CAJ | 학술논문

目的为了解细菌感染性心内膜炎在左右心脏的明显不同临床发病率的原因,研究二尖瓣,三尖辩外科手术和金葡菌菌血症后左右心脏组织部分细胞粘附分子和炎症细胞因子的基因的变化.方法26只家兔分别行二尖瓣,三尖瓣手术和经静脉注射金葡菌,术后早期提取左右心室组织RNA,应用RT-PCR技术检测内皮白细胞粘附分子(ELAM-1),巨噬细胞集落刺激因子(MCSF-1)和血小板内皮细胞粘附分子(PECAM-1)基因表达水平的变化.结果左右心室组织的ELAM-1和MCSF-1在二尖瓣和三尖瓣手术后均明显的高表达,左室表达水平高于右室;PECAM-1没有明显的变化.单独注射金葡菌后仅左心室ELAM-1表达水平与对照组相比明显升高.结论感染性心内膜炎的进程中左右心室组织的细胞粘附分子和炎症因子存在着不同的变化特点.
목적위료해세균감염성심내막염재좌우심장적명현불동림상발병솔적원인,연구이첨판,삼첨변외과수술화금포균균혈증후좌우심장조직부분세포점부분자화염증세포인자적기인적변화.방법26지가토분별행이첨판,삼첨판수술화경정맥주사금포균,술후조기제취좌우심실조직RNA,응용RT-PCR기술검측내피백세포점부분자(ELAM-1),거서세포집락자격인자(MCSF-1)화혈소판내피세포점부분자(PECAM-1)기인표체수평적변화.결과좌우심실조직적ELAM-1화MCSF-1재이첨판화삼첨판수술후균명현적고표체,좌실표체수평고우우실;PECAM-1몰유명현적변화.단독주사금포균후부좌심실ELAM-1표체수평여대조조상비명현승고.결론감염성심내막염적진정중좌우심실조직적세포점부분자화염증인자존재착불동적변화특점.
[Objective] There is significant difference in the incidence of left and right infective endocarditis. To obtain a better insight into the molecular pathology of them, this paper studied left and right ventericle cell adhesion molecules and inflammatory mediators's mRNAs expression in rabbits after mitral or tricuspid valve surgery and S.aureus bacteremia. [Methods] 26 rabbits received 5×106 colony forming units Staphylococcus aureus i.v.; or underwent mitral surgery/tricuspid surgery respectively. The hearts were explanted 24 hours after the intervention, total RNAs of left or right ventricles were isolated and mRNA abundance determined by semiquantitative duplex RT-PCR using 18S RNA as internal control. Results were compared to the control group consisting of seven untreated rabbits.[Results] S. aureus bacteremia alone did only lead to significantly changed mRNA levels of ELAM-1 in left ventericles. After mitral and tricuspid valve surgery, ELAM-1 and MCSF-1 mRNA expressions in left and right ventricles were significantly upregulated, their levels in left ventricle were higher than those in right ventricles. [ Conclusions ]There are different changes of cell adhesion moleculers and inflammatory mediators in left and right ventricles in the development of infective endocarditis.