CAJ | 학술논문

目的构建包含H5N1-HA基因的重组腺病毒疫苗并探讨其免疫效果.方法用Admax系统构建包含H5N1-HA基因的重组腺病毒疫苗,并用PCR、Western-Blot等方法对重组病毒疫苗进行鉴定;疫苗免疫小鼠后,通过HI实验和ELISPOT实验检测其体液免疫和细胞免疫反应,评价其免疫效果.结果成功得到了含有H5N1-HA基因的重组腺病毒疫苗;基因表达鉴定表明,HA基因能够在细胞中进行表达;血凝抑制实验结果显示小鼠产生的针对HA抗体滴度在1:320和1:640之间;ELISPOT结果显示实验组和对照组(PBS)相比斑点数量差异有统计学意义(P<0.05),以上免疫结果表明重组腺病毒载体疫苗可以诱导小鼠产生良好的特异性体液和细胞免疫反应.结论含H5NA-HA的重组腺病毒疫苗可以诱导小鼠产生良好的免疫反应,为研制人禽流感疫苗打下基础.
목적 구건포함H5N1-HA기인적중조선병독역묘병탐토기면역효과.방법 용Admax계통구건포함H5N1-HA기인적중조선병독역묘,병용PCR、Western-Blot등방법대중조병독역묘진행감정;역묘면역소서후,통과HI실험화ELISPOT실험검측기체액면역화세포면역반응,평개기면역효과.결과 성공득도료함유H5N1-HA기인적중조선병독역묘;기인표체감정표명,HA기인능구재세포중진행표체;혈응억제실험결과현시소서산생적침대HA항체적도재1:320화1:640지간;ELISPOT결과현시실험조화대조조(PBS)상비반점수량차이유통계학의의(P<0.05),이상면역결과표명중조선병독재체역묘가이유도소서산생량호적특이성체액화세포면역반응.결론 함H5NA-HA적중조선병독역묘가이유도소서산생량호적면역반응,위연제인금류감역묘타하기출.
Objective To construct adenovirus vector vaccine against H5N1 influenza virus and study on the immunogeuicity. Methods In this study, we amplified hemagglutinin (HA) gene sequence of H5N1 influenza virus (A/Anhui/1/2005), then constructed an adenovirus vector vaccine (Adv-HA), followed by tests in BALB/c mice for the immunogenicity with the vaccine and immunization strategies. Results The recombinate Adv-HA vaccine could effectively induce both humoral and cellular immunity against human H5N1 influenza virus. Conclusion The Adv-HA vaccination against H5N1 influenza is a potential strategy and worthy of further investigation.