CAJ | 학술논문

目的探讨COPD急性加重期患者外周血中CD4+CD25+调节性T细胞及CRP水平的变化及意义.方法以36例COPD急性加重期患者(COPD组)和36例COPD稳定期患者(对照组一)及36例健康人(对照组二)作为研究对象,采用流式细胞技术检测三组外周血中CD4+CD25+调节性T细胞的表达水平,并常规检测血清CRP水平.结果 COPD组外周血中CD4+CD25+占CD4+T细胞的百分比为(2.56±1.83)%,明显低于对照组一和对照组二(P均<0.01);COPD组CRP水平为(55.7±14.8)mg/L,明显高于对照组一和对照组二(p均<0.01).CD4+CD25+调节性T细胞表达水平与CRP水平呈负相关(r=-0.312,P<0.05).结论 CD4+CD25+调节性T细胞比例减少导致COPD急性加重期患者细胞免疫失调,CRP参与了炎性反应激活过程,可能为COPD急性加重期免疫治疗提供理论依据.
목적 탐토COPD급성가중기환자외주혈중CD4+CD25+조절성T세포급CRP수평적변화급의의.방법 이36례COPD급성가중기환자(COPD조)화36례COPD은정기환자(대조조일)급36례건강인(대조조이)작위연구대상,채용류식세포기술검측삼조외주혈중CD4+CD25+조절성T세포적표체수평,병상규검측혈청CRP수평.결과 COPD조외주혈중CD4+CD25+점CD4+T세포적백분비위(2.56±1.83)%,명현저우대조조일화대조조이(P균<0.01);COPD조CRP수평위(55.7±14.8)mg/L,명현고우대조조일화대조조이(p균<0.01).CD4+CD25+조절성T세포표체수평여CRP수평정부상관(r=-0.312,P<0.05).결론 CD4+CD25+조절성T세포비례감소도치COPD급성가중기환자세포면역실조,CRP삼여료염성반응격활과정,가능위COPD급성가중기면역치료제공이론의거.
Objective To study the changes and significance of CD4+CD25+ regulatory T cells and C-reactive protein(CRP) in patients with acute exacerbation of chronic obstructive pulmonary disease(COPD). Methods Flow cytometry was used to detect the frequency of CD4+CD25+ regulatory T cells in peripheral blood from 36 patients with acute exacerbation of COPD( COPD group) and 36 patients with clinical stability of COPD(control group one)and 36 normal individuals(control group two). The level of CRP was detected routinely. Results The ratio of CD4+CD25+ regulatory T cells number in peripheral blood of COPD group to the total number of CD4+T cell was (2.56±1.83 )%, and it was significantly decreased compared to the other two groups (P all<0.01 ). The level of CRP in COPD group was markedly higher than that in the other two groups (P all<0.01 ). The level of CD4+CD25+ regulatory T cells in patients with acute exacerbation of COPD had negative relation with CRP. Conclusions CD4+CD25+ regulatory T cells participate in inflammatory response. The proportion of CD4+CD25+ regulatory T cells decreases in patients with acute exacerbation of COPD, and it may result in maladjustment of cytoimmunity.