CAJ | 학술논문

采用胃蛋白酶水解黄鲫,测定酶解液的蛋白提取率、可溶性肽含量、水解度及氨基酸组成,并对酶解液体外抗氧化和抑菌作用进行研究.结果表明:黄鲫胃蛋白酶酶解液的蛋白提取率、可溶性肽提取率、水解度分别为(110.28±4.81)%、(81.78±0.04)%和(18.12±0.39)%,黄鲫胃蛋白酶酶解液的人体必需氨基酸相对含量可达37.91%.黄鲫胃蛋白酶酶解液具有很强的抗氧化能力,清除羟自由基和DPPH自由基的ED50分别为4.55μg/mL和4.46μg/mL,还原力随着黄鲫胃蛋白酶酶解液质量浓度的增加而增大,在3.4 μg/mL和13.6μg/mL低、中质量浓度时螯合金属离子能力与EDTA接近.体外抑菌实验显示:黄鲫胃蛋白酶酶解液具有广谱抑菌性,对大肠杆菌的抑菌效力分别与13.21～15.44mcg/mL的氨苄肯霉素和325.00～340.00U/mL的硫酸链霉素相当(P＞0.05).
채용위단백매수해황즉,측정매해액적단백제취솔、가용성태함량、수해도급안기산조성,병대매해액체외항양화화억균작용진행연구.결과표명:황즉위단백매매해액적단백제취솔、가용성태제취솔、수해도분별위(110.28±4.81)%、(81.78±0.04)%화(18.12±0.39)%,황즉위단백매매해액적인체필수안기산상대함량가체37.91%.황즉위단백매매해액구유흔강적항양화능력,청제간자유기화DPPH자유기적ED50분별위4.55μg/mL화4.46μg/mL,환원력수착황즉위단백매매해액질량농도적증가이증대,재3.4 μg/mL화13.6μg/mL저、중질량농도시오합금속리자능력여EDTA접근.체외억균실험현시:황즉위단백매매해액구유엄보억균성,대대장간균적억균효력분별여13.21～15.44mcg/mL적안변긍매소화325.00～340.00U/mL적류산련매소상당(P＞0.05).
Half-fin anchovy(Setipirma taty)homogenate was hydrolysated by pepsin.Protein yield,contents of soluble peptides and degree of hydrolysis were analyzed and in vitro antioxidant and antibacterial activities of the hydrolysates were also evaluated.Results showed protein extract rate,soluble peptides contents,degree of hydrolysis of half-fin anchovy pepsin hydrolysate were(1 10±4.81)%,(81.78±0.04)%and(18.12±0.39)%,respectively.The relative contents of essential amino acids in half-fin anchovy hydrolysate(HAH)reached 37.9 1%.HAH showed strong radical scavenging activity against hydroxyl and DPPH radicals with the EDso values of 4.55μg/mL and 4.46μg/mL,respectively.The reducing power increased with rising concentration and the metal chelating ability was equivalent to that of EDTA at the 3.4μg/mL or 1 3.6μg/mL.HAH displayed broad antibacterial spectra in vitro and the antibacterial effecacy against Escherichia coli was equal to 13.21--15.44 mcg/mL of ampicillin of and 325.00-340.00 U/mL of streptomycin sulfate(P＞0.05).