海洋科学
海洋科學
해양과학
MARINE SCIENCES
2009年
12期
50-55
,共6页
贻贝属%16S rRNA%系统发育%单倍型
貽貝屬%16S rRNA%繫統髮育%單倍型
이패속%16S rRNA%계통발육%단배형
Mytilus%16s ribosome RNA%phylogeny%haplotype
通过比较贻贝属5个物种包括中国的两种贻贝的线粒体16S rRNA基因部分序列,来初步确定它们的系统发育关系和了解中国沿海两种贻贝的遗传多样性情况.以Perna viridis为外群,采用NJ法和MP法构建分子系统树.系统发育分析表明,5种贻贝(Mytilus californianus, M. corcuscus, M. galloprovincialis, M. edulis, M. trossulus)在系统树上依次进行分叉,呈放射状.M. californianus最为原始,M. corcuscus次之.每一个贻贝物种都形成单系.其中,M. edulis和M. trossulus是非常相似的,M. corcuscus和M. californianus的亲缘关系近.同时发现,我国沿海分布的紫贻贝(M. galloprovincialis)和厚壳贻贝(M. corcuscus)的遗传多样性都较高,但厚壳贻贝的遗传多样性要低于紫贻贝,可能是由于厚壳贻贝过度被渔民开采等导致厚壳贻贝群体大小降低的缘故.这里系统发育分析为将来进行物种进化、迁移和育种方面的比较研究提供理论基础.
通過比較貽貝屬5箇物種包括中國的兩種貽貝的線粒體16S rRNA基因部分序列,來初步確定它們的繫統髮育關繫和瞭解中國沿海兩種貽貝的遺傳多樣性情況.以Perna viridis為外群,採用NJ法和MP法構建分子繫統樹.繫統髮育分析錶明,5種貽貝(Mytilus californianus, M. corcuscus, M. galloprovincialis, M. edulis, M. trossulus)在繫統樹上依次進行分扠,呈放射狀.M. californianus最為原始,M. corcuscus次之.每一箇貽貝物種都形成單繫.其中,M. edulis和M. trossulus是非常相似的,M. corcuscus和M. californianus的親緣關繫近.同時髮現,我國沿海分佈的紫貽貝(M. galloprovincialis)和厚殼貽貝(M. corcuscus)的遺傳多樣性都較高,但厚殼貽貝的遺傳多樣性要低于紫貽貝,可能是由于厚殼貽貝過度被漁民開採等導緻厚殼貽貝群體大小降低的緣故.這裏繫統髮育分析為將來進行物種進化、遷移和育種方麵的比較研究提供理論基礎.
통과비교이패속5개물충포괄중국적량충이패적선립체16S rRNA기인부분서렬,래초보학정타문적계통발육관계화료해중국연해량충이패적유전다양성정황.이Perna viridis위외군,채용NJ법화MP법구건분자계통수.계통발육분석표명,5충이패(Mytilus californianus, M. corcuscus, M. galloprovincialis, M. edulis, M. trossulus)재계통수상의차진행분차,정방사상.M. californianus최위원시,M. corcuscus차지.매일개이패물충도형성단계.기중,M. edulis화M. trossulus시비상상사적,M. corcuscus화M. californianus적친연관계근.동시발현,아국연해분포적자이패(M. galloprovincialis)화후각이패(M. corcuscus)적유전다양성도교고,단후각이패적유전다양성요저우자이패,가능시유우후각이패과도피어민개채등도치후각이패군체대소강저적연고.저리계통발육분석위장래진행물충진화、천이화육충방면적비교연구제공이론기출.
In this paper we compared the partial sequences of the mitochondrial 16S ribosomal RNA of the five species of genus Mytilus including two species in China to primarily determine their phylogenetic relationships and estimate the genetic diversity of two species of Mytilus along the coast of China. The phylogenetic trees were reconstructed based on neighbor-joining and maximum parsimony methods. Perna viridis was used as an outgroup. The phylogenetic analysis revealed that five species of mussel Mytilus, M. californianus, M. corcuscus, M. galloprovincialis, M. edulis, M. trossulus, diverged in the basal polytomy at the base of the Mytilus radiation. M. californianus is the most basal among all species of genus M. ytilus, and the secondary is M. coruscus. The individuals of the each of five species solely cluster a monophyletic clade, respectively. M. edulis and M. trossulus are the most similar to the three closely related species. M. corcuscus is a closer relative of M. californianus than of M. edulis, M. galloprovincialis, M. trossulus. And we found that while the genetic diversity of both M. corcuscus and M. galloprovincialis distributed along the coast of China was higher, the genetic diversity of M. corcuscus was lower than that of M. galloprovincialis, this may be caused by declined population size due to overexploitation and human activity. This molecular phylogeny provides the theoretic foundation for future comparative studies of species evolution, immigration and breeding strategies.
