CAJ | 학술논문

目的对CP大鼠离体胰腺组织块进行高分辨磁共振波谱分析,探索其代谢变化特征.方法 SD大鼠30只,数字随机法分为实验组(20只)与对照组(10只).实验组经尾静脉注射二丁基二氯化物8 mg/kg体重,对照组注射等量溶剂.胰腺组织行病理检查及胶原纤维染色.运用高分辨魔角旋转磁共振波谱技术对离体大鼠CP组织样品代谢物进行分析.结果造模60 d后,11只大鼠胰腺小叶结构破坏,腺泡消失,炎症细胞广泛浸润,纤维组织大量增生,广泛间质纤维化,呈典型CP表现.波谱分析显示CP组织的磷酸胆碱(PC)和甘油磷酸胆碱(GPC)、牛黄酸(Tan)及乳酸(Lac)峰强度增加;甜菜碱(Bet)、谷氨酸(Glu)、丙氨酸(Ala)、异亮氨酸(Ile)、亮氨酸(Leu)、缬氨酸(Val)峰降低;而乙酸(Ace)、胆碱(Cho)峰强度则无明显差异.结论离体大鼠CP组织具有显著的代谢特征,为人CP波谱分析研究奠定了实验基础.
목적 대CP대서리체이선조직괴진행고분변자공진파보분석,탐색기대사변화특정.방법 SD대서30지,수자수궤법분위실험조(20지)여대조조(10지).실험조경미정맥주사이정기이록화물8 mg/kg체중,대조조주사등량용제.이선조직행병리검사급효원섬유염색.운용고분변마각선전자공진파보기술대리체대서CP조직양품대사물진행분석.결과 조모60 d후,11지대서이선소협결구파배,선포소실,염증세포엄범침윤,섬유조직대량증생,엄범간질섬유화,정전형CP표현.파보분석현시CP조직적린산담감(PC)화감유린산담감(GPC)、우황산(Tan)급유산(Lac)봉강도증가;첨채감(Bet)、곡안산(Glu)、병안산(Ala)、이량안산(Ile)、량안산(Leu)、힐안산(Val)봉강저;이을산(Ace)、담감(Cho)봉강도칙무명현차이.결론 리체대서CP조직구유현저적대사특정,위인CP파보분석연구전정료실험기출.
Objective To investigate the metabolite features of chronic pancreatitis in rats in vitro by high resolution magic angle spinning nuclear magnetic resonance spectroscopy. Methods A total of 30 Spraque-Dawley rats were randomized into experimental group (n =20) and control group (n = 10). All the animals in experimental group were intravenously injected with 8 mg/kg body weight DBTC, and the animals in the control group received same amount of solvent. Pancreatic tissues were examined by histology and Van Gieson staining. Metabolic changes of chronic pancreatitis in vitro in rats were studied by high resolution magic angle spinning nuclear magnetic resonance spectroscopy. Results 60 days after DBTC application, the pancreatic tissue was characterized by an extended interstitial fibrosis with infiltrating mononuclear cells. Compared with the control group, the signal intensities of phosphocholine (Pc) and glycerophosphocholine (Gpc), taurine (Tau), lactate (Lac) of chronic pancreatitis group increased. Oppositely, the signal intensities of betine (Bet), glutamic acid ( Glu ), alanine (Ala), ileucine (He), leucine ( Leu ) and valine (Val) decreased. The signal intensities of acetic acid (Ace) and choline (Cho) were not changed. Conclusions There were obvious metabolic features of chronic pancreatitis in rats, and it is helpful for the application of magnetic resonance spectroscopy in chronic pancreatitis in human studies.