背景:雷公藤具有抗炎、免疫抑制等作用,目前已被用于治疗类风湿关节炎等多种自身免疫性疾病.其化学成分复杂,其中雷公藤内酯醇被公认为是雷公藤中重要的有效成分之一.目的:建立Ⅱ型胶原诱导的关节炎大鼠模型,观察雷公藤内酯醇对其外周血清和关节腔中白细胞介素6、白细胞介素10以及肿瘤坏死因子α含量的影响.设计:随机对照动物实验.单位:华中科技大学同济医学院附属同济医院中西医结合科.材料:实验于2004-11/2005-07在武汉同济医院完成.选取清洁级健康雄性Wistar大白鼠50只(购于华中科技大学同济医学院实验动物中心,动物质量合格证号:scxk(鄂)2004-2007).雷公藤内酯醇(购于福建省医学科学研究所,临时生产无批号),纯度为98.5%以上.方法:①50只大鼠随机数字表法选10只作为正常对照组,其余建立关节炎模型.沿造模大鼠背部及尾根部分5点皮内注射Ⅱ型胶原乳剂,0.05 mL/点,15 d后分两点皮内激发注射.正常对照组予以生理盐水同法注射.初次免疫30 d后参照关节炎指数评分标准对造模效果进行评估,共20只大鼠评分在6分以上成功造模而用于实验,随机数字表法分为关节炎模型组、雷公藤内酯醇组,10只/组.②将雷公藤内酯醇用体积分数为0.05的丙二醇配制成注射液,其中含雷公藤内酯醇100mg/L,雷公藤内酯醇组按0.04mL/100g后腿肌注给药,每3 d用药1次,共30 d.正常对照组给予等量生理盐水,关节炎模型组给予等量的体积分数为0.05的丙二醇液.③给药30 d后取材,采用酶联免疫吸附法检测各组大鼠血清和膝关节腔液中白细胞介素6,10以及肿瘤坏死因子α的含量.主要观察指标:雷公藤内酯醇对外周血和关节腔液中肿瘤坏死因子α、白细胞介素6,10含量的影响.结果:实验选取清洁级雄性Wistar大鼠50只,10只作为正常对照,剩余40只大鼠共20只造模成功而进入结果分析.①关节炎模型组外周血和关节腔内的肿瘤坏死因子α含量最高,经雷公藤内酯醇治疗后均明显下降[(35.09±8.82),(15.35±3.56)ng/L;(44.17±8.94),(22.54±4.76)ng/L;P均＜0.01],且与正常对照组基本相似(P＞0.05).②关节炎模型组外周血和关节腔内的白细胞介素6含量最高,经雷公藤内酯醇治疗后均明显下降[(76.58±6.81),(42.45±5.72)ng/L;(88.69±10.56),(48.67±5.97)ng/L;P均＜0.01],但未能达到正常对照组水平(P＜0.05).③关节炎模型组外周血和关节腔内的白细胞介素10含量最低,经雷公藤内酯醇治疗后均明显升高[(17.53±2.07),(21.23±2.91)ng/L;(10.59±2.96),(14.74±1.85)ng/L;P均＜0.01],且与正常对照组基本相似(P＞0.05).结论:雷公藤内酯醇可能通过对细胞因子含量的调节治疗关节炎.
揹景:雷公籐具有抗炎、免疫抑製等作用,目前已被用于治療類風濕關節炎等多種自身免疫性疾病.其化學成分複雜,其中雷公籐內酯醇被公認為是雷公籐中重要的有效成分之一.目的:建立Ⅱ型膠原誘導的關節炎大鼠模型,觀察雷公籐內酯醇對其外週血清和關節腔中白細胞介素6、白細胞介素10以及腫瘤壞死因子α含量的影響.設計:隨機對照動物實驗.單位:華中科技大學同濟醫學院附屬同濟醫院中西醫結閤科.材料:實驗于2004-11/2005-07在武漢同濟醫院完成.選取清潔級健康雄性Wistar大白鼠50隻(購于華中科技大學同濟醫學院實驗動物中心,動物質量閤格證號:scxk(鄂)2004-2007).雷公籐內酯醇(購于福建省醫學科學研究所,臨時生產無批號),純度為98.5%以上.方法:①50隻大鼠隨機數字錶法選10隻作為正常對照組,其餘建立關節炎模型.沿造模大鼠揹部及尾根部分5點皮內註射Ⅱ型膠原乳劑,0.05 mL/點,15 d後分兩點皮內激髮註射.正常對照組予以生理鹽水同法註射.初次免疫30 d後參照關節炎指數評分標準對造模效果進行評估,共20隻大鼠評分在6分以上成功造模而用于實驗,隨機數字錶法分為關節炎模型組、雷公籐內酯醇組,10隻/組.②將雷公籐內酯醇用體積分數為0.05的丙二醇配製成註射液,其中含雷公籐內酯醇100mg/L,雷公籐內酯醇組按0.04mL/100g後腿肌註給藥,每3 d用藥1次,共30 d.正常對照組給予等量生理鹽水,關節炎模型組給予等量的體積分數為0.05的丙二醇液.③給藥30 d後取材,採用酶聯免疫吸附法檢測各組大鼠血清和膝關節腔液中白細胞介素6,10以及腫瘤壞死因子α的含量.主要觀察指標:雷公籐內酯醇對外週血和關節腔液中腫瘤壞死因子α、白細胞介素6,10含量的影響.結果:實驗選取清潔級雄性Wistar大鼠50隻,10隻作為正常對照,剩餘40隻大鼠共20隻造模成功而進入結果分析.①關節炎模型組外週血和關節腔內的腫瘤壞死因子α含量最高,經雷公籐內酯醇治療後均明顯下降[(35.09±8.82),(15.35±3.56)ng/L;(44.17±8.94),(22.54±4.76)ng/L;P均＜0.01],且與正常對照組基本相似(P＞0.05).②關節炎模型組外週血和關節腔內的白細胞介素6含量最高,經雷公籐內酯醇治療後均明顯下降[(76.58±6.81),(42.45±5.72)ng/L;(88.69±10.56),(48.67±5.97)ng/L;P均＜0.01],但未能達到正常對照組水平(P＜0.05).③關節炎模型組外週血和關節腔內的白細胞介素10含量最低,經雷公籐內酯醇治療後均明顯升高[(17.53±2.07),(21.23±2.91)ng/L;(10.59±2.96),(14.74±1.85)ng/L;P均＜0.01],且與正常對照組基本相似(P＞0.05).結論:雷公籐內酯醇可能通過對細胞因子含量的調節治療關節炎.
배경:뢰공등구유항염、면역억제등작용,목전이피용우치료류풍습관절염등다충자신면역성질병.기화학성분복잡,기중뢰공등내지순피공인위시뢰공등중중요적유효성분지일.목적:건립Ⅱ형효원유도적관절염대서모형,관찰뢰공등내지순대기외주혈청화관절강중백세포개소6、백세포개소10이급종류배사인자α함량적영향.설계:수궤대조동물실험.단위:화중과기대학동제의학원부속동제의원중서의결합과.재료:실험우2004-11/2005-07재무한동제의원완성.선취청길급건강웅성Wistar대백서50지(구우화중과기대학동제의학원실험동물중심,동물질량합격증호:scxk(악)2004-2007).뢰공등내지순(구우복건성의학과학연구소,림시생산무비호),순도위98.5%이상.방법:①50지대서수궤수자표법선10지작위정상대조조,기여건립관절염모형.연조모대서배부급미근부분5점피내주사Ⅱ형효원유제,0.05 mL/점,15 d후분량점피내격발주사.정상대조조여이생리염수동법주사.초차면역30 d후삼조관절염지수평분표준대조모효과진행평고,공20지대서평분재6분이상성공조모이용우실험,수궤수자표법분위관절염모형조、뢰공등내지순조,10지/조.②장뢰공등내지순용체적분수위0.05적병이순배제성주사액,기중함뢰공등내지순100mg/L,뢰공등내지순조안0.04mL/100g후퇴기주급약,매3 d용약1차,공30 d.정상대조조급여등량생리염수,관절염모형조급여등량적체적분수위0.05적병이순액.③급약30 d후취재,채용매련면역흡부법검측각조대서혈청화슬관절강액중백세포개소6,10이급종류배사인자α적함량.주요관찰지표:뢰공등내지순대외주혈화관절강액중종류배사인자α、백세포개소6,10함량적영향.결과:실험선취청길급웅성Wistar대서50지,10지작위정상대조,잉여40지대서공20지조모성공이진입결과분석.①관절염모형조외주혈화관절강내적종류배사인자α함량최고,경뢰공등내지순치료후균명현하강[(35.09±8.82),(15.35±3.56)ng/L;(44.17±8.94),(22.54±4.76)ng/L;P균＜0.01],차여정상대조조기본상사(P＞0.05).②관절염모형조외주혈화관절강내적백세포개소6함량최고,경뢰공등내지순치료후균명현하강[(76.58±6.81),(42.45±5.72)ng/L;(88.69±10.56),(48.67±5.97)ng/L;P균＜0.01],단미능체도정상대조조수평(P＜0.05).③관절염모형조외주혈화관절강내적백세포개소10함량최저,경뢰공등내지순치료후균명현승고[(17.53±2.07),(21.23±2.91)ng/L;(10.59±2.96),(14.74±1.85)ng/L;P균＜0.01],차여정상대조조기본상사(P＞0.05).결론:뢰공등내지순가능통과대세포인자함량적조절치료관절염.
BACKGROUND: Common threewingnut root has the functions of anti-inflammation and immune inhibition, etc., and it has been used at present to treat various autoimmune diseases including rheumatoid arthritis.. Common threewingnut root has complex components, and triptolide is acknowledged as one of the important effective components of common threewingnut root.OBJECTIVE: To establish rat models of type Ⅱ collagen induced arthritis, and observe the effects of triptolide on the contents of interleukin-6,interleukin-10 and tumor necrosis factor alpha (TNF-α) in peripheral serum and synovial fluid.DESIGN: A randomized control animal experiment.SETTING: Department of Integrated Traditional and Western Medicine,Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology.MATERIALS: The experiments were carried out in the Tongji Hospital from November 2004 to July 2005. Fifty healthy male Wistar rats of clean degree were purchased from the experimental animal center of Tongji Medical College, Huazhong University of Science and Technology [qualification number of animal [scxk(E)2004-2007]. Triptolide (nobatch number because of temporary production) was bought from Fujian Institute of Medical Sciences, and the purity was above 98.5%.METHODS: ① Ten of the 50 rats were randomly selected as the normal controls, and the others were made into models. Type Ⅱ collagen emulsion was injected intradermally at five points along the back and tail of the rats,0.05 mL for each point, and injected intradermally at two points after 15 days. The rats in the normal control group were treated with saline in the same way. The effects of the model establishment were evaluated according to the scoring standards of arthritis index at 30 days after the first immunity, and the rats scored 6 points or above were taken as successful models and enrolled in the experiments. Twenty successful rat models were randomly divided into arthritis model group (n=10) and triptolide treated group (n=10). ② Triptolide (100 mg/L)was dispensed into parenteral solution with propylene glycol (0.05 in volume fraction), and then intramuscularly injected into hindlimb of rats in the triptolide treated group (0.04 mL/100 g), once every three days for 30 days. The rats in the normal control group were given isovolume saline, and those in the arthritis model group were treated with isovolume propylene glycol (0.05 in volume fraction). ③ The materials were removed at 30 days after administration. The contents of interleukin-6, interleukin-10 and TNF-α in peripheral serum and synovial fluid were detected with enzyme-linked immunosorbant assay(ELISA).MAIN OUTCOME MEASURES: The effects of triptolide on contents of TNF-α, interleukin-6 and interleukin-10 in peripheral serum and synovial fluid were observed.RESULTS: Fifty male Wistar rats of clean degree were selected, 10 were used as normal controls, and 20 of the other 40 rats were successfully made isto models and enrolled in the analysis of results. ① The TNF-α contents in peripheral serum and synovial fluid were the highest in the arthritis model group, and obviously decreased after treatment of triptolide [(35.09±8.82), (15.35±3.56) ng/L; (44.17±8.94), (22.54±4.76) ng/L; P＜ 0.01], which were similar to those in the normal control group (P ＞ 0.05).② The contents of interleukin-6 in peripheral serum and synovial fluid were the highest in the arthritis model group, and were obviously decreased after treatment of triptolide [(76.58 ±6.81), (42.45 ±5.72) rig/L;(88.69±10.56), (48.67±5.97) ng/L; P ＜ 0.01], but did not recover to the levels in the normal control group (P ＜ 0.05). ③ The contents of interleukin-10 in peripheral serum and synovial fluid were the lowest in the arthritis model group, and obviously increased after treatment of triptolide[(17.53±2.07), (21.23±2.91) ng/L; (10.59±2.96), (14.74±1.85) ng/L; P＜ 0.01], which were similar to those in the normal control group (P ＞ 0.05).CONCLUSION: Triptolide can treat arthritis by modulating the contents of cytokines.
