CAJ | 학술논문

随机选择3个猪场,从9头母猪生下的仔猪中挑选8l头仔猪.随机分成9组,每组又分为3个小组,每小组3头,分别免疫猪瘟组织苗(A)、猪瘟细胞苗(B)和猪瘟脾淋苗(C),各疫苗分别采用1头份、2头份和3头份3个剂量.于25日龄首免疫前后不同时间点采血,分离血清.应用ELISA和IHA两种方法分别对采集的540份血清检测猪瘟抗体.不同类型的猪瘟疫苗免疫试验结果表明:猪瘟脾淋苗免疫效果较好,但不同疫苗之间的抗体水平差异不显著.不同免疫剂量的试验结果显示:3种疫苗以3头份的剂量免疫后抗体反应略高于1头份和2头份,二免后都能获得较高的抗体水平.
수궤선택3개저장,종9두모저생하적자저중도선8l두자저.수궤분성9조,매조우분위3개소조,매소조3두,분별면역저온조직묘(A)、저온세포묘(B)화저온비림묘(C),각역묘분별채용1두빈、2두빈화3두빈3개제량.우25일령수면역전후불동시간점채혈,분리혈청.응용ELISA화IHA량충방법분별대채집적540빈혈청검측저온항체.불동류형적저온역묘면역시험결과표명:저온비림묘면역효과교호,단불동역묘지간적항체수평차이불현저.불동면역제량적시험결과현시:3충역묘이3두빈적제량면역후항체반응략고우1두빈화2두빈,이면후도능획득교고적항체수평.
Eighty-one piglets,which derived from 9 sows of 3 different farms were divided into 9 groups.27 subgroups,with 3 piglets for each subgroup.Each group wag vaccinated with Hog cholera tissue vaccine(A),cell vaccine(B)or spleen vaccine(C)with three dose levels(1×,2×or 3×recommended doses).Blood samples were collected from the 25th day after vaccination for serum.Antibodies were tested by E2 protein baged on ELISA and IHA.The results showed that spleen vaccine seemed to be better than the other two though there was no significant difference among them.Higher dose(3×recommended dose)generally induced hisher antibody response than lower doses.Higher antibody level was observed after the boosting vaccination.