CAJ | 학술논문

目的:研究不同转移潜能骨肉瘤细胞差异表达的基因,筛选骨肉瘤转移相关的基因.方法: 分别提取低转移骨肉瘤细胞株M6和高转移骨肉瘤细胞株M8细胞总RNA,采用cDNA基因芯片筛查M6和M8细胞差异表达的基因,杂交信号用Generation Ⅲ array扫描,结果用Imagequant 5.0软件分析.选择基因芯片筛选到的典型差异表达基因用实时定量PCR进行验证.结果: M6和M8骨肉瘤细胞株差异表达的基因共有330个,其中与低转移组(M6)相比,在高转移组(M8)中显著性上调表达的基因有178个,下调表达的基因有152个.其中发生非常显著性上调表达的基因43个,发生非常显著性下调表达的基因49个.差异基因主要包括与细胞增殖相关的基因,提示与M8细胞增殖受抑相关;其次是与基因表达调控及信号转导相关的基因,提示这些基因和肿瘤转移有关联性.结论: 基因芯片方法对于筛选骨肉瘤转移相关基因有独特的优势,MG63细胞M8亚株中筛查到显著性上调的基因43个,显著性下调的基因49个,与骨肉瘤的转移有关.
목적:연구불동전이잠능골육류세포차이표체적기인,사선골육류전이상관적기인.방법: 분별제취저전이골육류세포주M6화고전이골육류세포주M8세포총RNA,채용cDNA기인심편사사M6화M8세포차이표체적기인,잡교신호용Generation Ⅲ array소묘,결과용Imagequant 5.0연건분석.선택기인심편사선도적전형차이표체기인용실시정량PCR진행험증.결과: M6화M8골육류세포주차이표체적기인공유330개,기중여저전이조(M6)상비,재고전이조(M8)중현저성상조표체적기인유178개,하조표체적기인유152개.기중발생비상현저성상조표체적기인43개,발생비상현저성하조표체적기인49개.차이기인주요포괄여세포증식상관적기인,제시여M8세포증식수억상관;기차시여기인표체조공급신호전도상관적기인,제시저사기인화종류전이유관련성.결론: 기인심편방법대우사선골육류전이상관기인유독특적우세,MG63세포M8아주중사사도현저성상조적기인43개,현저성하조적기인49개,여골육류적전이유관.
Objective: To investigate the differentially expressed genes in osteosarcoma cell lines with various metastatic potentialities, and to screen for new candidate genes related to metastasis of osteosarcomas. Methods: The total RNAs of a lowly metastatic and a highly metastatic osteosarcoma cell lines (M6 and M8) were extracted. Differentially expressed genes in the two osteosarcoma cell lines were studied by cDNA microarray. The hybridization signals were scanned with a Generation Ⅲ array scanner and analyzed by Imagequant 5.0 software. Typical differentially expressed genes were further verified by real-time quantitative PCR. Results: There were 330 differentially expressed genes between M6 and M8 cells. In the high-metastasis M8 cells, 178 genes were up-regulated and 152 genes were down-regulated compared to the low-metastasis M6 cells, with 43 extremely up-regulated and 49 extremely down-regulated. The differentially expressed genes were mainly associated with cell proliferation, indicating these genes might be related to the inhibition of M6 cells. Other differentially expressed genes included those associated with the regulation of gene expression and signal transduction, indicating these genes might be correlated with tumor metastasis. Conclusion: cDNA microarray shows an advantage in identifying genes associated with metastasis of osteosarcoma. In M8 subset of MG63 osteosarcoma cells,43 genes are up-regulated and 49 genes are down-regulated, which may be related with metastasis of osteosarcoma.