CAJ | 학술논문

目的检测急性脑损伤昏迷大鼠中脑神经组织中microRNA表达谱的变化特征.方法采用正中液压脑损伤致伤体系,对大鼠进行中等力度致伤,伤后1 h取大鼠中脑组织,经RNA抽提检测后,利用表达谱芯片进行检测,扫描杂交结果并对荧光强度进行标准化后行统计分析. 结果芯片杂交结果表明,损伤昏迷大鼠中脑节段脑组织中,33种microRNA表达上调,38种microRNA表达下降. 结论损伤昏迷大鼠中脑神经组织中microRNA的表达谱出现特征性变化,可能为损伤昏迷的致伤机制,也可能是昏迷保护的神经生物学途径.
목적 검측급성뇌손상혼미대서중뇌신경조직중microRNA표체보적변화특정.방법채용정중액압뇌손상치상체계,대대서진행중등력도치상,상후1 h취대서중뇌조직,경RNA추제검측후,이용표체보심편진행검측,소묘잡교결과병대형광강도진행표준화후행통계분석. 결과 심편잡교결과표명,손상혼미대서중뇌절단뇌조직중,33충microRNA표체상조,38충microRNA표체하강. 결론 손상혼미대서중뇌신경조직중microRNA적표체보출현특정성변화,가능위손상혼미적치상궤제,야가능시혼미보호적신경생물학도경.
Objective To set up a rat model with acute traumatic coma and identify the variation of microRNA in mesencephalon. Methods After rats were injured moderately by central fluid percussion system, tissues of the mesencephalon were removed one hour after injury. RNA of brain tissue of the mesencephalon was isolated for microRNA array by using the exiqon microarray system. The data were analyzed statistically by Genepix Pro 6.0 after hybridization results were scanned and fluorescence intensity standardized. Resets Hybridization results showed 33 microRNAs with up-regulated expressions but 38 microRNAs with down-regulated activity. Conclusion Expression of microRNA array shows marked changes in the tissues of the mesencephalon in rats with traumatic coma, as may be injury mechanism of traumatic coma and also a way of neurobiological protection of coma.