CAJ | 학술논문

研究了γ射线照射同时吸入苯、甲苯和CO产生的复合效应,并分析了这四个因素之间相互作用的性质(拮抗、相加或协同).90只家兔分成9组,按L8(27)正交表设计进行四因素二水平的8组实验,另一组为对照组.各因素的两种暴露水平分别是:γ射线为0.0075Gy/d和0.0375Gy/d;苯为4015mg/m3和18233mg/m3;甲苯为9030mg/m3和40768mg/m3;CO为934mg/m3和2788mg/m3.暴露实验在密闭舱内进行,每天2h,每周5d,持续8周.对实验结果用方差分析和组间比较进行了处理.结果:(1)相互作用因子,即四个因素的联合作用效应与四个因素单独作用产生的效应总和的比值(ω),对淋巴细胞的双着丝点加环、无着丝粒、畸变细胞、总畸变、微核及SCEs/C分别为2.16、1.58、2.07、2.67、1.31和1.18,对畸形精子率则高达 5.97.这些数值显示各因素之间的相互作用主要是协同作用(ω>1),但苯和射线结合对淋巴细胞无着丝粒为桔抗作用.(2)γ射线、苯、甲苯和CO四个因素都引起睾丸重量指数降低;γ射线、甲苯和CO能引起精子数减少和畸形精子率增高;γ射线与甲苯对降低睾丸重量指数和增高畸形精子率有显著的协同性交互作用.(3)CO能导致白细胞总数、血小板及血红蛋白显著减少,甲苯能使淋巴细胞减少.(4)γ射线、苯和甲苯对诱发某些生物效应具有很显著的协同作用;CO在与其它三个因素同时作用时对性腺能引起显著的影响.
연구료γ사선조사동시흡입분、갑분화CO산생적복합효응,병분석료저사개인소지간상호작용적성질(길항、상가혹협동).90지가토분성9조,안L8(27)정교표설계진행사인소이수평적8조실험,령일조위대조조.각인소적량충폭로수평분별시:γ사선위0.0075Gy/d화0.0375Gy/d;분위4015mg/m3화18233mg/m3;갑분위9030mg/m3화40768mg/m3;CO위934mg/m3화2788mg/m3.폭로실험재밀폐창내진행,매천2h,매주5d,지속8주.대실험결과용방차분석화조간비교진행료처리.결과:(1)상호작용인자,즉사개인소적연합작용효응여사개인소단독작용산생적효응총화적비치(ω),대림파세포적쌍착사점가배、무착사립、기변세포、총기변、미핵급SCEs/C분별위2.16、1.58、2.07、2.67、1.31화1.18,대기형정자솔칙고체 5.97.저사수치현시각인소지간적상호작용주요시협동작용(ω>1),단분화사선결합대림파세포무착사립위길항작용.(2)γ사선、분、갑분화CO사개인소도인기고환중량지수강저;γ사선、갑분화CO능인기정자수감소화기형정자솔증고;γ사선여갑분대강저고환중량지수화증고기형정자솔유현저적협동성교호작용.(3)CO능도치백세포총수、혈소판급혈홍단백현저감소,갑분능사림파세포감소.(4)γ사선、분화갑분대유발모사생물효응구유흔현저적협동작용;CO재여기타삼개인소동시작용시대성선능인기현저적영향.
Combined effects on rabbits induced by γ-rays irradiation in combination with inhalationof benzene,toluene and carbon monoxide and the interactive characteristics (antagonistic, additive or synergistic) of these agents were studied.Ninety healthy malerabbits were equally divided into 9 groups,eight of which were test groups assignedaccording to four-factor-two-dose-level orthogonal layout［L8(27)］program and onewas the control.The two dose-levels of the four factors were:for γ-raysirradiation,0.0075Gy/d and 0.0375Gy/d,and for benzene,toluene and carbon monoxide,4015mg/m3 and 18233mg/m3,9030mg/m3 and 40768mg/m3,934mg/m3 and2788mg/m3respectively.The animals were exposed to γ-raysirradiation combined with benzene,toluene and CO vapor 2h a day and 5daysa week for a period of 8 weeks.Experimental results were analysed withthemethod of variance analysis and the test groups were compared.Theresults showed that (1)the interactive factor,the ratio (ω) of the combined effectscaused by the four agentsto the sum of the four independent effects induced by those agents,was 2.16,1.58,2.07,2.67,1.31and 1.18 for dicentric + ring,acentric,aberration cells,total aberration ,micronucleiand SCEs/C in lymphocytes respectively,and the ratio for aberrant spermswas as high as 5.97.The ratios showed that interactions were mainlysynergistic (ω>1).However,for acentric interactions between γ-rays and benzene was antagonistic.(2)The four agents could all obviously cause decrease in testis weight index,and γ-rays,benzene and CO could markedly reduce the number of sperms and increase the ratio ofaberrant sperms.The interactions between γ-rays and benzene for thedecrease in testisweight index and for the elevation of the ratio of aberrant sperms were synergistic.(3) CO could cause decrease in total amount of leucocytes,thrombocytes andhemoglobin,and toluene cause decrease in lymphocytes.(4)Gamma rays irradiationcombined with inhalation of benzene,toluene and CO could produce significantinteractions which were mainly synergistic,and CO,when combined with γ-rays,benzene and toluene,could cause obvious effects on gland.