中国法医学杂志
中國法醫學雜誌
중국법의학잡지
CHINESE JOURNAL OF FORENSIC MEDICINE
2010年
2期
87-90,93
,共5页
法医毒物分析%死后分布%中毒%气相色谱/质谱联用%甲氰菊酯
法醫毒物分析%死後分佈%中毒%氣相色譜/質譜聯用%甲氰菊酯
법의독물분석%사후분포%중독%기상색보/질보련용%갑청국지
forensic toxicological analysis%postmortem distribution%poisoning%GC/MS%fenpropathrin
目的 建立甲氰菊酯家兔灌胃染毒致死模型和生物检材中甲氰菊酯的气相色谱和气相色谱-质谱联用检测方法 ,研究甲氰菊酯在家兔体内的死后分布规律.方法 家兔6只,甲氰菊酯经口灌胃染毒,死亡后迅速解剖,取心血、外周血、肝等组织,气相色谱和气相色谱-质谱联用法检测甲氰菊酯含量;部分组织经甲醛固定,HE染色,光镜观察其病理改变.结果 家兔染毒后2～3h出现中毒表现,染毒后4.5～8h死亡.气相色谱和气相色谱-质谱联用法均检测到甲氰菊酯.甲氰菊酯在家兔体内死后分布为胃壁(458.92±32.82)μg/g、肾(46.47±6.30)μg/g、肝(35.79±20.11)μg/g、大脑(28.77±10.52)μg/g、心(26.49±4.10)μg/g、脾(22.23±5.37)μg/g、胆汁(10.87±1.42)μg/mL、肺(10.32±0.78)μg/g、周围血(8.14±1.12)μg/mL和心m(8.20±1.83)μg/mL.结论 甲氰菊酯的灌胃染毒致死模型、气相色谱和气相色谱-质谱联用检测方法 及死后分布规律可应用于甲氰菊酯中毒死亡案件的法医学鉴定及法医毒物动力学研究.
目的 建立甲氰菊酯傢兔灌胃染毒緻死模型和生物檢材中甲氰菊酯的氣相色譜和氣相色譜-質譜聯用檢測方法 ,研究甲氰菊酯在傢兔體內的死後分佈規律.方法 傢兔6隻,甲氰菊酯經口灌胃染毒,死亡後迅速解剖,取心血、外週血、肝等組織,氣相色譜和氣相色譜-質譜聯用法檢測甲氰菊酯含量;部分組織經甲醛固定,HE染色,光鏡觀察其病理改變.結果 傢兔染毒後2～3h齣現中毒錶現,染毒後4.5～8h死亡.氣相色譜和氣相色譜-質譜聯用法均檢測到甲氰菊酯.甲氰菊酯在傢兔體內死後分佈為胃壁(458.92±32.82)μg/g、腎(46.47±6.30)μg/g、肝(35.79±20.11)μg/g、大腦(28.77±10.52)μg/g、心(26.49±4.10)μg/g、脾(22.23±5.37)μg/g、膽汁(10.87±1.42)μg/mL、肺(10.32±0.78)μg/g、週圍血(8.14±1.12)μg/mL和心m(8.20±1.83)μg/mL.結論 甲氰菊酯的灌胃染毒緻死模型、氣相色譜和氣相色譜-質譜聯用檢測方法 及死後分佈規律可應用于甲氰菊酯中毒死亡案件的法醫學鑒定及法醫毒物動力學研究.
목적 건립갑청국지가토관위염독치사모형화생물검재중갑청국지적기상색보화기상색보-질보련용검측방법 ,연구갑청국지재가토체내적사후분포규률.방법 가토6지,갑청국지경구관위염독,사망후신속해부,취심혈、외주혈、간등조직,기상색보화기상색보-질보련용법검측갑청국지함량;부분조직경갑철고정,HE염색,광경관찰기병리개변.결과 가토염독후2～3h출현중독표현,염독후4.5～8h사망.기상색보화기상색보-질보련용법균검측도갑청국지.갑청국지재가토체내사후분포위위벽(458.92±32.82)μg/g、신(46.47±6.30)μg/g、간(35.79±20.11)μg/g、대뇌(28.77±10.52)μg/g、심(26.49±4.10)μg/g、비(22.23±5.37)μg/g、담즙(10.87±1.42)μg/mL、폐(10.32±0.78)μg/g、주위혈(8.14±1.12)μg/mL화심m(8.20±1.83)μg/mL.결론 갑청국지적관위염독치사모형、기상색보화기상색보-질보련용검측방법 급사후분포규률가응용우갑청국지중독사망안건적법의학감정급법의독물동역학연구.
Objective To develop a rabbit model of fenpropathrin poisoning by intragastric administra-tion, to establish a method for qualitative and quantitative analysis of fenpropathrin in organs and fluid in rab-bits, and investigate the postmortem distribution of fenpropathrin in poisoning death rabbits. Methods Six rab-bits died from fenpropathrin poisoning were dissected as soon as their vital signs disappeared after intoxication. The samples of heart blood, peripheral blood, bile, liver, kidney and so on were collected. Analysis was per-formed with a GC equipped with a NPD and a GC-MS. Microscopic examination was also conducted in HE-stained tissue sections. Results Poisoning signs were observed 2 ～ 3h after fenpropathrin delivery. Deaths oc-curred in all rabbits at 4. 5 ～ 8h after intoxication. Fenpropathrin eould be detected in all of the samples ex-cept for in urine by GC or GC-MS with highest concentration in the gastric wall (458.92±32.82)μg/g, fol-lowed by kidney ( 46. 47 ± 6. 30 ) μg/g, liver ( 35.79 ±20. 11 ) μg/g, brain ( 28.77±10. 52 ) μg/g, heart (26. 49±4. 10 )μg/g, spleen ( 22.23±5.37 ) μg/g, bile ( 10.87±1.42 )μg/mL, lung ( 10. 32±0.78 )μg/g, peripheral blood ( 8. 14 ± 1.12 ) μg/mL and heart blood ( 8.20 ±1.83 )μg,/mL. Conclusion Fenpropathrin in biological specimens can be determined by GC equipped with a NPD or GC-MS. The poison-ing death animal model, the analysis and the postmortem distribution data of fenpropathrin can be applied to the forensic identification of fenpropathrin poisoning death .
