CAJ | 학술논문

利用荧光光谱法、紫外-可见吸收光谱法、圆二色谱法、凝胶电泳法对铬天青S(CAS)与核酸的作用机理进行了探讨,当ct-DNA浓度增加时,铬天青S的荧光光谱呈荧光增强趋势,紫外-可见光谱呈下降趋势,并使ct-DNA圆二色谱正负峰的吸收强度减小.琼脂糖凝胶电泳实验表明,铬天青S-DNA在泳道中清晰可见.将所得结果与溴化乙锭(EB)-DNA体系性质进行对比,得出铬天青S与DNA有发生类似于溴化乙锭的嵌插作用的结论.
이용형광광보법、자외-가견흡수광보법、원이색보법、응효전영법대락천청S(CAS)여핵산적작용궤리진행료탐토,당ct-DNA농도증가시,락천청S적형광광보정형광증강추세,자외-가견광보정하강추세,병사ct-DNA원이색보정부봉적흡수강도감소.경지당응효전영실험표명,락천청S-DNA재영도중청석가견.장소득결과여추화을정(EB)-DNA체계성질진행대비,득출락천청S여DNA유발생유사우추화을정적감삽작용적결론.
The interactions between chrome azurol S (CAS) and DNA were studied by fluorescence spectroscopy,UV-absorption spectroscopy,circular dichroism (CD) spectroscopy and agarose gel electrophoresis method.With an increasing DNA concentration,the fluorescence intensity of CAS increased accordingly.The absorbance of CAS on UV spectra demonstrated a hypochromic effect with an increasing DNA concentration.The CD spectra of DNA indicated a great change both in terms of negative and positive absorption peaks with CAS added.With regard to the agarose gel electrophoresis analysis,the luminescence intensity of CAS-DNA system could be seen clearly.By comparing the above study results with EB-DNA system,we came to conclusion that chrome azurol S and ethidium bromide displayed the same modes when they interacted with DNA.