CAJ | 학술논문

目的检测ANP大鼠过氧化酶体增殖物激活受体γ(PPARγ)mRNA和蛋白表达的变化,探讨其意义.方法 36只SD大鼠按完全随机法分成对照组和ANP组.于术后3 h、6 h、12 h处死大鼠,分别检测血清淀粉酶含量,观察胰腺组织大体及镜下病理学改变,并采用RT-PCR和免疫组化分别检测胰腺PPARγmRNA和蛋白的表达.结果 ANP组术后6 h血清淀粉酶、胰腺大体及镜下病理分值分别为(7170.83±1635.59)U/L、6.67±1.03和13.00±2.36,均显著高于对照组(P<0.01);PPARγmRNA相对表达量为0.18±0.05,与对照组的0.22±0.03无显著差异;PPARγ蛋白相对表达量为4.17±0.98.较对照组的1.83±0.71显著升高(P<0.05).结论 ANP时PPARγ mRNA无明显下降,而PPARr蛋白明显增加.表明炎症损伤时,失活状态的PPARγ增多,同时反馈性抑制了PPARγ基因的表达.
목적 검측ANP대서과양화매체증식물격활수체γ(PPARγ)mRNA화단백표체적변화,탐토기의의.방법 36지SD대서안완전수궤법분성대조조화ANP조.우술후3 h、6 h、12 h처사대서,분별검측혈청정분매함량,관찰이선조직대체급경하병이학개변,병채용RT-PCR화면역조화분별검측이선PPARγmRNA화단백적표체.결과 ANP조술후6 h혈청정분매、이선대체급경하병리분치분별위(7170.83±1635.59)U/L、6.67±1.03화13.00±2.36,균현저고우대조조(P<0.01);PPARγmRNA상대표체량위0.18±0.05,여대조조적0.22±0.03무현저차이;PPARγ단백상대표체량위4.17±0.98.교대조조적1.83±0.71현저승고(P<0.05).결론 ANP시PPARγ mRNA무명현하강,이PPARr단백명현증가.표명염증손상시,실활상태적PPARγ증다,동시반궤성억제료PPARγ기인적표체.
Objective To investigate the changes of PPARr mRNA and protein expressions in rats with ANP. Methods 36 rats were randomly divided into sham group and ANP group. The rats were sacrificed 3 h, 6 h,12 h after ANP induction, the levels of serum amylase were measured, the pancreatic pathological changes were determined and the expressions of pancreatic PPARr mRNA and protein was examined by RT- PCR and immunohistochemistry. Results In ANP group, the level of serum amylase at 6h was (7170.83± 1635.59) U/L, the scores of pathological changes were 6.67±1.03 and 13.00±2.36, which were much higher than those of sham group (P<0.01) ; the PPARr mRNA expression was 0.18±0.05, and there were no obvious differences compared with that of sham group (0.22±0.03 ) ; PPARr protein expression was 4.17 ±0.98, which was significantly higher than that of sham group (1.83±0.71, P<0.05). Conclusions Inflammatory injury resulted in increased deactivation of pancreatic acinar PPARr, meanwhile PPARr gene expression was inhibited by feedback.