中华损伤与修复杂志(电子版)
中華損傷與脩複雜誌(電子版)
중화손상여수복잡지(전자판)
Chinese Journal of Injury Repair and Wound Healing
2009年
2期
134-140
,共7页
张烨峰%郑锦标%白肃%杨丽娜%陈楚义%徐继庆
張燁峰%鄭錦標%白肅%楊麗娜%陳楚義%徐繼慶
장엽봉%정금표%백숙%양려나%진초의%서계경
脱细胞真皮基质%切口愈合%瘢痕%成纤维细胞
脫細胞真皮基質%切口愈閤%瘢痕%成纖維細胞
탈세포진피기질%절구유합%반흔%성섬유세포
acellular dermal matrix%wound healing%scar%fibroblast
目的 了解异体说细胞真皮基质(accelular dermal matrix,ADM)对SD大鼠皮肤切口愈合后瘢痕形成的影响.方法 4%NaOH溶液消蚀脱细胞法制备ADM,选用SD大鼠56只,每只大鼠背部脊柱两侧共做两道平行切口,建立皮肤切口模型.按自身对照分为实验组及对照组,实验组:将修剪成的ADM(3 cm×0.3 cm×0.1 cm)植入切口,丝线缝合切口;对照组则原位缝合.术后3天、5天、7天、14天、21天、28天、63天(每个时相点8只)处死大鼠,取切口皮肤组织做HE、Masson染色,进行生物组织学检测,RT-PCR方法检测各组切口中成纤维细胞内I型前胶原mRNA的表达水平.结果 术后实验组未见明显瘢痕组织,而对照组愈合后见明显线性瘢痕形成.镜下观察实验组切口中ADM逐渐被改建成自体组织,3天、5天、7天、14天及21天实验组中成纤维细胞数量较对照组减少(P<0.05);实验组切口中胶原的生成在各个时间点较对照组均减少,其中7天、14天、21天、28天及63天实验组切口中胶原的生成较对照组明显减少(P<0.01);RT-PCR检测组织中成纤维细胞内I型前胶原mRNA相对表达量对照组(0.809±0.042)比实验(0.540±0.026)增高.结论 ADM能抑制切口中成纤维细胞的增生和胶原的赳度分泌,减少瘢痕的形成.
目的 瞭解異體說細胞真皮基質(accelular dermal matrix,ADM)對SD大鼠皮膚切口愈閤後瘢痕形成的影響.方法 4%NaOH溶液消蝕脫細胞法製備ADM,選用SD大鼠56隻,每隻大鼠揹部脊柱兩側共做兩道平行切口,建立皮膚切口模型.按自身對照分為實驗組及對照組,實驗組:將脩剪成的ADM(3 cm×0.3 cm×0.1 cm)植入切口,絲線縫閤切口;對照組則原位縫閤.術後3天、5天、7天、14天、21天、28天、63天(每箇時相點8隻)處死大鼠,取切口皮膚組織做HE、Masson染色,進行生物組織學檢測,RT-PCR方法檢測各組切口中成纖維細胞內I型前膠原mRNA的錶達水平.結果 術後實驗組未見明顯瘢痕組織,而對照組愈閤後見明顯線性瘢痕形成.鏡下觀察實驗組切口中ADM逐漸被改建成自體組織,3天、5天、7天、14天及21天實驗組中成纖維細胞數量較對照組減少(P<0.05);實驗組切口中膠原的生成在各箇時間點較對照組均減少,其中7天、14天、21天、28天及63天實驗組切口中膠原的生成較對照組明顯減少(P<0.01);RT-PCR檢測組織中成纖維細胞內I型前膠原mRNA相對錶達量對照組(0.809±0.042)比實驗(0.540±0.026)增高.結論 ADM能抑製切口中成纖維細胞的增生和膠原的赳度分泌,減少瘢痕的形成.
목적 료해이체설세포진피기질(accelular dermal matrix,ADM)대SD대서피부절구유합후반흔형성적영향.방법 4%NaOH용액소식탈세포법제비ADM,선용SD대서56지,매지대서배부척주량측공주량도평행절구,건립피부절구모형.안자신대조분위실험조급대조조,실험조:장수전성적ADM(3 cm×0.3 cm×0.1 cm)식입절구,사선봉합절구;대조조칙원위봉합.술후3천、5천、7천、14천、21천、28천、63천(매개시상점8지)처사대서,취절구피부조직주HE、Masson염색,진행생물조직학검측,RT-PCR방법검측각조절구중성섬유세포내I형전효원mRNA적표체수평.결과 술후실험조미견명현반흔조직,이대조조유합후견명현선성반흔형성.경하관찰실험조절구중ADM축점피개건성자체조직,3천、5천、7천、14천급21천실험조중성섬유세포수량교대조조감소(P<0.05);실험조절구중효원적생성재각개시간점교대조조균감소,기중7천、14천、21천、28천급63천실험조절구중효원적생성교대조조명현감소(P<0.01);RT-PCR검측조직중성섬유세포내I형전효원mRNA상대표체량대조조(0.809±0.042)비실험(0.540±0.026)증고.결론 ADM능억제절구중성섬유세포적증생화효원적규도분비,감소반흔적형성.
Objective ADM was prepared by NaOH-maceration.This study Aimed to investigate the feasibility of the ADM inhibiting scar on the wound healing of SD rat and explore a modus operandi to prevent and cure the scar clinically.Metheds ADM was prepared by the combination of 4% NaOH sclution method.To establish the model of skin Wound,each rat was operated full-thicknessly in skin on two sides of back after haemostasis completely.All the wounds were assigned to two groups,one group was treated by transplantation of ADM,the other was interrupted suture.On days 3,5,7,14,21,28 and 63 after operation.the process of wound healing was observep to make post-operative evaluation, measure fibroblast and collagen content by HE and Masson staining,and measure the expression of type I procollagen mRNA from fibroblasts in all specimens by RT-PCR.Results After operation,scar was not found in the ADM experimental group,while linear scarring was found in the control group obviously Under light microscopy,ADM was rebuilt as autologous tissue gradually in wound, and inflammatory cell,contents of fibroblasts(P<0.05)and Collagens (P<0.01) were obviously less than controls.The epithelia and formation and thickness of the collagen looked like normal skin.And the expression of type I procollagens mRNA from fibroblasts also decreased in ADM experimental group.Conclusions Treated as autologous tissue, ADM decreased exudations, inflammatory reaction, and inhibited hyperplasy of fibroblasts and synthesis of collagens.ADM demonstrated advantages in remodeling dermal tissue and reduced scar tissue to near normal as a therapeutic tool.