final concentration is 25μg/ml.