CAJ | 학술논문

目的利用变形链球菌luxS基因敲除突变株,研究这一种属间密度感应系统对生物膜早期形成的影响.方法通过在生物膜培养悬液中加入与菌细胞直径相近的磁性小珠,利用这些小珠在磁场中受到生物膜的位移约束力的原理,采用生物膜定量分析仪,定量比较、分析变形链球菌luxS基因knockout突变株与野生株在生物膜形成上的差异.结果变形链球菌luxS基因突变株与野生株在生物膜形成模式上有显著差别,突变株生物膜自第6小时起开始形成,生物膜形成指数(biofilm index,BFI)差值ABFI=2.015,约第10小时突变株形成的生物膜可完全限制磁珠在磁场中的位移(ABFI=7.025);而野生株生物膜约第10小时开始形成(ABFI=1.875),明显晚于突变株.12h后两菌株生物膜形成未见明显差异(P>0.05).结论变形链球菌luxS基因的缺失可影响生物膜的早期形成.
목적 이용변형련구균luxS기인고제돌변주,연구저일충속간밀도감응계통대생물막조기형성적영향.방법통과재생물막배양현액중가입여균세포직경상근적자성소주,이용저사소주재자장중수도생물막적위이약속력적원리,채용생물막정량분석의,정량비교、분석변형련구균luxS기인knockout돌변주여야생주재생물막형성상적차이.결과변형련구균luxS기인돌변주여야생주재생물막형성모식상유현저차별,돌변주생물막자제6소시기개시형성,생물막형성지수(biofilm index,BFI)차치ABFI=2.015,약제10소시돌변주형성적생물막가완전한제자주재자장중적위이(ABFI=7.025);이야생주생물막약제10소시개시형성(ABFI=1.875),명현만우돌변주.12h후량균주생물막형성미견명현차이(P>0.05).결론변형련구균luxS기인적결실가영향생물막적조기형성.
Objective To investigate the effect of Streptococcus mutans luxS mutantation on the early biofilm formatien. Methods Based on the immobilization of magnetic beads by adherent cells, an assay of biofilm quantitative analysis was developed for the kinetic quantification of biofilm formation in this study. Streptococcus mutans luxS mutant strain was constructed and subject to this biofilm luxS mutant strain were compared. Results The AluxS mutant started to form a biofilm from the 6 th hour(△BFI=2.015 ), and the △BFI of luxS mutant increased more quickly than that of the wild type strain, until reaching a complete immobilization of the beads after 10 hours(△BFI =7. 025). The wild-type strain start to form a biofilm from the 10 th hour(△BFI = 1. 875) and the beads were completely immobilized between 12 and 14 hours. Conclusions The luxS mutation can accelerate biofilm on a polystyrene surface during the mid-exponential growth phase. And a luxS-dependent signal may play an important role in the early biofilm formation of Streptococcus mutans.