中华风湿病学杂志
中華風濕病學雜誌
중화풍습병학잡지
CHINESE JOURNAL OF RHEUMATOLOGY
2010年
9期
614-619
,共6页
邓晓莉%钟丽君%邹霞娟%赵金霞%刘湘源%王悦%崔专%孙琳%刘蕊%姚中强%张碧莹
鄧曉莉%鐘麗君%鄒霞娟%趙金霞%劉湘源%王悅%崔專%孫琳%劉蕊%姚中彊%張碧瑩
산효리%종려군%추하연%조금하%류상원%왕열%최전%손림%류예%요중강%장벽형
光谱分析,质量%红斑狼疮,系统性%生物标记物%免疫吸附技术
光譜分析,質量%紅斑狼瘡,繫統性%生物標記物%免疫吸附技術
광보분석,질량%홍반랑창,계통성%생물표기물%면역흡부기술
Spectrum analysis,mass%Lupus erythematosus,systemic%Biomarker%Immunosorbent technique
目的 探索质谱分析和免疫吸附结合来寻找系统性红斑狼疮(SLE)新的血清生物标记物的有效性.方法 采集20例活动期SLE患者(SLE组)免疫吸附(苯丙氨酸吸附柱和DNA吸附柱)前后的血清、10例非活动期SLE患者(本病对照组)、10例类风湿关节炎(RA)患者和10例干燥综合征(SS)患者(他病对照组)以及20名健康志愿者(健康对照组)的血清,与处理和活化后的壳聚糖铜衍生物纳米材料共同孵育.吸附后的纳米材料和质谱仪分析所用基质混合点板,用Axima-CFR plus基质辅助激光解析电离离子源(MALDI)飞行时间质量分析器(TOF)分析得到谱图.经软件分析找出SLE组吸附前存在而吸附后消失的峰(目标质核比峰),将该目标峰在健康对照组中进行验证,找出对照组不存在的目标质核比峰即为SLE的特异峰,再将该特异峰在其他2个对照组中进行检验.采用t检验.结果 研究共发现3个SLE特异峰蛋白,即在吸附前大量存在、在吸附后消失并且在健康对照组中不存在的质核比峰:3136、3264和3326.这些特异峰蛋白在其他2个对照组中均不存在,其相对分子质量(<10 000)均显著小于目前临床检测的SLE血清异常免疫成分如补体、抗双链DNA(dsDNA)抗体、抗Sm抗体等的相对分子质量(>50 000),因此很有可能即为本研究寻找的新的SLE血清标志物.结论 质谱分析结合免疫吸附能够有效发现新的SLE血清学标记物,有利于在健康人群中筛选鉴别出活动期SLE患者.
目的 探索質譜分析和免疫吸附結閤來尋找繫統性紅斑狼瘡(SLE)新的血清生物標記物的有效性.方法 採集20例活動期SLE患者(SLE組)免疫吸附(苯丙氨痠吸附柱和DNA吸附柱)前後的血清、10例非活動期SLE患者(本病對照組)、10例類風濕關節炎(RA)患者和10例榦燥綜閤徵(SS)患者(他病對照組)以及20名健康誌願者(健康對照組)的血清,與處理和活化後的殼聚糖銅衍生物納米材料共同孵育.吸附後的納米材料和質譜儀分析所用基質混閤點闆,用Axima-CFR plus基質輔助激光解析電離離子源(MALDI)飛行時間質量分析器(TOF)分析得到譜圖.經軟件分析找齣SLE組吸附前存在而吸附後消失的峰(目標質覈比峰),將該目標峰在健康對照組中進行驗證,找齣對照組不存在的目標質覈比峰即為SLE的特異峰,再將該特異峰在其他2箇對照組中進行檢驗.採用t檢驗.結果 研究共髮現3箇SLE特異峰蛋白,即在吸附前大量存在、在吸附後消失併且在健康對照組中不存在的質覈比峰:3136、3264和3326.這些特異峰蛋白在其他2箇對照組中均不存在,其相對分子質量(<10 000)均顯著小于目前臨床檢測的SLE血清異常免疫成分如補體、抗雙鏈DNA(dsDNA)抗體、抗Sm抗體等的相對分子質量(>50 000),因此很有可能即為本研究尋找的新的SLE血清標誌物.結論 質譜分析結閤免疫吸附能夠有效髮現新的SLE血清學標記物,有利于在健康人群中篩選鑒彆齣活動期SLE患者.
목적 탐색질보분석화면역흡부결합래심조계통성홍반랑창(SLE)신적혈청생물표기물적유효성.방법 채집20례활동기SLE환자(SLE조)면역흡부(분병안산흡부주화DNA흡부주)전후적혈청、10례비활동기SLE환자(본병대조조)、10례류풍습관절염(RA)환자화10례간조종합정(SS)환자(타병대조조)이급20명건강지원자(건강대조조)적혈청,여처리화활화후적각취당동연생물납미재료공동부육.흡부후적납미재료화질보의분석소용기질혼합점판,용Axima-CFR plus기질보조격광해석전리리자원(MALDI)비행시간질량분석기(TOF)분석득도보도.경연건분석조출SLE조흡부전존재이흡부후소실적봉(목표질핵비봉),장해목표봉재건강대조조중진행험증,조출대조조불존재적목표질핵비봉즉위SLE적특이봉,재장해특이봉재기타2개대조조중진행검험.채용t검험.결과 연구공발현3개SLE특이봉단백,즉재흡부전대량존재、재흡부후소실병차재건강대조조중불존재적질핵비봉:3136、3264화3326.저사특이봉단백재기타2개대조조중균불존재,기상대분자질량(<10 000)균현저소우목전림상검측적SLE혈청이상면역성분여보체、항쌍련DNA(dsDNA)항체、항Sm항체등적상대분자질량(>50 000),인차흔유가능즉위본연구심조적신적SLE혈청표지물.결론 질보분석결합면역흡부능구유효발현신적SLE혈청학표기물,유리우재건강인군중사선감별출활동기SLE환자.
Objective To analyze the efficacy of the combination of MALDI-TOF and immunoadsorption to detect new biomarkers for lupus. Methods Twenty lupus patients at active stage (SLE group), 10 SLE patients in remission (SLE control group), 10 RA patients and 10 PSS patients (other rheumatic disease control group) and 20 healthy volunteers (healthy control group) were enrolled. The serum samples before and after immunoadsorption from SLE group and those from the control groups were co-incubated with activated chitosan copper derivative nano material. The adsorbed nano material was spotted onto the matrix used in MALDI-TOF for analysis by the Axima-CFR plus MALDI-TOF mass spectrometer. T-test was used for statistical analysis. Results MALDI-TOF MS screening showed that three potential protein biomarkers of mass-to-charge (m/z) ratio 3136, 3264, 3326 were found to be very specific for lupus patients: All of them were expressed before immunoadsorption in high quantity and none of them could be detected both after immunoadsorption and in all the three control groups. None of them (<10 000) were in the molecular weight range of the biomarkers used nowadays such as auto antibodies and complement (>50 000). Conclusion The combination of MALDI-TOF and immunoadsorption is effective in the detection of new serum protein biomarkers for lupus and it may be helpful in the screening of SLE patients at active stage from healthy people.
