CAJ | 학술논문

目的肺表面活性物质蛋白D(SP-D)被认为是急性肺损伤(ALI)和急性呼吸窘迫综合征(ARDS)有价值的生物指标.该研究旨在探讨幼鼠ALl时及地塞米松干预后肺组织SP-D的变化.方法 144只SD大鼠被随机分为正常对照组、肺损伤组和地塞米松治疗组.腹腔内注射脂多糖(LPS,4 mg/kg)建立急性肺损伤模型,正常对照组注射等量生理盐水,治疗组于注射LPS 1小时后注射地塞米松(5 mg/kg).LPS注射后6,12,24,36,48,72 h每组各处死8只大鼠.用Western blot方法测定肺组织SP-D的相对含量.结果与正常对照组相比,ALl组在注射LPS后36,48,72 h SP-D含量明显下降(P＜0.01),在48 hrs达最低点(0.92±0.11 vs 3.27±0.52).地塞米松治疗组于注射LPS后36,48,72 h SP-D含量明显高于ALI组(P＜0.01),6,12,24,36和48 h与对照组相比差异无显著性,72 h差异有显著性(P＜0.05).结论急性肺损伤早期幼鼠肺组织SP-D含量降低.早期应用地塞米松能使ALI肺组织下降的SP-D明显上升.
목적 폐표면활성물질단백D(SP-D)피인위시급성폐손상(ALI)화급성호흡군박종합정(ARDS)유개치적생물지표.해연구지재탐토유서ALl시급지새미송간예후폐조직SP-D적변화.방법 144지SD대서피수궤분위정상대조조、폐손상조화지새미송치료조.복강내주사지다당(LPS,4 mg/kg)건립급성폐손상모형,정상대조조주사등량생리염수,치료조우주사LPS 1소시후주사지새미송(5 mg/kg).LPS주사후6,12,24,36,48,72 h매조각처사8지대서.용Western blot방법측정폐조직SP-D적상대함량.결과 여정상대조조상비,ALl조재주사LPS후36,48,72 h SP-D함량명현하강(P＜0.01),재48 hrs체최저점(0.92±0.11 vs 3.27±0.52).지새미송치료조우주사LPS후36,48,72 h SP-D함량명현고우ALI조(P＜0.01),6,12,24,36화48 h여대조조상비차이무현저성,72 h차이유현저성(P＜0.05).결론 급성폐손상조기유서폐조직SP-D함량강저.조기응용지새미송능사ALI폐조직하강적SP-D명현상승.
Objective Pulmonary surfactant protein-D (SP-D) is regarded as a valuable biomarker in acute lung injury (ALI) and acute respiratory distress syndrome (ARDS).This study was to explore the changes of SP-D content in lung tissue following ALI and the effect of dexamethasone (Dex) on the SP-D content in young rats.Methods One hundred and forty-four 21-day-old Sprague-Dawley rats were randomly assigned into control.ALI and Dex-treated groups.All was induced by intraperitoneal injection of lipopolysaccharide (LPS) (4 mg/kg) in the rats from the ALI and Dextreated groups.Normal saline was given for the control group.Dex(5 mg/kg) was administered 1 hr after LPS injection in the Dex-treated group.At each time interval of 6,12,24,36,48 and 72 hrs after LPS injection,eight rats of each group were randomly chosen and sacrificed.Western blot was employed to detect the content of SP-D in lung tissues.Results The pulmonary SP-D content decreased significantly at 36,48 and 72 hrs after LPS administration in the ALI group.and reduced to a nadir (0.92±0.11 vs 3.27±0.52) at 48 hrs compared with that of the control group (P＜0.01).The SPD content in the Dex-treated group increased significantly at 36,48 and 72 hrs after LPS administration when compared with the All group (P＜0.01).A significant difference in the SP-D content between the Dex-treated and the control group was noted only at 72 hrs after LPS administration (P＜0.05).Conclusions The SP-D content in lung tissue was reduced following ALI in young rats at the early stage.Early administration of Dex can significantly increase the pulmonary SP-D content.