中华神经医学杂志
中華神經醫學雜誌
중화신경의학잡지
CHINESE JOURNAL OF NEUROMEDICINE
2011年
3期
251-254
,共4页
白润涛%韩漫夫%周艳霞%程文蕾
白潤濤%韓漫伕%週豔霞%程文蕾
백윤도%한만부%주염하%정문뢰
中毒%S100B%髓鞘碱性蛋白
中毒%S100B%髓鞘堿性蛋白
중독%S100B%수초감성단백
Poisoning%S100B%Myelin basic protein
目的 研究急性液化石油气(LPG)中毒大鼠血清S100B和髓鞘碱性蛋白(MBP)的变化.方法 健康雄性成年SD大鼠54只按随机数字表法分为正常对照组(n=6)、20%LPG中毒组(n=24)和50%LPG中毒组(n=24).后2组大鼠分别吸入20%、50%LPG,对照组吸入等体积的空气.在中毒1、2、3、7 d时每组取6只大鼠进行神经功能缺损评分(NSS),ELISA法检测血清样本S100B和MBP的含量.结果 与正常对照组比较,20%LPG组中毒1 d、50%LPG组中毒1 d和2 d时NSS评分均较高,差异有统计学意义(P＜0.05);在1 d和2 d时,20%LPG组和50%LPG组大鼠血清S100B、MBP均高于正常对照组,且50%LPG组高于20%LPG组,差异有统计学意义(P＜0.05);3 d时50%LPG组血清S100B、MBP高于正常对照组和20%LPG组,差异有统计学意义(P＜0.05);大鼠LPG中毒1 d时NSS评分、血清S100B、MBP浓度最高,与其它时间点比较差异均有统计学意义(P＜0.05),后随时间延长逐渐恢复.结论 LPG中毒大鼠血清S100B和MBP同时升高,提示胶质细胞参与LPG中毒对神经系统的损害.
目的 研究急性液化石油氣(LPG)中毒大鼠血清S100B和髓鞘堿性蛋白(MBP)的變化.方法 健康雄性成年SD大鼠54隻按隨機數字錶法分為正常對照組(n=6)、20%LPG中毒組(n=24)和50%LPG中毒組(n=24).後2組大鼠分彆吸入20%、50%LPG,對照組吸入等體積的空氣.在中毒1、2、3、7 d時每組取6隻大鼠進行神經功能缺損評分(NSS),ELISA法檢測血清樣本S100B和MBP的含量.結果 與正常對照組比較,20%LPG組中毒1 d、50%LPG組中毒1 d和2 d時NSS評分均較高,差異有統計學意義(P＜0.05);在1 d和2 d時,20%LPG組和50%LPG組大鼠血清S100B、MBP均高于正常對照組,且50%LPG組高于20%LPG組,差異有統計學意義(P＜0.05);3 d時50%LPG組血清S100B、MBP高于正常對照組和20%LPG組,差異有統計學意義(P＜0.05);大鼠LPG中毒1 d時NSS評分、血清S100B、MBP濃度最高,與其它時間點比較差異均有統計學意義(P＜0.05),後隨時間延長逐漸恢複.結論 LPG中毒大鼠血清S100B和MBP同時升高,提示膠質細胞參與LPG中毒對神經繫統的損害.
목적 연구급성액화석유기(LPG)중독대서혈청S100B화수초감성단백(MBP)적변화.방법 건강웅성성년SD대서54지안수궤수자표법분위정상대조조(n=6)、20%LPG중독조(n=24)화50%LPG중독조(n=24).후2조대서분별흡입20%、50%LPG,대조조흡입등체적적공기.재중독1、2、3、7 d시매조취6지대서진행신경공능결손평분(NSS),ELISA법검측혈청양본S100B화MBP적함량.결과 여정상대조조비교,20%LPG조중독1 d、50%LPG조중독1 d화2 d시NSS평분균교고,차이유통계학의의(P＜0.05);재1 d화2 d시,20%LPG조화50%LPG조대서혈청S100B、MBP균고우정상대조조,차50%LPG조고우20%LPG조,차이유통계학의의(P＜0.05);3 d시50%LPG조혈청S100B、MBP고우정상대조조화20%LPG조,차이유통계학의의(P＜0.05);대서LPG중독1 d시NSS평분、혈청S100B、MBP농도최고,여기타시간점비교차이균유통계학의의(P＜0.05),후수시간연장축점회복.결론 LPG중독대서혈청S100B화MBP동시승고,제시효질세포삼여LPG중독대신경계통적손해.
Objective To observe the changes of serum S100B and MBP in rats with liquid petroleum gas poisoning. Methods Fifty-four healthy adult male SD rats were randomized into normal control group (n=6), 20% LPG poisoning group (n=24) and 50% LPG poisoning group (n=24). Rat models of liquid petroleum gas poisoning were established in the later 2 groups, and controls were given the same volume of air. The rats of each group(n=6) were scored with neurological severity scale (NSS) and the blood serum was collected on the 1st, 2nd, 3nd and 7th d of poisoning, respectively. The levels of S100B and MBP were detected by euzymelinked immunosorbent assay (ELISA).Results As compared with the scores of NSS in the normal control group, those on the 1st d of poisoning in the 20% LPG poisoning group and those on the 1st and 2nd d of poisoning in the 50% LPG poisoning group were significantly higher (P＜0.05). The levels of Sl00B and MBP in 20% and 50% LPG poisoning groups were higher than those in the control group on the 1st and 2nd d of poisoning (P＜0.05); the levels of S100B and MBP in 50% LPG poisoning group were higher than those in 20% LPG poisoning group on the 1st and 2nd d of poisoning (P＜0.05). The levels of S 100B and MBP in 50% LPG poisoning group were higher than those in 20% LPG poisoning group and normal control group on the 3rd of poisoning (P＜0.05). The NSS scores and the levels of S100B and MBP in rats with LPG poisoning enjoyed the highest scores or levels on the 1st d of poisoning and those decreased after that. Conclusion The levels of S100B and MBP of rats with LPG poisoning increase, indicating that gliocytes participate in the mechanism of nervous system injury in rats with liquid petroleum gas poisoning.
