CAJ | 학술논문

重金属污染是影响水稻生长和稻米品质的重要因素,其中又以镉(Cadmium,Cd)污染最为严重,然而目前对其分子机制以及解决措施却了解甚少.本研究以水稻品种日本晴为材料,采用0.5 mmol/L镉处理水稻幼根48 h,通过运用结合分子镜像选择(mirror orientation selection,MOS)技术的抑制差减杂交(suppression subtractive hybridization,SSH)方法,构建cDNA抑制差减杂交文库,并进行microarray筛选,用于分离应答镉胁迫的差异表达基因.揭示水稻应对镉胁迫处理的分子机理,为水稻耐受镉胁迫基因的分离奠定了基础.
중금속오염시영향수도생장화도미품질적중요인소,기중우이력(Cadmium,Cd)오염최위엄중,연이목전대기분자궤제이급해결조시각료해심소.본연구이수도품충일본청위재료,채용0.5 mmol/L력처리수도유근48 h,통과운용결합분자경상선택(mirror orientation selection,MOS)기술적억제차감잡교(suppression subtractive hybridization,SSH)방법,구건cDNA억제차감잡교문고,병진행microarray사선,용우분리응답력협박적차이표체기인.게시수도응대력협박처리적분자궤리,위수도내수력협박기인적분리전정료기출.
Heavy metal pollution is an important factor in affecting rice growth and quality of rice.Among all kinds of heavy metal pollution,the cadmium pollution is the most serious one.It is not very clear about the mechanisms of rice responding to cadmium and the measures dealing with pollution.In this paper,to investigate the molecular mechanism of rice response to the Cd treatment (0.5 mM) and to search for rice genes associated with these mechanisms,suppression subtractive hybridization and mirror orientation selection were conducted to construct cDNA libraries with Cd and non-Cd treatment rice (Oryza sativa L.,Nipponbare) seedling roots,and cDNA microarray for these two materials was conducted.The results of this study will help to clarify the molecular mechanisms of rice responding cadmium stress,and provide a way of isolating genes involved in heavy-metal resistance in rice.