中华消化外科杂志
中華消化外科雜誌
중화소화외과잡지
CHINESE JOURNAL OF DIGESTIVE SURGERY
2010年
6期
447-450
,共4页
陈浩%李建生%英卫东%王伟
陳浩%李建生%英衛東%王偉
진호%리건생%영위동%왕위
肝肿瘤%细胞外基质蛋白-1%转移
肝腫瘤%細胞外基質蛋白-1%轉移
간종류%세포외기질단백-1%전이
Liver neoplasms%Extracellular matrix protein 1%Metastasis
目的 检测细胞外基质蛋白-1(ECM1)在原发性肝癌中的表达,探讨其在肝癌转移中的临床意义.方法 收集2008年6月至2009年12月安徽医科大学附属省立医院手术切除的60例原发性肝癌患者的癌组织及癌旁组织标本和9例肝外伤患者的正常肝组织标本,采用免疫组织化学染色和Western blot法检测ECM1的表达,分析ECM1表达与肝癌临床病理特征之间的关系.率的比较采用x2检验和Fisher确切概率法,均数比较采用t检验.结果 免疫组织化学染色显示ECM1主要表达在细胞质中.肝癌组织中ECM1阳性表达率为73%,显著高于癌旁组织的20%和正常肝组织的22%(x2=34.286,7.044,P＜0.05).ECM1的表达水平与肝癌有无转移和TNM分期有关(x2=5.455,4.275,P＜0.05),而与患者性别、年龄、肿瘤大小、有无包膜、分化程度、血清AFP水平和HBsAg表达等无明显关系(x2=2.841,0.014,0.000,0.734,0.075,0.000,0.031,P＞0.05).Western b1ot检测结果显示肝癌组织中ECM1相对含量为25.49±4.61,显著高于癌旁组织的3.00±0.37和正常肝组织的2.94±0.21(t=31.962,31.699,P＜0.05).结论 ECM1在原发性肝癌组织中的表达高于癌旁和正常肝组织,且与肝癌有无转移和TNM分期有关,提示ECM1可能参与了肝癌的转移,可能是肝癌转移的预测指标之一.
目的 檢測細胞外基質蛋白-1(ECM1)在原髮性肝癌中的錶達,探討其在肝癌轉移中的臨床意義.方法 收集2008年6月至2009年12月安徽醫科大學附屬省立醫院手術切除的60例原髮性肝癌患者的癌組織及癌徬組織標本和9例肝外傷患者的正常肝組織標本,採用免疫組織化學染色和Western blot法檢測ECM1的錶達,分析ECM1錶達與肝癌臨床病理特徵之間的關繫.率的比較採用x2檢驗和Fisher確切概率法,均數比較採用t檢驗.結果 免疫組織化學染色顯示ECM1主要錶達在細胞質中.肝癌組織中ECM1暘性錶達率為73%,顯著高于癌徬組織的20%和正常肝組織的22%(x2=34.286,7.044,P＜0.05).ECM1的錶達水平與肝癌有無轉移和TNM分期有關(x2=5.455,4.275,P＜0.05),而與患者性彆、年齡、腫瘤大小、有無包膜、分化程度、血清AFP水平和HBsAg錶達等無明顯關繫(x2=2.841,0.014,0.000,0.734,0.075,0.000,0.031,P＞0.05).Western b1ot檢測結果顯示肝癌組織中ECM1相對含量為25.49±4.61,顯著高于癌徬組織的3.00±0.37和正常肝組織的2.94±0.21(t=31.962,31.699,P＜0.05).結論 ECM1在原髮性肝癌組織中的錶達高于癌徬和正常肝組織,且與肝癌有無轉移和TNM分期有關,提示ECM1可能參與瞭肝癌的轉移,可能是肝癌轉移的預測指標之一.
목적 검측세포외기질단백-1(ECM1)재원발성간암중적표체,탐토기재간암전이중적림상의의.방법 수집2008년6월지2009년12월안휘의과대학부속성립의원수술절제적60례원발성간암환자적암조직급암방조직표본화9례간외상환자적정상간조직표본,채용면역조직화학염색화Western blot법검측ECM1적표체,분석ECM1표체여간암림상병리특정지간적관계.솔적비교채용x2검험화Fisher학절개솔법,균수비교채용t검험.결과 면역조직화학염색현시ECM1주요표체재세포질중.간암조직중ECM1양성표체솔위73%,현저고우암방조직적20%화정상간조직적22%(x2=34.286,7.044,P＜0.05).ECM1적표체수평여간암유무전이화TNM분기유관(x2=5.455,4.275,P＜0.05),이여환자성별、년령、종류대소、유무포막、분화정도、혈청AFP수평화HBsAg표체등무명현관계(x2=2.841,0.014,0.000,0.734,0.075,0.000,0.031,P＞0.05).Western b1ot검측결과현시간암조직중ECM1상대함량위25.49±4.61,현저고우암방조직적3.00±0.37화정상간조직적2.94±0.21(t=31.962,31.699,P＜0.05).결론 ECM1재원발성간암조직중적표체고우암방화정상간조직,차여간암유무전이화TNM분기유관,제시ECM1가능삼여료간암적전이,가능시간암전이적예측지표지일.
Objective To detect the expression of extracellular matrix protein 1 ( ECM1 ) in primary liver cancer tissues, and explore its clinical significance in liver cancer metastasis. Methods Sixty cases of primary liver cancer tissues and adjacent tissues from 60 patients who were admitted to the Affiliated Provincial Hospital of Anhui Medical University from June 2008 to December 2009 were collected, and nine cases of normal liver tissues were collected from patients with liver trauma as control. The expression of ECM1 and the relationship between ECM1 and clinicopathological features of liver cancer were detected and analyzed using the immunohistochemistry and Western blot. All data were analyzed using the chi-square test, Fisher exact test and t test. Results ECM1 was mainly expressed in the cytoplasm of liver cells. The positive expression rate of ECM1 in liver cancer tissues was 73%, which was significantly higher than those in adjacent tissues (20%) and normal liver tissues (22%)( x2 = 34.286, 7. 044, P ＜ 0.05 ). The expression of ECM1 was correlated with liver cancer metastasis and TNM stages ( x2 = 5. 455, 4.275, P ＜ 0.05), while not with sex, age, size, capsule and differentiation of the tumor,alpha fetoprotein level and the expression of hepatitis B surface antigen ( x2 = 2. 841, 0. 014, 0. 000, 0. 734,0.075, 0.000, 0.031, P＞0.05). The result of Western blot indicated that the relative content of ECM1 in the liver cancer tissues was 25.49 ± 4.61, which was significantly higher than those in adjacent tissues (3.00 ±0.37) and normal liver tissues (2.94 ± 0.21 ) ( t = 31. 962, 31. 699, P ＜ 0. 05 ). Conclusions The expression of ECM1 in liver cancer tissues is higher than those in adjacent and normal liver tissues, and ECM1 expression is correlated with metastasis of liver cancer and TNM stages, which indicate that ECM1 may play a role in the metastasis of liver cancer, and it could be used as an indicator for liver cancer metastasis.
