CAJ | 학술논문

目的建立络气郁滞证大鼠动物模型并进行评价.方法对大鼠慢性束缚6周建立络气郁滞证模型,观察模型大鼠的精神、饮食量、体质量、蔗糖水摄取量,血清皮质酮(CORT)、血浆内皮素(ET),血清5羟色胺(5-HT)、NO以及主动脉组织内皮细胞超微结构的变化.结果络气郁滞证模型表现为精神萎靡,反应迟钝,摄食量减少,体质量增长缓慢,蔗糖水摄取量、摄食量及体重均明显低于对照组(P<0.05或P<0.01);模型组大鼠血浆ET升高、血清CORT升高,5-HT、NO降低(P<0.05或P<0.01);内皮细胞超微结构发生明显改变.结论利用慢性束缚法6周,通过观测大鼠体质量、摄食量、蔗糖水摄取量、CORT、ET、NO、5-HT的变化,初步建立络气郁滞证大鼠动物模型,制作方法简单,评价指标客观,为脉络血管系统病的研究提供良好的实验基础.
목적 건립락기욱체증대서동물모형병진행평개.방법 대대서만성속박6주건립락기욱체증모형,관찰모형대서적정신、음식량、체질량、자당수섭취량,혈청피질동(CORT)、혈장내피소(ET),혈청5간색알(5-HT)、NO이급주동맥조직내피세포초미결구적변화.결과 락기욱체증모형표현위정신위미,반응지둔,섭식량감소,체질량증장완만,자당수섭취량、섭식량급체중균명현저우대조조(P<0.05혹P<0.01);모형조대서혈장ET승고、혈청CORT승고,5-HT、NO강저(P<0.05혹P<0.01);내피세포초미결구발생명현개변.결론 이용만성속박법6주,통과관측대서체질량、섭식량、자당수섭취량、CORT、ET、NO、5-HT적변화,초보건립락기욱체증대서동물모형,제작방법간단,평개지표객관,위맥락혈관계통병적연구제공량호적실험기출.
Objective To establish and evaluate the rat animal model with collateral qi stasis. Methods To establish animal model with collateral qi stasis in 6 weeks by chronic constraint.To observe the changes of consciousness, appetite, body weight, the intake of cane sugar water, and the levels of CORT, ET, NO, 5-HT. The ultramicrostructure change of aorta was observed by transmission electron microscope. Results The animal model rats showed deprementia, dull,no appetite,increasing slow of body weight,the cane sugar water intake was lowered significantly (P<0.01 or P<0.05),the levels of CORT,HCY,ET were increased significantly(P<0.01 or P<0.05),and the levels of 5-HT,NO were lowered significantly (P<0.01 or P<0.05). The ultramicrostructure of aorta changed obviously.Conclusion The animal model with collateral qi stasis is established with chronic constraint. The method has been succeed through observation the changes of body weight, cane sugar water intake, appetite, CORT,ET,NO,5-HT, and ultramicrostructure of aorta. The method is easy and the evaluated indexes are objective, which provide the good experiment foundation for the study of vessel-collateral and vascular system disorders.